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Casework Direct Kit, Custom

AX4560

Rapidly Process Sexual Assault Evidence and Low Abundance DNA Samples

  • No-wash protocol maximizes the chance of recovering DNA from trace samples
  • Quickly generates lysates that are compatible with PowerQuant®, Plexor® HY and PowerPlex® Systems
  • Useful for Y-screening and selection of evidentiary samples for STR testing

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Rapidly Process Challenging Sample Types

The sheer volume of property crime and sexual assault evidentiary samples submitted to forensic laboratories has compelled many laboratories to actively seek out better solutions for processing these challenging sample types. The Casework Direct Kit is designed to rapidly process swabs from casework samples or cuttings of sexual assault swabs and stained clothing. (Read more in this white paper—download article)

Unlike competing kits, lysates generated with the Casework Direct Kit are compatible with our quantification and STR amplification product lines. These lysates may be amplified with the PowerQuant® or Plexor® HY Systems to screen sexual assault samples for male DNA and to normalize human template for STR amplification with one of the PowerPlex® STR Systems.

When used with the PowerQuant® System, valuable workflow information such as the presence/absence of male DNA, degradation or potential PCR inhibition is provided.

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ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

How It Works

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100-400µl of Casework Direct Solution is added to the sample placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged for 5 minutes at 16,000 × g to collect the lysate in 1.5ml microfuge tube. The spin basket with solid sample or swab can be discarded. 2µl of this lysate can be quantified using the PowerQuant® System and up to 15µl of the same lysate can be used for PowerPlex® STR amplification.

Y-Screening Application

Traditional serology screening of sexual assault samples is laborious and allows limited sensitivity in comparison to male DNA detection by amplification. By performing amplification, labs can reduce both cost (labor and reagents) as well as the potential DNA loss typically seen in the DNA extraction step. Additionally, conventional screening results offer analysts little predictive power as to what DNA profile may result. Recognizing the limitations and challenges associated with traditional serology methods, new SWGDAM recommendations are now instructing the use of a “Direct to DNA” approach where DNA analysis is performed before serology to maximize the chances of obtaining CODIS eligible profiles. 

Semen Dilution Series
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Detection of male DNA in semen dilution series: Dilutions of human semen were spotted on cotton swabs. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged at max speed for 5 min to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 System. The amplified products were electrophoresed on 3130xl using POP4 with 3kV, 5 sec injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU. The percentage of alleles called is shown on the left axis and the corresponding template amount on the right y-axis. Less than 0.5ng template amount was available for 1:100 and 1:1000 dilution samples. profiles were called at all dilutions except 1:1000 for PowerPlex® Fusion and Y23.

Dilutions of human semen were spotted on cotton swabs. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged at 16,000 × g for 5 min to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 Systems. The amplified products were analyzed by electrophoresis on the Applied Biosystems® 3130xL Genetic Analyzer using POP-4™ polymer with 3kV, 5 sec injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU. The percentage of alleles called is shown on the primary y-axis and the corresponding template amount on the secondary y-axis. While maximum sample volume was used, less than 0.5ng template amount was available for 1:100 and 1:1000 dilution samples. Full profiles were called at all dilutions except 1:1000 for PowerPlex® Fusion and Y23 Systems.

Casework "Touch" DNA Samples

For the last few years, law enforcement agencies have seen in uptake in property crime evidence submissions, resulting in using laboratory resources to property crimes by testing evidence for “touch DNA” - microscopic skin cells containing DNA that naturally rub off when an object, like a car steering wheel, is touched. When DNA is found at a property crime scene, laboratories often match it to a known offender’s sample in CODIS, the national DNA database.

Integration of the Casework Direct Kit, Custom into a laboratory’s workflow provides a rapid and cost effective means to generate with minimal hands on time high quality STR profiles from precious, low abundance samples. When used with the PowerQuant® System, valuable workflow information such as the presence/absence of male DNA degradation or potential PCR inhibition is provided. 

Casework Direct Solution for Touch DNA Samples
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Cotton swabs were used to collect touch DNA samples from steering wheel, computer mouse and cell phone surfaces. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged at 16,000 × g for 5 minutes to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 Systems. The amplified products were electrophoresed on the Applied Biosystems® 3500xL Genetic Analyzer using POP4 with 3kV, 5 second injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU.

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Rapid Casework Sample Processing

Read this white paper to learn more about the developmental validation of the Casework Direct Kit, Custom, for rapid processing of samples ranging from swabs to touch DNA samples.
Download Article

Specifications

What's in the box?

Item Part # SizeAvailable Separately
1-Thioglycerol A208A 1 × 75μl

Casework Direct Reagent, Custom

AX456A 1 × 100ml
5X AmpSolution™ Reagent DM123A 1 × 500μl View Product

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

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