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Your cell lines could be at risk...

Many journals require authentication. Protect your research from contaminated or misidentified cell lines!

Human cultured cell lines are used in many biomedical research and clinical applications, including cancer research, drug discovery, genetics and biobanking. However, misidentification of cell lines continues to plague research, despite prominent scientists calling for authentication.

Scroll down to learn more about 3D optimized Assays
cla-researcher-1
become-a-cell-line-authentication-expert

How can you win? Answer our question of the week, every week. You can also explore the page to learn more about Cell Line Authentication and answer questions along the way. Each correct answer puts you in the running!

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Why do researchers care?

contamination

Researchers are concerned, because:

  1. Misidentified or contaminated cell lines lead to invalidation of data as well as lost time, money and effort.
  2. Authentication of human cell lines is now required or strongly encouraged by many journals and funding agencies.
  3. ANSI Standard ASN-0002-2011 recommends the use of STRs for human cell line authentication.
  4. STR testing is recommended when receiving a cell line from an unreliable source, after ten passages, when preparing a cell bank, and when in doubt of its origin or identity.

When should you authenticate a cell line?

cla-cat-dog
  1. When establishing or acquiring a new cell line.
  2. Within the first week of passaging a new culture.
  3. When starting a new series of experiments.
  4. When routinely passaging cell lines.
  5. When observing inconsistent cell behavior or unexpected results.
  6. When preparing to publish.
  7. When freezing cell stocks to verify purity.
Which journals require authentication?

AACR Journals
Endocrine Society Journals
Society for Endocrinology Journals
Carcinogenesis
Cell Biochemistry and Biophysics
Cell Biology International
International Journal of Cancer
In Vitro Cellular & Developmental

Biology – Animal
Journal of Molecular Biology
Journal of the National Cancer Institute
Molecular Vision
Nature Publishing Group
Neuro-Oncology
Placenta

How does it work?

Cell Line Authentication is achieved through the use of Short Tandem Repeats (STRs). STRs are repetitive sequence elements 3-7 base pairs long scattered throughout the human genome. By amplifying and analyzing these polymorphic loci, then comparing the resulting STR profile to that of a reference sample, the origin of biological samples such as cells or tissues can be identified and verified. The more loci that are amplified, the higher the statistical power of discrimination due to the repeat variation among humans and the human cell lines derived from individuals.

str-analysis-steps-v2

 

Reference: Database of Cross-contaminated or Misidentified Cell Lines - http://iclac.org/databases/cross-contaminations/

What are STR's?

Short tandem repeats or STRs are repetitive sequence elements 3–7 base pairs long scattered throughout the human genome. By amplifying and analyzing these polymorphic loci, then comparing the resulting STR profile to that of a reference sample, the origin of biological samples such as cells or tissues can be identified and verified. The more loci that are amplified, the higher the statistical power of discrimination due to the repeat variation among humans and the human cell lines derived from individuals.

Example of STRs
Tetranucleotide:
----AATGAATGAATG----
Pentanucleotide:
-----AAAGAAAAGAAAAGAAAAGAG-----

Trinucleotide:

---CTTCTTCTTCTTCTT---
research

Now you're a cell line authentication expert!

Download our Info Booklet!

 
But there's always more to learn. Check out more resources on Cell Line Authentication below.

STR products from Promega
Resources
Webinar
Databases

GenePrint® 10 System

The GenePrint® 10 System is a 10-locus multiplex system designed to generate a multi-locus DNA profile by amplifying human genomic DNA extracted from cell pellets, cell culture enrichment media and by direct amplification from storage cards.

  • Amplifies ANSI-0002-Recommended Loci
  • Tolerates higher DNA amounts. Better balance for aneuploid samples
  • Reduced PCR Time. Amplification in less than 1.5 hours
View Product Info

Configuration of the GenePrint® 10 System

geneprint-10

GenePrint® 10 provides co-amplification and four-color detection of ten loci, including all ASN-0002-2011 loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818) plus Amelogenin and D21S11.

Webinar

Maintaining Scientific Integrity Through Cell Line Authentication

Human cell line misidentification is a common problem in the research community that can have significant negative consequences.
As of January 2016, applicants for grants will be required to authenticate key biological resources, including cell lines. This webinar will provide
a brief history of cell line contamination and outline a simple and inexpensive method for authenticating human cell lines.

Watch Now

Databases