Polymerase Chain Reaction (PCR) is a powerful research tool for molecular biology laboratories with many different applications. PCR is most commonly associated with the traditional end-point method used to amplify DNA or RNA fragments or detect nucleic acid sequences in a sample. Although traditional PCR can qualitatively detect nucleic acid sequences or genes, real-time quantitative PCR (qPCR) offers a more sensitive, quantitative analysis of a sample.
Use gene expression analysis to confirm your results. qPCR measures the accumulation of PCR product as it occurs during amplification, and can deliver a great deal of information about a sample in a short amount of time.
Promega’s qPCR kits are compatible with all qPCR instrumentation; even competitors. Our qPCR products are also less susceptible to inhibitors. Check out our Data Viewer to see results using different systems, instruments, templates, samples, and more.