AMPK (A2/B2/G2) Kinase Enzyme System

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Easily Screen and Profile AMPK (A2/B2/G2) Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: VA7027

$ 1,010.00
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AMPK (A2/B2/G2) Kinase Enzyme System
10µg
$ 1,010.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The AMPK (A2/B2/G2) Kinase Enzyme System contains:

  • AMPK (A2/B2/G2) Kinase, 10μg (Human, full-length recombinant). MW: ~92kDa (A2), ~36kDa (B2) and ~65kDa (G2)
  • SAMStide Substrate (1mg/ml), (HMRSAMSGLHVKRR), based on the mouse acetyl-Coenzyme A carboxylase alpha (amino acids 7385)
  • 5X Reaction Buffer, 0.1M DTT, 0.5M AMP Solution

Recombinant full-length human AMPK (combination of A2/B2/G2 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags. AMPK (A2/B2/G2) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits.

Application Note 

A2 Subunit NCBI Database Entry 

B2 Subunit NCBI Datasbase Entry 

G2 Subunit NCBI Database Entry

The AMPK (A2/B2/G2) Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

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What's in the box?

Item Part # Size

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

AMPK (A2/B2/G2) Kinase Enzyme System

VA7027 1 × 10μg

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

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