N192 pFC34K LgBiT TK-neo Flexi® Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N192 pFC34K LgBiT TK-neo Flexi® Vector Vector sequence reference points: Base pairs 4853 HSV-TK promoter 1-753 SgfI Site 793-800 Barnase coding region 824-1159 EcoICR I 1179-1184 LgBiT 1232-1705 Sv40 late polyadenylation signal 1842-2063 SV40 enhancer and early promoter 2162-2580 SV40 Enhancer 2235-2471 (Reverse) Sv40 Min Ori 2478-2543 EM7 bacterial promoter 2588-2654 Neo-Kan Resistance 2668-3462 Synthetic PolyAdenylation Signal Sequence 3526-3574 ColE1-derived plasmid replication origin 3810-3846 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGATCGC 801 CATGGAATAA GTAAGGAATC CACATGGCAC AGGTTATCAA CACGTTTGAC 851 GGGGTTGCGG ATTATCTTCA GACATATCAT AAGCTACCTG ATAATTACAT 901 TACAAAATCA GAAGCACAAG CCCTCGGCTG GGTGGCATCA AAAGGGAACC 951 TTGCAGACGT CGCTCCGGGG AAAAGCATCG GCGGAGACAT CTTCTCAAAC 1001 AGGGAAGGCA AACTCCCGGG CAAAAGCGGA CGAACATGGC GTGAAGCGGA 1051 TATTAACTAT ACATCAGGCT TCAGAAATTC AGACCGGATT CTTTACTCAA 1101 GCGACTGGCT GATTTACAAA ACAACGGACC ATTATCAGAC CTTTACAAAA 1151 ATCAGATAAT GTTTAATGAC CCCGTGTCGA GCTCAAGGCA GTTCAGGTGG 1201 TGGCGGGAGC GGAGGTGGAG GCTCGAGCGG TGTCTTCACA CTCGAAGATT 1251 TCGTTGGGGA CTGGGAACAG ACAGCCGCCT ACAACCTGGA CCAAGTCCTT 1301 GAACAGGGAG GTGTGTCCAG TTTGCTGCAG AATCTCGCCG TGTCCGTAAC 1351 TCCGATCCAA AGGATTGTCC GGAGCGGTGA AAATGCCCTG AAGATCGACA 1401 TCCATGTCAT CATCCCGTAT GAAGGTCTGA GCGCCGACCA AATGGCCCAG 1451 ATCGAAGAGG TGTTTAAGGT GGTGTACCCT GTGGATGATC ATCACTTTAA 1501 GGTGATCCTG CCCTATGGCA CACTGGTAAT CGACGGGGTT ACGCCGAACA 1551 TGCTGAACTA TTTCGGACGG CCGTATGAAG GCATCGCCGT GTTCGACGGC 1601 AAAAAGATCA CTGTAACAGG GACCCTGTGG AACGGCAACA AAATTATCGA 1651 CGAGCGCCTG ATCACCCCCG ACGGCTCCAT GCTGTTCCGA GTAACCATCA 1701 ACAGCTAAGG CCGCGACTCT AGAGTCGACC TGCAGGCATG CAAGCTGATC 1751 CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT 1801 GAGCAATAAC TAGCATAACC CCTTGGGGCG GCCGCTTCGA GCAGACATGA 1851 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA 1901 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 1951 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT 2001 TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTTAAGCAA GTAAAACCTC 2051 TACAAATGTG GTAAAATCGA ATTTTAACAA AATATTAACG CTTACAATTT 2101 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA 2151 TACGCGGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA 2201 CTTGGTTAGG TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT 2251 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 2301 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 2351 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 2401 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 2451 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 2501 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 2551 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT AATTAACTGT TGACAATTAA 2601 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA 2651 AACCCAGGAG GCAGATCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 2701 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 2751 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 2801 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 2851 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 2901 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 2951 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 3001 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 3051 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 3101 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 3151 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 3201 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA 3251 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 3301 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 3351 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 3401 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 3451 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 3501 CCCAACCTGC CATCACGATG GCCGCAATAA AATATCTTTA TTTTCATTAC 3551 ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGCGATAAG GATCCTCTTT 3601 GCGCTTGCGT TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG 3651 GGGTCAGCAC CGTTTCTGCG GACTGGCTTT CTACCCGGTA TCAGCTCACT 3701 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG 3751 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 3801 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 3851 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 3901 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 3951 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 4001 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC 4051 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 4101 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 4151 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 4201 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 4251 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 4301 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT 4351 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATTTCA 4401 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 4451 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 4501 TAGATCCTTT TATAGTCCGG AAATACAGGA ACGCACGCTG GATGGCCCTT 4551 CGCTGGGATG GTGAAACCAT GAAAAATGGC AGCTTCAGTG GATTAAGTGG 4601 GGGTAATGTG GCCTGTACCC TCTGGTTGCA TAGGTATTCA TACGGTTAAA 4651 ATTTATCAGG CGCGATTGCG GCAGTTTTTC GGGTGGTTTG TTGCCATTTT 4701 TACCTGTCTG CTGCCGTGAT CGCGCTGAAC GCGTTTTAGC GGTGCGTACA 4751 ATTAAGGGAT TATGGTAAAT CCACTTACTG TCTGCCCTCG TAGCCATCGA 4801 GATAAACCGC AGTACTCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA 4851 TCT Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.