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Water-Glo™ System


Test Water for the Presence of Biological Activity

  • Test process water, freshwater, seawater or wastewater samples
  • Limit of Detection 0.2pg/ml ATP for process or drinking water
  • Broad linear range for applications where bacterial load is low or high

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This product is discontinued
Water-Glo™ System

Rapidly Assess Biomass Viability in Water

Bacteria present in water samples cause issues such as biofilm formation (biofouling) and microbially induced corrosion (MIC). Measuring ATP levels in water samples can be effectively correlated to biomass viability (live microbes present). The Water-Glo™ System uses bioluminescent technology to measure ATP from contaminating live microbes rapidly and easily, offering a highly sensitive measurement that enables you to act quickly to protect processes, human and/or environmental safety, and water quality.



Microbial Analysis of Water Samples for Many Processes and Industries


Detect all viable microbes present:

No bias from composition of growth media, as seen with plate counting.


Get results in minutes:

No need to wait for microbes to grow in media to obtain assay results.


Intervene early:

Sensitive enough to detect contamination in samples with low microbial load.


Monitor effectiveness of biocide treatments:

Broad linear range allows meaningful measurement of microbial contamination in samples with high biomass load.


Use where you need it—field, floor or lab:

Reagent is stable for 12 months at 4°C or 3–4 weeks at 25°C or 1 week at 37°C, once thawed.


Monitor Low Levels for Sensitive Applications


Linear Response of Entire Analytical Range


Frequently Asked Questions

What is ATP and how can you use it to measure bacteria?

ATP is the "energy currency" of all living cells and degrades rapidly when a cell dies. Because of this property, ATP serves as a biomarker for living organisms in a sample and can be used to quantitate the live microbes present.

What is bioluminescence?

Many living organisms, including fireflies, release light energy from chemical reactions. These reactions are catalyzed by proteins in the cell called luciferases, and they require oxygen, ATP and a luciferase substrate. The bioluminescent reaction can be harnessed to detect the required components (such as ATP) in the environment. 

How Does ATP Measurement Compare to Other Forms of Microbial Detection?

Most common methods for detecting microbes in water measure biofouling potential (TDC, AOC or BDOC) or the development of biofouling (pressure drop or BFR). Heterotrophic plate counting directly measures viable microbes, but it is slow and detects less than 10% of viable bacteria. Flow cytometry and PCR improve turnaround time and accuracy but neither method directly measures live bacteria. ATP detection is the only method that directly assesses living cells from any type of microbe, providing an immediate indication of microbial contamination in your sample.

Will the Water-Glo™ Kit Identify What Kind of Contaminants Are in My Sample?

No. The Water-Glo™ Kit detects the presence of living organisms, but does not identify the species. It can be used as an early detection system, and if a bioluminescent signal is detected, other methods, such as PCR or NGS, may be used to identify the specific organism.

Does the Water-Glo™ Kit Allow Me to Perform Assays in Batches?

Yes! The Water-Glo™ chemistry is available in a variety of formats. You can perform single-tube assays or use our larger kit to perform batch assays in 96-well plates. If you need continual, online monitoring of process or industrial water, the Water-Glo™ chemistry is even available as part of the Applitek EZ-ATP® Analyzer. If none of these meet your needs, please contact us to discuss opportunities for custom options.


To request a quote or get more information about the
Water-Glo™ Kit, contact applied@promega.com

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Also Available: Automated, Online ATP Detection

Water-Glo™ Chemistry is also available as part of a fully automated system. The AppliTek EZ-ATP® Instrument with the Water-Glo™ Chemistry provides a total solution for online microbial analysis of water samples. 

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