BCR-RET Kinase Enzyme System

4-tubes
View information on global supply logistics
customize-this-small

Easily Screen and Profile BCR-RET Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Size

Additional options

Catalog number selected: VA7030

$ 545.00
Your price:
Add to Cart
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
BCR-RET Kinase Enzyme System
10µg
$ 545.00
Your price: Log in
Change Configuration

Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The BCR-RET Kinase Enzyme System contains:

  • BCR-RET Kinase, 10μg [Human, recombinant; amino acids 1426 (BCR), 713end (RET)]. MW: ~140kDa
  • IGF1Rtide Substrate (1mg/ml), (KKKSPGEYVNIEFG), derived from human IRS-1 protein residues 891902
  • 5X Reaction Buffer, 0.1M DTT

Recombinant human BCR-RET, the fusion protein [BCR (amino acids 1426)-RET (amino acids 713end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BCR-RET is a chimeric fusion of BCR and RET genes generated by a balanced translocation of genes from t(10;22)(q11;q11) and this leads to aberrant activation of RET activity which can transform hematopoietic cells and skew the hematopoietic differentiation program towards the monocytic/macrophage lineage.

Application Note 

NCBI Database Entry 

The BCR-RET Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Specifications

You are viewing: VA7030 Change Configuration

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: VA7031 Change Configuration

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: VA7032 Change Configuration

What's in the box?

Item Part # Size

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

BCR-RET Kinase Enzyme System

VA7030 1 × 10μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

Let's find the product that meets your needs.

Talk to a Scientist

Maria

Maria

Spain