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Promega Corporation

LigaFast™ Rapid DNA Ligation System

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.

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  • *Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

LigaFast™ Rapid DNA Ligation System

30 reactions
- M8221 $ 88.00 Add to cart

LigaFast™ Rapid DNA Ligation System

150 reactions
- M8225 $ 289.00 Add to cart

Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

2X Rapid Ligation Buffer

(3 × 500μl)
- C6711 $ 85.00 Add to cart

QC Tests

Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Storage Buffer

10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

For product intended use please see Patents & Disclaimers tab.

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