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Explore a New Dimension in Research

Take your research to new dimensions with Promega’s assays optimized and validated for 3D cell cultures

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A 3D Experience for your Lab​

Explore cell health assays with validated and optimized protocols for 3D cell culture.

Cell Viability Assays

RealTime-Glo™ MT Cell Viability Assay

Monitor Cell Viability in Real Time

  • Save time, cell culture and reagent costs
  • Rapidly detect changes in cell viability
  • Viability results in spheroids consistent with validated 3D viability assay
Buy Now 3D Protocol

Article

Assaying Cell Health in 3D Cell Culture

Poster

Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction from the Same 3D Spheroids

Citations:

Beta-hairpin hydrogels as scaffolds for high-throughput drug discovery in three-dimensional cell cultureAnal Biochem.2017 Oct 15;535:25-34 ›

The Src family kinase inhibitor dasatinib delays pain-related behaviour and conserves bone in a rat model of cancer-induced bone pain. Sci Rep. 2017; 7: 4792. ›

Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids. J Cell Biol. 2017 Jun 5; 216(6): 1567–1577. ›

CellTiter-Glo® 3D Cell Viability Assay

  • Accurate 3D cytotoxicity determination
  • Easy assay implementation
  • Simple, 30-minute protocol
    Learn More

    Poster

    A Bioluminescent Cell Viability Assay Optimized for 3D Microtissues

    Poster

    Design and Validation of Bioluminescent Assays for 3D Cell Culture Models

    Article

    Luminescent Viability Assays for Magnetically Bioprinted Hepatocyte Spheroids

    Blog Post

    High-Throughput Drug Screening Using 3D Cell Cultures

    Citations:

    Ong, C.S., et al. (2017) Biomaterial-free three-dimensional bioprinting ofcardiac tissue using human induced pluripotent stem cell derived cardiomyocytes. Sci. Reports 7, 4566. ›

    Jung, H.R., et al. (2017) Cell spheroids with enhanced aggressiveness to mimic human liver cancer in vitro and in vivo. Sci. Reports 7, 10499. Pénzes, Á., et al. (2017) Toxicology studies of primycin-sulphate using a three-dimensional (3D) in vitro human liver aggregate model. Toxicol. Lett. 281, 44-52. ›

    Oncogene. A novel three-dimensional high-throughput screening approach identifies inducers of a mutant KRAS selective lethal phenotype.2018 Kota, S., Hou, S., Guerrant, W., Madoux, F., Troutman, S., Fernandez-Vega, V., Alekseeva, N., Madala, N., Scampavia, L., Kissil, J. and Spicer, T.P. ›
    Cytotoxicity Assays

    NEW! LDH-Glo™ Cytotoxicity Assay

    Simple, More Sensitive LDH-based Method to Determine Cytotoxicity

    • Detects LDH release from small numbers of cells, including 3D cell models
    • Monitors cytotoxicity from the same sample over time
    • Provides more data per well through multiplexing with other cell-based assays
    Buy Now 3D Protocol

    Poster

    New Sensitive Luminescent LDH-release Assay that Enables “Real-Time” Sampling from Individual 3D Microtissues

    Article

    Determining Drug Hepatotoxicity in 3D Cultures

    CellTox™ Green Cytotoxicity Assay

    Real-Time Cytotoxicity Detection Assay With Multiplexing Compatibility

    • Accurate cytotoxicity determination in exposures out to 72 hours
    • Useful in visually confirming lysis of 3D structures
    • Multiplex with luminescent assays to obtain more data per well
    Buy Now 3D Protocol

    Poster

    Design and Validation of Bioluminescent Assays for 3D Cell Culture Models

    Poster

    Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction from the Same 3D Spheroids Scientific Poster

    Webinar

    Improving Your Cell-Based Research with Real-Time Assays

    Citations:

    Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids. J Cell Biol. 2017 Jun 5; 216(6): 1567–1577. ›

    Topoisomerase IIα mediates TCF-dependent epithelial–mesenchymal transition in colon cancer. Oncogene. 2016 Sep 22; 35(38): 4990–4999. ›

    Imaging oxygen in neural cell and tissue models by means of anionic cell-permeable phosphorescent nanoparticles. Cell. Mol. Life Sci. (2015) 72: 367. ›
    Apoptosis

    Caspase – Glo® 3/7 Assay

    Apoptosis Detection with Unmatched Speed and Sensitivity

    • Optimized for use with 3D cultures with extended shaking time
    • Simple add-mix-measure protocol—no sample preparation
    • Easily scalable to 96-, 384- and 1,536-well plate formats
    Buy Now 3D Protocol

    Poster

    Design and Validation of Bioluminescent Assays for 3D Cell Culture Models

    Poster

    Factors to Consider for Adapting Off-the-Shelf Assays for use with 3D Culture Models

    App Note

    Caspase-Glo® 3/7 Assay with3D (spheroid) microtissues

    Citations:

    Approach for chemosensitization of cisplatin-resistant ovarian cancer by cucurbitacin B. Tumour Biol. 2016 Jan;37(1):685-98. ›

    RealTime- Glo™ Annexin V Assay

    Simple, Plate-Based Assay for Real-Time Apoptosis Detection

    • No-wash, one-step Annexin V binding assay
    • Non-lytic. Allows continual monitoring of cell state to accurately determine apoptotic onset
    • Scalable for high-throughput 3D screening applications
    Buy Now Supporting Data

    Poster

    Real-Time Assay for Apoptosis using Complementation of Annexin V-Luciferase Subunits

    Webinar

    Improving Your Cell-Based Research with Real-Time Assays

    Supplemental Data

    Citations:

    A novel three-dimensional high-throughput screening approach identifies inducers of a mutant KRAS selective lethal phenotype. Oncogene volume 37, pages 4372–4384 (2018) ›
    Autophagy Assay​

    Assess autophagic activity in both 2D and 3D cell culture models

    • Quantitative, unambiguous LC3 reporter assay
    • Interrogate autophagic flux with 3D models
    • Easy add-mix-measure protocol
    Buy Now Supporting Data

    Poster

    Assessing Autophagic Flux in 2D and 3D Cell Culture Models with a Novel Plate-Based Assay

    Citations:

    Induction of Autophagy and Activation of SIRT-1 Deacetylation Mechanisms Mediate Neuroprotection by the Pomegranate Metabolite Urolithin A in BV2 Microglia and Differentiated 3D Human Neural Progenitor Cells.
    Energy Metabolism Assays​

    NAD/NADH-Glo™ and NADP/NADPH-Glo™ Assays

    Direct and Sensitive Detection of Nicotinamide Adenine Dinucleotides

    • Highly sensitive and rapid bioluminescent assay
    • Evaluate oxidative state of cells in 3D cultures
    • Easily adapted for high-throughput format
    Buy Now Supporting Data

    Poster

    Luminescent Cell Health Assays for Tumor Spheroid Evaluation

    Citations:

    Deshmukh,A. et al (2018) Regulation of cancer stem cell metabolism by secreted frizzled-related protein 4 (sFRP4). Cancers (Basel) 10, E40

    Energy Metabolite Assays

    Sensitive, Simple Bioluminescent Glucose, Lactate, Glutamate and Glutamine Assays

    • Quickly Measure Metabolites in a Variety of Sample Types
    • Detect small changes over time
    • Use to monitor glycolysis in 3D cultures
      Buy Now Supporting Data

      Citations:

      Deshmukh,A. et al (2018) Regulation of cancer stem cell metabolism by secreted frizzled-related protein 4 (sFRP4). Cancers (Basel) 10, E40

      ROS-Glo™ H2O2 Assay

      Monitor H2O2 levels to monitor cellular oxidative stress

      • Fast assay involves a simple two-reagent-addition protocol
      • Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme
      Buy Now Supporting Data

      Citations:

      Bathrinarayanan, P.V., et al. (2018) An investigation into E-cigarette cytotoxicity in-vitro using a novel 3D differentiated co-culture model of human airways. Toxicol. In Vitro 52, 244-64..

      GSH/GSSG-Glo™ Assay

      Measure physiologically relevant GSH/GSSG ratios. Detect and quantify total and reduced glutathione ratios as an indicator of oxidative stress.

      • Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme
      • Measure physiologically relevant GSH/GSSG ratios
      • Simple protocol minimizes sample preparation
        Buy Now Supporting Data

        Citations:

        Fizesan, I. et al (2019) In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires Particle and Fibre Toxicology volume 16

        3D Resources to Support Your Research

        Stay Up-To-Date On the Latest In 3D Technology

        Sign up to join our 3D e-mail list to receive communication about Promega's latest technologies for 3D cell models, resources, webinars and more.

        Guide

        Introduction to 3D Cell Culture

        Webinar

        Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

        Blog Post

        Improving Cancer Drug Screening with 3D Cell Culture

        Get 15% off Promega's assays optimized & validated for 3D cell culture with code 3DPROMEGA. Learn More ›

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