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Explore the
Biologics Toolkit


Use this guide to find resources and technologies that support antibody therapeutics research and development.

Follow the Analytical Characterization Tools path to find technologies that lead to more precise large molecule characterization.

Navigate Functional Bioassays to find the latest cell-based reporter bioassays to help you stay on the leading edge of the rapid evolution of therapeutic strategies.

Biologics Tool Kit

Analytical Tools


Development of antibody-based therapeutics requires quantitative MOA-based bioassays during all stages of drug research and development. If your interests include peptide mapping, glycan analysis or stability testing of large protein molecules explore our portfolio assays.

Biologics Tool Kit

Tools by Mechanism of Action (MOA)


Incorporating commercially available assays, with industry standard technologies can help your program advance more quickly and efficiently. Explore Promega's growing Bioassay portfolio.

Biologics Tool Kit

Fc Effector Activity


Traditional cell killing assays using primary PBMCs or NK cells are highly relevant measures of antibody Fc effector activity. However, these assays are highly variable and often fail to yield quantitative and reproducible data required for drug discovery and development.

Promega’s Fc Effector Bioassays utilize a reporter-based luciferase readout as a surrogate measure of Fc receptor-mediated signaling. The bioassays are specific, qualified according to ICH guidelines, and correlate with primary cell-based killing assays.

Biologics Tool Kit

T Cell Retargeting


Bispecific antibodies can be used to redirect T cells to kill tumor cells. Assays typically used to measure the activity of T cell bispecific antibodies typically rely on T cell activation and/or target cell killing endpoints. Promega’s T Cell Activation Bioassays shorten the time and reduce the variability associated with traditional readouts such as proliferation and cytokine production. Promega technologies used to measure target cell specific killing enable a larger assay window by eliminating signals coming from non-target cells.

Biologics Tool Kit

Immune Checkpoint Modulation


Immune checkpoint receptors are promising new immunotherapy targets for the treatment of a variety of cancers and autoimmune-mediated disorders.

Promega has developed a suite of reporter-based bioassays to measure the activity of biologics designed to modulate the activity of co-inhibitory and co-stimulatory immune checkpoint receptors.

Biologics Tool Kit

Cytokine Modulation


Therapeutics designed to either enhance or block cytokine activity have traditionally been measured using primary cells with readouts that may include proliferation, FACS, and ELISAs. While physiologically relevant, these assays may suffer from variability and complex workflows.

Employing a luciferase-based reporter assay offers the advantage of a simplified, homogenous workflow with high data quality. Promega has a rapidly growing portfolio of cytokine and growth factor bioassays and cell lines.

Biologics Tool Kit

Antibody Drug Conjugates


Antibody Drug Conjugates (ADC) aim to take advantage of the specificity of monoclonal antibodies (mAbs) to deliver potent cytotoxic drugs selectively to antigen-expressing tumor cells. Promega offers a suite of robust and reproducible assays to monitor the key attributes of ADC including internalization and cell health in real-time or endpoint. Cell Health assays include toxicity, viability, cellular metabolism, and apoptosis.

Biologics Tool Kit

Why Partner with Promega?


Promega is a global leader and partner in providing innovative solutions and technical support for biologics drug development. Promega’s bioassay platform yields quantitative, reproducible, and robust assays with a simple and flexible workflow. We maintain a dynamic pipeline of bioassays and seeks to partner with drug developers in the early stages of development to ensure that our bioassays meet your needs. Explore the benefits of partnering with Promega.

Biologics Tool Kit

Antibody Purification and Conjugation


Antibody Drug Conjugates (ADC) are designed to selectively kill tumor cells via delivery of potent cytotoxic drugs. Promega offers a suite of robust and reproducible assays to monitor the key attributes of ADC including internalization and cell health in real-time or endpoint. Cell Health assays include toxicity, viability, cellular metabolism, and apoptosis.

Biologics Tool Kit

Antibody and Antigen Internalization


Antibody Drug Conjugates (ADC) are designed to selectively kill tumor cells via delivery of potent cytotoxic drugs. Promega offers a suite of robust and reproducible assays to monitor the key attributes of ADC including internalization and cell health in real-time or endpoint. Cell Health assays include toxicity, viability, cellular metabolism, and apoptosis.

Biologics Tool Kit

Endpoint and Kinetic Cell Health Assays


Measure cellular responses to antibody drug conjugates using any cell line. Promega offers a suite of robust and reproducible assays to quantify cellular readouts such as cell viability, cytotoxicity, and apoptosis detection. RealTime kinetic assays and endpoint assays enable multi-dimensional analysis of the impact of ADC products on cellular models.

Biologics Tool Kit

Characterization


Our collection of proteases and other protein analysis reagents for biologics testing include antibody-specific proteases such as IdeS and IdeZ, a variety of general proteases (i.e. g trypsin or Lys-C), and glycosidases. We also have pH-reactive dyes for antibody internalization assays and systems for capture and purification.

Biologics Tool Kit

Proteases/Glycosylases


Our collection of proteases and other protein analysis reagents for biologics testing include antibody-specific proteases such as IdeS and IdeZ, and a variety of general proteases (i.e. g trypsin or Lys-C), glycosidases. We also have pH-reactive dyes for antibody internalization assays and systems for capture and purification.

Biologics Tool Kit

Bioanalytical/PK


Improve protein sample prep workflow with rapid digestion, improved sequence coverage and more data from minimal sample material.

Biologics Tool Kit

Purification


Rapid, simple and robot-friendly antibody purification beads and systems for your manual and automated purification needs.

Biologics Tool Kit

Reference Materials


Characterize proteins and antibodies, study glycosylation, perform digestions faster using alternative proteases and trypsin. Find mass spec reference reagents to quantify and qualify your mass spec instruments.

Biologics Tool Kit

Complement Dependent Cytotoxicity


In Complement Dependent Cytotoxicity (CDC) assays, antibodies bind to surface antigens on the target cell and stimulate a complement cascade, where complement binds to the antibodies and induces a membrane attack complex, lysing the target cell. CDC activity is monitored using assays to measure cell death. Promega’s cytotoxicity assays are robust and reproducible, amenable for measuring the activity of biotherapeutics that act through the CDC pathway.