The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-32P]ATP. Just supply the radionucleotide. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.
- Preparing hybridization probes.
- Preparing sequencing primers.
- Labeling marker DNAs and RNAs for autoradiography.
- Preparing probes for gel shift assays.