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HiBiT-Header

Detect and quantify any tagged protein with a simple, luminescent signal

Tag and Quantify Endogenous Proteins

Monitor Receptor Internalization

Quantify Protein Abundance and Degradation

New HiBiT technology brings the power of bioluminescence to protein analysis.

HiBiT simplifies protein tagging in live cells, providing a streamlined, antibody-free protocol for detecting tagged proteins that requires only a luminometer for detection. With the dynamic range to detect proteins in live cells without overexpression, and the convenience of a single-reagent-addition method, HiBiT technology opens up a universe of possibilities for researchers studying protein biology.

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Choose from 3 HiBiT Detection Systems
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Lytic Method

Sensitive quantification of HiBiT-tagged proteins in cell lysates, IP complexes, or other cell-free systems.

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EXTRACELLULAR METHOD

Used with live cells to quantify surface-expressed or secreted proteins.

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BLOTTING SYSTEM

Detects any tagged protein on a blot in a few minutes with a simple luminescent signal.

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Applications of HiBiT Technology

Tag Endogenous Proteins via CRISPR Insertion—No Cloning Required

HiBiT technology makes CRISPR–mediated tag knock-ins accessible by eliminating the need for molecular cloning prior to insertion and simplifying detection of tagged proteins. The small tag size makes CRISPR insertion efficient and the bioluminescence enables antibody-free detection with the sensitivity to detect endogenous expression.

14381MB

Detect GPCR Internalization without Antibodies

The HiBiT detection method eliminates the need for antibody-based methods for receptor internalization studies. With HiBiT technology, the multiple antibody binding steps and associated washes are eliminated from detection protocols. Simply add the detection reagent and measure a luminescent signal.

14498MB

Quantify Regulated Protein Abundance

Classic epitope tagging methods are limited in throughput or sensitivity, require high-quality antibodies and may only yield semi-quantitative results. HiBiT tagging brings the simplicity and sensitivity of bioluminescence to studies on protein abundance, quantifying proteins down to endogenous levels, even those maintained at low expression levels.

14527MB

WHERE WILL YOUR PROTEIN GLO?