We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Register now for the International Symposium on Human Identification September 23–26.

Casework Direct Kit, Custom

Rapidly Process Sexual Assault Evidence and Low Abundance DNA Samples

  • No-wash protocol maximizes the chance of recovering DNA from trace samples
  • Quickly generates lysates that are compatible with PowerQuant®, Plexor® HY and PowerPlex® Systems
  • Useful for Y-screening and selection of evidentiary samples for STR testing

Size

Catalog number selected: AX4560

Your price:
Add to Cart
This product is discontinued
Add to Helix
Casework Direct Kit, Custom
1 each
Your price: Log in

Rapidly Process Challenging Sample Types

The sheer volume of property crime and sexual assault evidentiary samples submitted to forensic laboratories has compelled many laboratories to actively seek out better solutions for processing these challenging sample types. The Casework Direct Kit is designed to rapidly process swabs from casework samples or cuttings of sexual assault swabs and stained clothing. (Read more in this white paper—download article)

Unlike competing kits, lysates generated with the Casework Direct Kit are compatible with our quantification and STR amplification product lines. These lysates may be amplified with the PowerQuant® or Plexor® HY Systems to screen sexual assault samples for male DNA and to normalize human template for STR amplification with one of the PowerPlex® STR Systems. 

When used with the PowerQuant® System, valuable workflow information such as the presence/absence of male DNA, degradation or potential PCR inhibition is provided.

14303MA-W
ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

How It Works

14304MA-W-a
14304MA-W-b

Add 100–400µl of Casework Direct Solution along with a swab or cutting in the CW Spin Basket assembly, vortex for 5–10 seconds and incubate at 70°C for 30 minutes. Vortex again for 5–10 seconds, and centrifuge at room temperature for 5 minutes at maximum speed in a microcentrifuge to collect the lysate in the 1.5ml microcentrifuge tube. Carefully remove the CW Spin Basket containing the swab or cutting, and discard the spin basket. Quantify 2µl of the lysate using PowerQuant® System or Plexor® HY System. Amplify up to 15µl of the lysate using a PowerPlex® System.

Y-Screening Application

Traditional serology screening of sexual assault samples is laborious and allows limited sensitivity in comparison to male DNA detection by amplification. Using the Casework Direct no-wash protocol, labs can reduce both cost (labor and reagents) as well as the potential DNA loss typically seen in the DNA extraction step. Additionally, conventional screening results offer analysts little predictive power as to what DNA profile may result. Recognizing the limitations and challenges associated with traditional serology methods, new SWGDAM recommendations are now instructing the use of a “Direct to DNA” approach where DNA analysis is performed before serology to maximize the chances of obtaining CODIS eligible profiles. The figure below demonstrates the successful recovery of male DNA from a series of samples that were processed using the Casework Direct Kit.

Detection of Male DNA in Semen Dilution Series
14301MA-W

Detection of male DNA in semen dilution series: Dilutions of human semen were spotted on cotton swabs. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70°C for 30 minutes and centrifuged at max speed for 5 min to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 System. The amplified products were electrophoresed on 3130xl using POP4 with 3kV, 5 sec injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU. The percentage of alleles called is shown on the left axis and the corresponding template amount on the right y-axis. Less than 0.5ng template amount was available for 1:100 and 1:1000 dilution samples. Profiles were called at all dilution except 1:1000 for PowerPlex® Fusion and Y23. 

Dilutions of human semen were spotted on cotton swabs. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged at 16,000 × g for 5 min to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 Systems. The amplified products were analyzed by electrophoresis on the Applied Biosystems® 3130xL Genetic Analyzer using POP-4™ polymer with 3kV, 5 sec injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU. The percentage of alleles called is shown on the primary y-axis and the corresponding template amount on the secondary y-axis. While maximum sample volume was used, less than 0.5ng template amount was available for 1:100 and 1:1000 dilution samples. Full profiles were called at all dilutions except 1:1000 for PowerPlex® Fusion and Y23 Systems.

Casework "Touch" DNA Samples

For the last few years, law enforcement agencies have seen an uptick in property crime evidence submissions, resulting in the need to use significant laboratory resources to test evidence for “touch DNA”—microscopic skin cells containing DNA that naturally rub off when an object, like a car steering wheel, is touched. When DNA is found at a property crime scene, laboratories are often able to match it to a known offender’s sample in CODIS, the national DNA database.

Integration of the Casework Direct Kit, Custom into a laboratory’s workflow provides a rapid and cost effective means to generate, with minimal hands-on time, high quality STR profiles from precious, low abundance samples. When used with the PowerQuant® System, valuable workflow information such as the presence/absence of male DNA degradation or potential PCR inhibition is provided. STR results from several mock casework samples are provided below.

Casework Direct Solution for Touch DNA Samples
14302MA-W-a
14302MA-W-b

Cotton swabs were used to collect touch DNA samples from steering wheel, computer mouse and cell phone surfaces. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged at 16,000 × g for 5 minutes to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 Systems. The amplified products were electrophoresed on the Applied Biosystems® 3500xL Genetic Analyzer using POP4 with 3kV, 5 second injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU.

15166ma

Rapid Casework Sample Processing

Read this white paper to learn more about the developmental validation of the Casework Direct Kit, Custom, for rapid processing of samples ranging from swabs to touch DNA samples.
Download Article

Specifications

What's in the box?

Item Part # SizeAvailable Separately
1-Thioglycerol A208A 1 × 75μl

Casework Direct Reagent, Custom

AX456A 1 × 100ml
5X AmpSolution™ Reagent DM123A 1 × 500μl View Product

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

See all certificates for AX4560 Search
View more results
No results
Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

EE
workflow-background

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-angus

Angus

UK