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GoTaq® Hot Start Polymerase

Robust PCR with Convenient Room-Temperature Setup

  • Minimize nonspecific amplification and primer-dimers
  • Optimize reaction conditions with supplied Flexi Reaction Buffer and MgCl2 solution
  • Direct-to-gel convenience
  • Supplied at a concentration of 5u/µl

Size

Catalog number selected: M5001

$ 71.00 Your price: Log in

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GoTaq® Hot Start Polymerase
100u
$ 71.00
Your price: Log in
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Performance and Sensitivity of GoTaq® DNA Polymerase with Built-In Hot Start

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl2 to optimize the magnesium concentration in your reactions.

GoTaq® Hot Start Polymerase is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

pcr-guaranteegray-circlePerformance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

7379TC.eps
GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.

Specifications

You are viewing: M5001 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoTaq® Hot Start Polymerase

M500A 1 × 100u

5X Colorless GoTaq® Flexi Buffer

M890A 1 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 1 × 1ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M5005 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 3 × 1.2ml25mM View Product

GoTaq® Hot Start Polymerase

M500B 1 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 4 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 4 × 1ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M5006 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 15 × 1.2ml25mM View Product

GoTaq® Hot Start Polymerase

M500B 5 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 20 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 20 × 1ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M5008 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 60 × 1.2ml25mM View Product

GoTaq® Hot Start Polymerase

M500B 20 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 80 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 80 × 1ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

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