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GoTaq® Probe qPCR and RT-qPCR Systems

PCR Satisfaction Guaranteed

Convenient Master Mixes for Probe-Based Real-Time qPCR and RT-qPCR

  • Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
  • Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
  • Resistant to most PCR inhibitors

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GoTaq® Probe qPCR and RT-qPCR Systems
qPCR/2ml
$ 115.00
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Simple, Sensitive Probe-Based qPCR and RT-qPCR

The GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.

Rapid hot-start activation and processive enzymes make the GoTaq® Probe Systems compatible with most real-time PCR instruments using standard or fast cycling.  Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.

Choose qPCR or RT-qPCR Master Mix Formats

GoTaq® Probe qPCR Master Mix
GoTaq® Probe 1-Step RT-qPCR System
GoTaq® Probe 2-Step RT-qPCR System

Sensitivity and Specificity for qPCR

The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe qPCR Master Mix offers:

  • Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
  • Optimized formulation compatible with running multiplex assays.
  • Room-temperature setup, making it suitable for automation and high-throughput detection.
  • Resistance to a wide range of PCR inhibitors.

 

Cat.#Size10µl Reactions20µl Reactions
A6101
2ml
400 reactions
200 reactions
A6102
10ml
2,000 reactions
1,000 reactions
A6101 Probe-Based qPCR Kit & PCR Master Mix

qPCR amplification from FFPE samples with GoTaq® Probe Master Mix. DNA (10ng) from a lung tumor FFPE sample was amplified under both standard and Fast thermal cycling conditions on a Roche LightCycler 480 instrument using a 384-well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

 

Reverse Transcription and qPCR in One Reaction

The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

Cat.#Size10µl Reactions20µl Reactions
A6120
2ml
400 reactions
200 reactions

A6121

12.5ml

2,500 reactions

1,250 reactions

A6120 One-Step Real-Time RT PCR Reagents Kit 13242MA

Expression levels for the Zmm19 gene in corn. Expression was evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120).

 

Two-Step Reverse Transcription and qPCR

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Cat.#Size10µl Reactions20µl Reactions
A6110
2ml
50 × 20µl RT reactions
and 400 × 10µl
qPCR reactions
50 × 20µl RT reactions
and  200 × 20µl
qPCR reactions
A6110 Two-Step Real-Time Probe-based qPCR System

Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.

 

Performance Tested in qPCR with Many Sample Types and Instruments

We have generated experimental data using different systems, instruments, templates, samples and more. Just select the options below to narrow the selection.

 
131 out of 131 selected
DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on an ABI 7500 instrument. Equivalent Cq values were observed for RNaseP and APP regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.#E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP. The three genes gave equivalent Cq values regardless of cycling conditions used for the experiment.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on an ABI 7500 instrument. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq®1-Step RT-qPCR System (Cat.# A6020) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.#E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on the Bio-Rad CFX96 qPCR instrument. The three genes gave equiavlent Cq values regardless of the thermalcycling conditions used for the experiment.
DNA (10ng) from a lung tissue FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.#E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on the Bio-Rad CFX96 qPCR instrument. The three genes gave equiavlent Cq values regardless of the thermalcycling conditions used for the experiment.
DNA (10ng) from a lung tissue FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a BioRad CFX96 instrument. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a BioRad CFX96 instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a BioRad CFX96 instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a BioRad CFX96 instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. Equivalent Cq values were observed for all genes regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 96 well plate. The three genes have equivalent Cq values reagrdless of the thermalcycing conditions used for the experiment.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. RNaseP and APP had equivalent Cq values regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 96 well plate. The three genes have equivalent Cq values reagrdless of the thermalcycing conditions used for the experiment.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 96 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 96 well plate instrument using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 96 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 instrument with a 96 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for RNaseP and APP regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 384 well plate. RNaseP and APP gave equivalent Cq values regardless of the thermalcycling conditions used for the experiment.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument with a 384 well plate. Equivalent Cq values were observed for RNaseP and APP evaluated regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq(R) Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for APP regardless of cycling conditions used for the experiment.
Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions looking at RNaseP, TERT and APP on a Lightcycler 480 qPCR instrument using a 384 well plate. APP samples showed equivalent Cq values regardless of the thermalcycling conditions used for the experiment.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Roche Lightcycler 480 instrument using a 384 well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Lightcycler 480 instrument with a 384 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for B2M and HPRT1 evaluated regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 384 well plate using both standard and Fast thermalcycling conditions.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on a Roche Lightcycler 480 with a 384 well plate using both standard and Fast thermalcycling conditions. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
DNA (10ng) from whole blood was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument looking at RNaseP, TERT and APP.
Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling condtions looking at RNaseP, TERT and APP on the Stratagene Mx3005P qPCR instrument.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® qPCR Master Mix (Cat.# A6002) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument looking at RNaseP, TERT and APP.
DNA (10ng) from whole blood was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument looking at RNaseP and APP.
Commercially available human K562 genomic DNA (Cat.# E4931) (10ng) was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling condtions looking at RNaseP, TERT and APP on the Stratagene Mx3005P qPCR instrument.
DNA (10ng) from a lung tumor FFPE sample was amplified using the GoTaq® Probe qPCR Master Mix (Cat.# A6102) under both standard and Fast thermalcycling conditions on a Stratagene Mx3005P instrument. Equivalent Cq values were observed for RNaseP and APP regardless of cycling conditions used for the experiment.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of whole blood RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on a Stratagene Mx3005P using both standard and Fast thermalcycling conditions.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of HCT116 cell RNA with the GoTaq® 1-Step RT-qPCR System (Cat.# A6020) on a Stratagene Mx3005P using both standard and Fast thermalcycling conditions.
Gene expression levels for B2M, GNB2L1, and HPRT1 genes were determined via 1-Step RT-qPCR using 10ng of lung tumor FFPE RNA with the GoTaq(R) 1-Step RT-qPCR System (Cat.# A6020) on a Stratagene Mx3005P using both standard and Fast thermalcycling conditions.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a high expressed gene, GNB2L1.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a low expressed gene, HPRT1.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) looking at a moderately expressed gene, B2M.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated by looking at the gene expression levels of three genes (GNB2L1, HPRT1, and B2M) using the GoTaq(R) Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq(R) System was able to detect expression levels all three genes from RNA purified from two different microtissue densities.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020) looking at a high expressed gene, GNB2L1.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020) looking at a low expressed gene, HPRT1.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated via RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020) looking at a high expressed gene, B2M.
HCT116 cells were seeded at two different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified RNA was evaluated by looking at the gene expression levels of three genes (GNB2L1, HPRT1, and B2M) using the GoTaq(R) 1-Step RT-qPCR System (Cat. # A6020). The GoTaq(R) System was able to detect all three genes from RNA purified from two different microtissue densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluted using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101) looking at the APP gene. The GoTaq(R) Probe Master Mix was able to detect APP from three different microtissue cell densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluted using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101) looking at the TERT gene. The GoTaq(R) Probe Master Mix was able to detect TERT from three different microtissue cell densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluted using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101) looking at the RNaseP gene. The GoTaq(R) Probe Master Mix was able to detect RNaseP from three different microtissue cell densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq(R) Probe qPCR Master Mix (Cat. # A6101). The GoTaq(R) Probe Master Mix was able to detect APP, TERT, and RNaseP from gDNA purified from three different microtissue densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluted using the GoTaq(R) qPCR Master Mix (Cat. # A6001) looking at the APP gene. The GoTaq(R) Master Mix was able to detect APP from three different microtissue cell densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluted using the GoTaq(R) qPCR Master Mix (Cat. # A6001) looking at the TERT gene. The GoTaq(R) Master Mix was able to detect TERT from three different microtissue cell densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluted using the GoTaq(R) qPCR Master Mix (Cat. # A6001) looking at the RNaseP gene. The GoTaq(R) Master Mix was able to detect RNaseP from three different microtissue cell densities.
HCT116 cells were seeded at three different densities on GravityPLUS™ hanging drop 96-well plates (Insphero) to form microtissues. Purified gDNA was evaluated using the GoTaq® qPCR Master Mix (Cat. # A6001). The GoTaq® Master Mix was able to detect APP, TERT, and RNaseP from gDNA purified from three different microtissue densities.
Circulating Cell Free DNA (CCF DNA) was purifed from plasma using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) Probe qPCR Master Mix was able to amplify all three genes from purified ccfDNA.
Circulating Cell Free DNA (CCF DNA) was purifed from serum using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) Probe qPCR Master Mix was able to amplify all three genes from purified ccfDNA.
Circulating Cell Free DNA (CCF DNA) was purifed from plasma using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® qPCR Master Mix (Cat. # A6001) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) qPCR Master Mix was able to amplify all three genes from purified ccfDNA.
Circulating Cell Free DNA (CCF DNA) was purifed from serum using the Maxwell® RSC instrument and Maxwell® RSC ccfDNA Plasma kit. Purified DNA was amplified using the GoTaq® qPCR Master Mix (Cat. # A6001) looking at three genes, RNaseP, APP and TERT. The GoTaq(R) qPCR Master Mix was able to amplify all three genes from purified ccfDNA.
Four, tenfold dilutions of corn DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at the cl3036_1 LOC100192652 gene.
Four, tenfold dilutions of soybean DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at the ACPD gene.
Four, tenfold dilutions of Arabidopsis DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at the AT302610 gene.
Expression levels for the EF1a gene in corn were evaluted via 1-step RT-qPCR using the GoTaq® 1-step RT-qPCR System (Cat. # A6020).
Expression levels for the Cons6 gene in soybean were evaluted via 1-step RT-qPCR using the GoTaq® 1-step RT-qPCR System (Cat. # A6020).
Expression levels for the EF1A gene in Arabidopsis were evaluted via 1-step RT-qPCR using the GoTaq® 1-step RT-qPCR System (Cat. # A6020).
Expression levels for the Zmm19 gene in corn were evaluted via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120).
Expression levels for the PEPC17 gene in soybean were evaluted via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120).
Expression levels for the NY-YB1 gene in Arabidopsis were evaluted via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120).
Four, tenfold dilutions of corn DNA were amplified via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at the Zmm19 gene.
Four, tenfold dilutions of soybean DNA were amplified via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at the PEPC17 gene.
Expression levels for the GRAS gene in oil palm seeds were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).
Expression levels for the GAPDH gene in coffee bean were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).
Three, tenfold dilutions of oil palm seed DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) and universal plant primers targeting the ATP-1 gene.
Three, tenfold dilutions of rose seed DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) and universal plant primers targeting the ATP-1 gene.
Three, tenfold dilutions of coffee seed DNA were amplified via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) and universal plant primers targeting the ATP-1 gene.
DNA purified from mouse lung tissue samples was analyzed via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.
DNA purified from mouse kidney tissue samples was analyzed via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.
DNA purified from mouse heart tissue samples was analyzed via qPCR using the GoTaq® qPCR Master Mix (Cat.# A6001) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.
DNA purified from mouse lung tissue samples was analyzed via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® Probe qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.
DNA purified from mouse kidney tissue samples was analyzed via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® Probe qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.
DNA purified from mouse heart tissue samples was analyzed via qPCR using the GoTaq® Probe qPCR Master Mix (Cat.# A6101) looking at three different genes (UBC, β-actin and GUSB). The GoTaq® Probe qPCR Master Mix was able to detect all three genes when 10ng of purified DNA (ReliaPrep™ gDNA Miniprep System) was added to each reaction well.
Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse lung tissue and the GoTaq® 1-Step RT-qPCR System (Cat. # A6020). The GoTaq® 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse lung tissue.
Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse kidney tissue and the GoTaq® 1-Step RT-qPCR System (Cat. # A6020). The GoTaq® 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse kidney tissue.
Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse heart tissue and the GoTaq® 1-Step RT-qPCR System (Cat. # A6020). The GoTaq® 1-Ste RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse heart tissue.
Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse lung tissue and the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq® Probe 1-Step RT-qPCR System was able to detect all three low expressed genes from mouse lung tissue.
Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse kidney tissue and the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq® Probe 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse kidney tissue.
Gene expression levels of B2M, GNB2L1, and HPRT1 were determined via RT-qPCR using 10ng of RNA purified from mouse heart tissue and the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120). The GoTaq® Probe 1-Step RT-qPCR System was able to detect high (B2M), medium (GNB2L1), and low (HPRT1) expressed genes from mouse heart tissue.
Human plasma (300µl) was spiked with inactivated CMV particles, followed by DNA extraction using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the Maxwell® RSC instrument. CMV copies ranging from 10,000 to 100 were detected via qPCR using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Bio-Rad CFX96 instrument.
DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in singleplex (Panels A-C) and triplex (Panel D) reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.
DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in singleplex reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.
DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in singleplex reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.
DNA was purified from Maize powder using the Maxwell® 16 Plant DNA Kit on a Maxwell® 16 instrument. Amplification was performed using maize Mhmg (Cy5), NOS (FAM), and P35S (JOE) assays in triplex reactions using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 Real-Time PCR System.
Gene expression of B2M (VIC), HPRT (FAM), RNaseP (ABY), and GAPDH (JUN) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.
Gene expression of RNaseP (ABY) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.
Gene expression of B2M (VIC), HPRT (FAM), RNaseP (ABY), and GAPDH (JUN) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.
Gene expression of B2M (VIC), HPRT (FAM), RNaseP (ABY), and GAPDH (JUN) was analyzed from commercially available total human colon RNA in quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) on an ABI 7500 FAST Instrument.
Gene expression of RNaseP (ABY), GAPDH (JUN), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from human colon FFPE blocks in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of RNaseP (ABY), GAPDH (JUN), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from HCT116 cells in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of B2M (VIC), HPRT (FAM),GAPDH (JUN), and ß-actin (Cy5) was analyzed from commercially available total human colon RNA in singleplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of B2M (VIC), HPRT (FAM), GAPDH (JUN) and ß-actin (Cy5) was analyzed from 20ng of RNA purified from human lung FFPE blocks in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Gene expression of B2M (VIC), HPRT (FAM), GAPDH (JUN) and ß-actin (Cy5) was analyzed from 20ng of RNA purified from HCT116 cells in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on an ABI 7500 FAST instrument.
Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from commercially available total human genomic DNA and triplex.
Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from commercially available total human genomic DNA and triplex.
Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from commercially available total human genomic DNA using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.
Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from 20ng of DNA purified from human lung FFPE blocks in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.
Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from 20ng of DNA purified from human ccfDNA samples in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.
Expression of ß-actin (Cy5), CYP2D6 (FAM), and RNaseP (VIC) was analyzed from 20ng of DNA purified from human genomic DNA in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on an ABI 7500 FAST instrument.
Gene expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from human lung FFPE blocks in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on a Roche Lightcycler 480 instrument.
Gene expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of RNA purified from HCT116 cells in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on a Roche Lightcycler 480 instrument.
Gene expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of commercially available human colon RNA in singleplex and quadruplex using the GoTaq® Probe 1-Step RT-qPCR System (Cat. # A6120) on a Roche Lightcycler 480 instrument.
Expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of DNA purified from human gDNA in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Roche Lightcycler 480 instrument.
Expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of DNA purified from ccfDNA in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Roche Lightcycler 480 instrument.
Expression of ß-actin (Cy5), HPRT (FAM), and B2M (VIC) was analyzed from 20ng of DNA purified from human lung FFPE blcoks in singleplex and triplex using the GoTaq® Probe qPCR Master Mix (Cat. # A6101) on a Roche Lightcycler 480 instrument.
The 16S rRNA gene found in E. coli was detected from ten-fold serial dilutions of E. coli DNA ranging from 37ng to 3.7×10–3ng using the GoTaq® qPCR Master Mix.
The 16S rRNA gene found in S. aureus was detected from ten fold serial dilutions of S. aureus DNA ranging from 126ng to 0.0126ng using the GoTaq® qPCR Master Mix.
The 16S rRNA gene found in Salmonella enterica was detected from ten fold serial dilutions of Salmonella enterica DNA ranging from 500,000fg to 50fg using the GoTaq® qPCR Master Mix.
The stx1 gene found in E. coli was detected from ten fold serial dilutions of E. coli DNA ranging from 37ng to 3.7pg using the GoTaq® Probe qPCR Master Mix.
The nucA gene found in S. aureus was detected from ten fold serial dilutions of S. aureus DNA ranging from 126ng to 0.0126ng using the GoTaq® Probe qPCR Master Mix.
The ttr gene found in Salmonella enterica was detected from ten fold serial dilutions of Salmonella enterica DNA ranging from 500pg to 0.5pg using the GoTaq® Probe qPCR Master Mix.
The hly gene found in Listeria monocytogenes was detected from ten fold serial dilutions of Listeria monocytogenes DNA ranging from 500pg to 0.5pg using the GoTaq® Probe qPCR Master Mix.
A set of predetermined Armored HIV RNA copies were evaluated via RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120) in order to create a standard curve for HIV copy number determination in samples.
Cell culture medium was spiked with 12,500–50,000 copies of Armored HIV, followed by RNA extraction using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit on the Maxwell® RSC instrument. HIV RNA was detected via qPCR using the GoTaq® Probe 1- Step RT-qPCR System (Cat. # A6120) on a Bio-Rad CFX96 instrument.

Suitable for Different Organisms and Sample Types

DNA/RNA have been isolated with GoTaq® qPCR Systems from the following organisms and sample types. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

Organism Types:Sample Types:
  • Animal
  • Bacteria
  • Fungi
  • Human
  • Parasites
  • Viruses
  • Yeast












  • Blood
  • Cells
  • Feces
  • FFPE
  • Food
  • Human Cerebrospinal Fluid (CSF)
  • Plants
  • Plasma
  • Saliva
  • Serum
  • Sputum
  • Swabs
  • Tissue
  • Urine


Compatible with these Instruments

For further details on instrument compatibility, please reference the appropriate Technical Manual. Instruments not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

  • Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System
  • Applied Biosystems 7300 and 7900HT Real-Time PCR System
  • Applied Biosystems GeneAmp® 5700 Thermal Cycler
  • Applied Biosystems StepOne™ and StepOnePlus™ Real-Time PCR Systems
  • Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
  • Bio-Rad CFX96 Real-Time PCR Detection System
  • Bio-Rad DNA Engine Opticon® and Opticon® 2 Real Time PCR Detection Systems
  • Bio-Rad iCycler® iQ™ and iQ™ 5 Real-Time PCR Detection System
  • Bio-Rad/MJ Research Chromo4™ Real-Time Detector
  • Bio-Rad MyiQ™ Real-Time PCR Detection System
  • Cepheid SmartCycler® System
  • Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer
  • Eppendorf Mastercycler® ep realplex Real-Time PCR System
  • Roche LightCycler® 480 Real-Time PCR System
  • Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems
  • Stratagene Mx4000® Multiplex Quantitative PCR System

Specifications

You are viewing: A6101 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® Probe qPCR Master Mix, 2X

A610A 2 × 1ml

CXR Reference Dye

C541A 1 × 100μl View Product

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6102 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

GoTaq® Probe qPCR Master Mix, 2X

A610A 10 × 1ml

CXR Reference Dye

C541B 2 × 200μl30μM View Product

Nuclease-Free Water

P119E 1 × 13ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6120 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

GoTaq® Probe Master Mix with dUTP

A612A 2 × 1ml

CXR Reference Dye

C541B 1 × 200μl30μM View Product

GoScript™ RT Mix for 1-Step RT-qPCR

M700A 1 × 225μl50X

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6121 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

GoTaq® Probe Master Mix with dUTP

A612B 1 × 12.5ml

CXR Reference Dye

C541C 1 × 500μl30μM View Product

GoScript™ RT Mix for 1-Step RT-qPCR

M700C 1 × 500μl

Nuclease-Free Water

P119E 1 × 13ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Specifications

You are viewing: A6110 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoScript™ 5X Reaction Buffer

A500B 1 × 300μl View Product

GoScript™ Reverse Transcriptase

A501B 1 × 50μl

GoTaq® Probe qPCR Master Mix, 2X

A610A 2 × 1ml

Oligo(dT)15 Primer

C110B 1 × 50μg500μg/ml View Product

PCR Nucleotide Mix

C114G 1 × 200μl10mM View Product

Random Primers

C118B 1 × 50μg View Product

CXR Reference Dye

C541A 1 × 100μl View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

Nuclease-Free Water

P119A 3 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.