DGKE Kinase Enzyme System

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Easily Screen and Profile DGKE Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: VA7612

$ 545.00
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DGKE Kinase Enzyme System
10µg
$ 545.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The DGKE Kinase Enzyme System contains:

  • DGKE Kinase, 10μg (Human, recombinant, full-length); MW: ~91kDa
  • Dilauroyl-sn-glycerol Substrate, 10mM
  • 5X Reaction Buffer; DTT, 0.1M; Lipid Dilution Buffer II 

Recombinant full-length human DGKE was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Diacylglycerol kinase epsilon (DAG kinase epsilon) is the only member of class III diacylglycerol kinase family, which lacks the E-F hand motifs for Ca2+ binding but possesses a clear selectivity for arachidonoyl-containing species of diacylglycerol. DGK epsilon might decrease signals conducted via arachidonoyl-diacylglycerol or promote the synthesis of phospholipids with certain fatty acids. DGK epsilon contributes to Huntington's disease pathogenesis and its inhibition might be an effective form of therapy for this disease.

Application Note 

NCBI Database Entry

The DGKE Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

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What's in the box?

Item Part # Size

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

DGKE Kinase Enzyme System

VA7612 1 × 10μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

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