CellTox™ Green Cytotoxicity Assay

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Real-Time Cell Death Assay With Multiplexing Compatibility

  • Accurate cytotoxicity determination in exposures out to 72 hours
  • Flexible protocol allows kinetic analysis or endpoint determination
  • Multiplex with luminescent assays to obtain more data per well

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Catalog number selected: G8741

$ 131.00
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CellTox™ Green Cytotoxicity Assay
10ml
$ 131.00
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CellTox-Green cell death assay Image

Monitoring Cell Death in Real Time Using the CellTox™ Green Cytotoxicity Assay

Cytotoxicity assays based on detection of biomarkers released into the media can underestimate cytotoxicity in long-term exposures of 72 hours or more because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long-term exposure of cells in culture. CellTox Green can be combined with other methods in multiplex assays to determine mechanism of toxicity, and is easily scalable from 96- to 1536-well plate formats.

Learn how the CellTox™ Green Cytotoxicity Assay can be used to assess drug toxicity in organoids.

View Article
Illustration of cell death detection method 10887MB-W

CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.

Perform Kinetic Cytotoxicity Measures to Determine Onset of Toxicity

Dose- and exposure-dependent increases in cytotoxicity

In the experiment shown here, bortezomib caused appreciable cytotoxicity in K562 cells between the 4- and 24-hour time points. Additive fluorescence at later time points indicates continued loss of membrane integrity occurring as a function of cytotoxicity against remaining cells. The CellTox™ Green Dye was added at seeding. 

dose-dependent cytotoxicity data 11114ME-W

Multiplex with Cell Viability Assays for More Informative Data per Well

Measure Cytotoxicity and Viability

Here, CellTox™ Green Dye was mixed with K562 Cells, which were plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72-hour exposure and luminescence (viability) measured. These two inverse measures of cell health resulted in EC50 agreement.

cell viability and cytotoxicity multiplex data 10935MB-W

Determine Appropriate Timing of Caspase Assays

CellTox™ Green can be used in kinetic mode to determine the onset of cytotoxicity followed by multiplexing with the Caspase-Glo® 3/7 Assay in the same sample well to confirm caspase-3/7 activation at the appropriate time.

cytotoxicity and caspase activation data 11115MB-W

Differentiate Between Cytotoxic and Cytostatic Effects

In this example, K562 cells were exposed to Methotrexate, an anti-proliferative compound, for 72 hours. CellTox™ Green Dye was added to determine cytotoxicity, then CellTiter-Glo® Reagent was added to the same sample well for viability determination. A viability assay alone would have misreported the compound as cytotoxic due to less cells in the treated sample compared to the non-treated control, which continued to proliferate. Multiplexing with a cytotoxicity assay reveals no change in signal as a result of compromised membrane integrity, indicating growth inhibition and not cytotoxicity.

cytotoxic and cytostatic effects data 11589TC-W
After several months trying to develop a robust cytotoxicity cell-based assay, we always observed high variability on the upper asymptote of the dose-response curve. Based on Promega’s recommendations, we replaced our previous cytotoxicity detection reagent by the CellTox™ Green Cytotoxicity reagent, which directly solved our issues. The cell-based assay is now fully validated with this reagent in our company.

Dr. Fabian Vandermeers, Sr R&D Technical Leader, Quality Assistance S.A.

Specifications

You are viewing: G8741 Change Configuration

What's in the box?

Item Part # Size

Lysis Solution

G182B 1 × 0.5ml

Assay Buffer

G610A 1 × 10ml

CellTox™ Green Dye, 1,000X

G873A 1 × 20μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G8742 Change Configuration

What's in the box?

Item Part # Size

Lysis Solution

G182B 1 × 0.5ml

Assay Buffer

G610B 1 × 50ml

CellTox™ Green Dye, 1,000X

G873A 5 × 20μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G8743 Change Configuration

What's in the box?

Item Part # Size

Lysis Solution

G182B 1 × 0.5ml

Assay Buffer

G610B 2 × 50ml

CellTox™ Green Dye, 1,000X

G873B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G8731 Change Configuration

What's in the box?

Item Part # Size

CellTox™ Green Dye, 1,000X

G873B 1 × 200μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

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