BLK Kinase Enzyme System

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Easily Screen and Profile BLK Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: VA7384

$ 545.00
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BLK Kinase Enzyme System
10µg
$ 545.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The BLK Kinase Enzyme System contains:

  • BLK Kinase, 10μg (Human, full-length recombinant). MW: ~84kDa
  • Poly (Glu4, Tyr1) Substrate (1mg/ml)
  • 5X Reaction Buffer, 2.5M MnCl2, 0.1M DTT

Recombinant full-length human BLK was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. BLK, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. BLK is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells.

Application Note

NCBI Database Entry

The BLK Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

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What's in the box?

Item Part # Size

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

BLK Kinase Enzyme System

VA7384 1 × 10μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

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Petra

Petra

Germany