Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterize their protein products and investigate transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).
Learn More: See the Protein Expression chapter of the Protocols & Applications Guide for more information.
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- In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein
- Characterization of mRNA products
- Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction
- Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
- Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.