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Protocol for Adding HiBiT Tag to an Endogenous Gene Using CRISPR

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Abstract

This protocol describes using clone-free CRISPR/Cas9 gene editing to insert the HiBiT tag into an endogenous locus. From identifying the genomic target, designing guide RNA and donor DNA to delivering the components to the cell and validating the editing event, this illustrated protocol will describe how to use CRISPR to knock-in HiBiT to your protein of interest. Accelerate your research by going from editing to assaying biology in as little as 24 hours using this method.

Promega Corporation

Publication Date: August 2017

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How to Cite This Article

Promega Corporation Protocol for Adding HiBiT Tag to an Endogenous Gene Using CRISPR. [Internet] August 2017. [cited: year, month, date]. Available from: http://www.promega.com/resources/pubhub/applications-notes/protocol-for-adding-hibit-tag-to-an-endogenous-gene-using-crispr/

Promega Corporation Protocol for Adding HiBiT Tag to an Endogenous Gene Using CRISPR. Promega Corporation Web site. http://www.promega.com/resources/pubhub/applications-notes/protocol-for-adding-hibit-tag-to-an-endogenous-gene-using-crispr/ Updated August 2017. Accessed Month Day, Year.