Nucleic Acid Extraction ›
NGS success depends on the input of high quality DNA or RNA.
Sample Quantitation & Quality Control ›
The amount of DNA or RNA added to your library preparation step dramatically affects your sequencing results.
Library Preparation ›
Minimize sample loss during reaction clean-up steps and protect your RNA during library prep.
Confirmatory Testing ›
Sequence variations and expression level changes need to be verified.
- Step 1
Nucleic Acid Extraction
Nucleic acid extraction is the first step in your NGS workflow. The goal is to purify DNA that meets the highest standards, free of inhibitors and of a certain length is important for success of downstream steps.
Choose from manual or automated purification systems to produce the highest quality, NGS-ready DNA and RNA—even from difficult samples such as FFPE or plant tissues.Tools for Nucleic Acid Extraction for NGS Talk to an NGS Expert
- Step 2
Sample Quantitation & Quality Control
Sample quantitation is necessary to determine how much nucleic acid you have and, therefore, informs how much you should add to library preparation. The quality of your estimation will affect subsequent library preparation efficiency and overall fragment diversity.
Six (and a Half) Reasons to Quantitate Your DNA ›
Find instruments and dyes that provide you with accurate and sensitive fluorescence quantitation of DNA and RNA for many sample types including human FFPE samples.Tools for Quantitation for NGS Talk to an NGS Expert
- Step 3
Library preparation is the process of manipulating your purified DNA into a format that is compatible with a certain sequencing chemistry and instrumentation.
From preventing RNA degradation to efficiently purifying DNA fragments during library preparation, use the highest performing reagents and systems to ensure your NGS success.Tools for Library Preparation for NGS Talk to an NGS Expert
- Step 4
Your NGS experiment doesn't end when the raw data are analyzed—you also need to verify sequence variations or expression changes with confidence. Verify results with confidence using Sanger sequencing, end-point PCR, reverse transcription, qPCR and RT-qPCR systems.
Choose from reagents designed to be sensitive enough to identify your rare variants and robust enough to work with samples that may contain enzyme inhibitors.Tools for Confirmatory Testing for NGS Talk to an NGS Expert