Wizard® Genomic DNA Purification Kit Technical Manual
Literature # TM050
The Wizard® Genomic DNA Purification Kit is designed for isolation of DNA from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, Gram-positive and Gram-negative bacteria. The Wizard® Genomic DNA Purification Kit is based on a four-step process. The first step in the purification procedure lyses the cells and the nuclei. For isolation of DNA from white blood cells, this step involves lysis of the red blood cells in the Cell Lysis Solution, followed by lysis of the white blood cells and their nuclei in the Nuclei Lysis Solution. An RNase digestion step may be included at this time; it is optional for some applications. The cellular proteins are then removed by a salt-precipitation step, which precipitates the proteins but leaves the high molecular weight genomic DNA in solution. Finally, the genomic DNA is concentrated and desalted by isopropanol precipitation.
DNA purified with this system is suitable for a variety of applications, such as amplifications, restriction endonuclease digestions and membrane hybridizations (e.g., Southern and dot/slot blots).
Summary of Changes
The following change was made to the 3/19 revision of this document:
Updated instructions for Section 3.D, Tissue Culture Cells, Step 1.d.
Printed in USA. Revised 3/19.