pH6HTN His6HaloTagŪ T7 Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pH6HTN His6HaloTagŪ T7 Vector sequence reference points: Base pairs 4014 T7 RNA polymerase promoter (-17 to +3) 21-40 His6HaloTagŪ coding region 70-981 His6 region 76-93 HaloTagŪ region 94-981 HaloTagŪ linker region 982-1020 TEV protease recognition region 994-1014 T7 terminator 1132-1179 Beta-lactamase (AmpR) coding region 1513-2373 ColE1-derived plasmid origin of replication 2528-2564 rrnB transcription terminator 3571-3972 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GAATTCATCA TGAAACATCA TCACCATCAC CACGCAGAAA 101 TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT CCTGGGCGAG 151 CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT 201 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC 251 ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC 301 AAATCCGACA AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT 351 CATGGATGCC TTCATCGAAG CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA 401 TTCACGACTG GGGCTCCGCT CTGGGTTTCC ACTGGGCCAA GCGCAATCCA 451 GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC CTATCCCGAC 501 CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA 551 CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG 601 GGTACGCTGC CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA 651 CCATTACCGC GAGCCGTTCC TGAATCCTGT TGACCGCGAG CCACTGTGGC 701 GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG AGCCAGCGAA CATCGTCGCG 751 CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG TCCCGAAGCT 801 GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC GAAGCCGCTC 851 GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT 901 CTGAATCTGC TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC 951 GCGCTGGCTG TCGACGCTCG AGATTTCCGG CGAGCCAACC ACTGAGGATC 1001 TGTACTTTCA GAGCGATAAC GCGATCGCTT CCGAATTCAG AGCTCAACCG 1051 CGGATATCTA GATTTGGGCC CAATTCCTGC AGGATTTTGC GGCCGCTTGC 1101 TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG 1151 CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC 1201 GGTTCGCTTG CTGTCCATAA AACCGCCCAG TCTAGCTATC GCCATGTAAG 1251 CCCACTGCAA GCTACCTGCT TTCTCTTTGC GCTTGCGTTT TCCCTTGTCC 1301 AGATAGCCCA GTAGCTGACA TTCATCCGGG GTCAGCACCG TTTCTGCGGA 1351 CTGGCTTTCT ACGTAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG 1401 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 1451 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 1501 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 1551 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 1601 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 1651 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 1701 TTTTCCAATG ATGAGCACTT TCAAAGTTCT GCTATGTGGC GCGGTATTAT 1751 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 1801 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 1851 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 1901 ACACTGCGGC CAACTTACTT CTGACAACTA TCGGAGGACC GAAGGAGCTA 1951 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 2001 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 2051 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 2101 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 2151 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 2201 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 2251 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 2301 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 2351 CCTCACTGAT TAAGCATTGG TAATTCGAAA TGACCGACCA AGCGACGCCC 2401 AACCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 2451 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 2501 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 2551 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 2601 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 2651 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 2701 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 2751 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 2801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 2851 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 2901 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 2951 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 3001 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 3051 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 3101 CAGAAAAAAA GGATTTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 3151 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 3201 TCAAAAAGGA TCTTCACCTA GATCCTTTTA TAGTCCGGAA ATACAGGAAC 3251 GCACGCTGGA TGGCCCTTCG CTGGGATGGT GAAACCATGA AAAATGGCAG 3301 CTTCAGTGGA TTAAGTGGGG GTAATGTGGC CTGTACCCTC TGGTTGCATA 3351 GGTATTCATA CGGTTAAAAT TTATCAGGCG CGATTGCGGC AGTTTTTCGG 3401 GTGGTTTGTT GCCATTTTTA CCTGTCTGCT GCCGTGATCG CGCTGAACGC 3451 GTTTTAGCGG TGCGTACAAT TAAGGGATTA TGGTAAATCC ACTTACTGTC 3501 TGCCCTCGTA GCCATCGAGA TAAACCGCAG TACTCCGGCC ACGATGCGTC 3551 CGGCGTAGAG GATCGAGATC TTTTCAGCCT GATACAGATT AAATCAGAAC 3601 GCAGAAGCGG TCTGATAAAA CAGAATTTGC CTGGCGGCAG TAGCGCGGTG 3651 GTCCCACCTG ACCCCATGCC GAACTCAGAA GTGAAACGCC GTAGCGCCGA 3701 TGGTAGTGTG GGGTCTCCCC ATGCGAGAGT AGGGAACTGC CAGGCATCAA 3751 ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG 3801 TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG GGAGCGGATT 3851 TGAACGTTGC GAAGCAACGG CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA 3901 TAAACTGCCA GGCATCAAAT TAAGCAGAAG GCCATCCTGA CGGATGGCCT 3951 TTTTGCGTTT CTACAAACTC TTTTGTTTAT TTTTCTAAAT ACATTCAAAT 4001 ATGTATCCGC TCAT Sequence file prepared 02-November-2011.