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Explore a New Dimension in Research

Take your research to new dimensions with Promega’s assays optimized and validated for 3D cell cultures

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A 3D Experience for your Lab​

Explore cell health assays with validated and optimized protocols for 3D cell culture.

Cell Viability Assays
Cytotoxicity Assays

NEW! LDH-Glo™ Cytotoxicity Assay

Simple, More Sensitive LDH-based Method to Determine Cytotoxicity

  • Detects LDH release from small numbers of cells, including 3D cell models
  • Monitors cytotoxicity from the same sample over time
  • Provides more data per well through multiplexing with other cell-based assays
Buy Now 3D Protocol
Apoptosis

Caspase – Glo® 3/7 Assay

Apoptosis Detection with Unmatched Speed and Sensitivity

  • Optimized for use with 3D cultures with extended shaking time
  • Simple add-mix-measure protocol—no sample preparation
  • Easily scalable to 96-, 384- and 1,536-well plate formats
Buy Now 3D Protocol

RealTime- Glo™ Annexin V Assay

Simple, Plate-Based Assay for Real-Time Apoptosis Detection

  • No-wash, one-step Annexin V binding assay
  • Non-lytic. Allows continual monitoring of cell state to accurately determine apoptotic onset
  • Scalable for high-throughput 3D screening applications
Buy Now Supporting Data
Autophagy Assay​
Energy Metabolism Assays​

NAD/NADH-Glo™ and NADP/NADPH-Glo™ Assays

Direct and Sensitive Detection of Nicotinamide Adenine Dinucleotides

  • Highly sensitive and rapid bioluminescent assay
  • Evaluate oxidative state of cells in 3D cultures
  • Easily adapted for high-throughput format
Buy Now Supporting Data

Energy Metabolite Assays

Sensitive, Simple Bioluminescent Glucose, Lactate, Glutamate and Glutamine Assays

  • Quickly Measure Metabolites in a Variety of Sample Types
  • Detect small changes over time
  • Use to monitor glycolysis in 3D cultures
    Buy Now Supporting Data

    ROS-Glo™ H2O2 Assay

    Monitor H2O2 levels to monitor cellular oxidative stress

    • Fast assay involves a simple two-reagent-addition protocol
    • Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme
    Buy Now Supporting Data

    GSH/GSSG-Glo™ Assay

    Measure physiologically relevant GSH/GSSG ratios. Detect and quantify total and reduced glutathione ratios as an indicator of oxidative stress.

    • Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme
    • Measure physiologically relevant GSH/GSSG ratios
    • Simple protocol minimizes sample preparation
      Buy Now Supporting Data

      3D Resources to Support Your Research

      Want to talk 3D?

      Looking for further information about Promega's cell health assays and 3D models? Get in touch with a scientist by submitting an inquiry.

      Get 15% off Promega's assays optimized & validated for 3D cell culture with code 3DPROMEGA. Learn More ›