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QuantiFluor® ONE dsDNA System

Fluorescent Quantitation of dsDNA with "Add-and-Read" Convenience

  • Specific quantitation of dsDNA
  • Increased sensitivity over absorbance at 260nm for low-concentration samples
  • Simple to use and automation-friendly

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Catalog number selected: E4871

$ 86.00
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QuantiFluor® ONE dsDNA System
100 reactions
$ 86.00
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Simple, Accurate DNA Quantitation. No Dilutions. No Extra Tubes.

The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) in an automation-friendly, "Add-and-Read" format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It's as easy to use as NanoDrop® absorbance-based methods, but much more sensitive for low-concentration samples. 

QuantiFluor Nanodrop Spectrophotometer DNA Extraction

Improved Sensitivity vs. Absorbance and Other dsDNA Fluorescent Quantitation Systems

The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR.  QuantiFluor® ONE dye sensitivity range is 0.2–400ng/µl (1µl sample in 200µl assay).

DNA Extraction & Purification with Better Sensitivity
Sample* Assay

Quantifluor® ONE dsDNA System

0.2–400ng/µl
0.2–400ng
Qubit® dsDNA HS
0.2–100ng/µl
0.2–100ng
Qubit® dsDNA BR
2–1,000ng/µl
2–1,000ng

*Based on 1µl sample input per assay.


DNA Quantitation Assay Sensitivity and Linearity

The QuantiFluor® ONE System detects as little as 1ng/ml dsDNA per assay using the Quantus™ Fluorometer (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU.

These graphs show representative QuantiFluor® ONE dsDNA System standard curves generated from Lamba or K562 DNA standards in a 96-well plate format. The assay linearity and dynamic range of either the QuantiFluor® ONE Lambda DNA or Genomic K562 DNA standards are shown from a range of 0.2-400ng/µl (1 µl sample in a 200µl assay).

K562 NanoDrop Spectrophotometer DNA Purification
Lambda Fluorescent Dyes DNA Extraction Sensitivity

Easy Setup on Microplate or Single-Tube Fluorometers

System includes all the necessary reagents to quickly set up and quantitate dsDNA, and is easy to set up on microplate or single tube fluorometers, including the following Promega fluorometers.

Quantus Nucleic Acid Quantification Fluorometer

(Use Blue Channel Ex 46 nm, Em 515–575 nm)


How do fluorescent dyes compare to other quantitation methods?

In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.

UV-Absorbance vs Fluorescence

Fluorescence vs qPCR

Specifications

You are viewing: E4871 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

QuantiFluor® ONE dsDNA Dye

E489A 1 × 20ml View Product

QuantiFluor® ONE Lambda DNA

E490B 1 × 80μg

1X TE Buffer (pH 7.5)

E497B 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Specifications

You are viewing: E4870 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

QuantiFluor® ONE dsDNA Dye

E489A 5 × 20ml View Product

QuantiFluor® ONE Lambda DNA

E490A 1 × 400μg

1X TE Buffer (pH 7.5)

E497A 1 × 25ml

SDS

Choose language:

Certificate of Analysis

See all certificates for E4870 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

EE

Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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