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Legionella Viability qPCR Kits

Legionella qPCR Kits with Live/Dead Cell Differentiation

  • Get same-day results
  • Rapidly differentiate live and dead cells
  • Identify L. pneumophila or L. spp, L. pneumophila and L. pneum. Serogroup 1
  • Early access material; learn more

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Legionella Viability qPCR Kits

Detect Live Legionella with Speed and Sensitivity

Reliable detection of Legionella pneumophila in water supplies is important to prevent occurrence of Legionnaires’ disease. Legionella testing is commonly performed using culture-based approaches, which often take 7–10 days. PCR-based detection is much faster, but it cannot differentiate between live and dead cells, which can possibly lead to false-positive results.

The Legionella Viability qPCR Kits combine the rapid protocols of PCR with the ability to discriminate between live and dead cells for fast, sensitive and specific Legionella pneumophila detection. We also offer a triplex kit that can detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1 in a single assay.

How the Legionella Viability qPCR Kit Differentiates Live/Dead Cells

The kit contains a cell-impermeable intercalating crosslinker that exclusively binds to DNA from membrane-compromised dead, dying or lysed cells. Once bound, the molecule irreversibly crosslinks DNA, making it unamplifiable in PCR reactions. This ensures that signal from the qPCR detection reaction is exclusively from viable cells and not dead cells, therefore eliminating false-positive results.

How the Legionella pneumophila PCR Kit Differentiates Live/Dead Cells---18076ma
Crosslinker treatment (10µM) greatly reduces false-positive results caused by qPCR signal from dead cells.---18077ma

Crosslinker treatment (10µM) greatly reduces false-positive results caused by qPCR signal from dead cells.


Sample to Results in 6 Hours

Legionella pneumophila Viability qPCR Kits Workflow---18927ma

Created with BioRender.com


Triplex to Detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1

Knowing whether infectious non-pneumophila Legionella species are present can help inform mitigation and response plans. You can conveniently detect Legionella pneumophila and members of the Legionella genus in the same qPCR reaction with the Legionella spp./Legionella pneumophila/L. pneum. Serogroup 1 viability qPCR kit.

Standard curve for detection of L. spp./L. pneumophila/L. pneum. Serogroup 1 using the Legionella Viability qPCR Kits.

Available Kits

  • Legionella spp./Legionella pneumophila/L. pneum. Serogroup 1 Viability qPCR Kit
  • Legionella pneumophila Viability qPCR Kit
  • Viability PCR Crosslinker Kit
  • High-Concentration Viability PCR Crosslinker Kit
  • Prototype Maxwell® Enviro Max Kit

qPCR Kit Contents

  • Legionella qPCR Master Mix
  • 20X Primer/Probe/IPC Master Mix
  • Legionella Quantitative DNA Standard
  • Neutralization Buffer
  • Viability PCR Crosslinker


For Research Use Only. Not for Use in Diagnostic Procedures.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.


Questions?

Contact us at: applied@promega.com

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