pRL-CMV Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pRL-CMV Vector* sequence reference points: base pairs 4079 bases CMV Enhancer and Immediate early promoter 7-803 Chimeric intron 860-996 T7 promoter (-17 to +2) 1040-1058 T7 promoter transcription start site 1057 Rluc reporter gene 1068-2003 SV40 late polyadenylation signal 2045-2246 Beta-lactamase (AmpR) coding region 2393-3253 *The cDNA encoding the luciferase from Renilla reniformis is the subject of U.S. Pat. No. 5,292,658 assigned to the University of Georgia Research Foundation, Inc. and sublicensed from Sealite Sciences, Inc., Norcross, GA. The pRL family of Renilla luciferase cDNA vectors is for research use only. Commercial manufacture would require a license from SeaLite Sciences, Inc. The CMV promoter and its use are covered by U.S. Pat. Nos. 5,168,062 and 5,385,839 owned by the University of Iowa Research Foundation, Iowa City, Iowa and licensed FOR RESEARCH USE ONLY. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation. Certain applications of this product may require licenses from others. .. 1 AGATCTTCAA TATTGGCCAT TAGCCATATT ATTCATTGGT TATATAGCAT 51 AAATCAATAT TGGCTATTGG CCATTGCATA CGTTGTATCT ATATCATAAT 101 ATGTACATTT ATATTGGCTC ATGTCCAATA TGACCGCCAT GTTGGCATTG 151 ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 201 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT 251 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT 301 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT 351 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA 401 AGTCCGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA 451 TGCCCAGTAC ATGACCTTAC GGGACTTTCC TACTTGGCAG TACATCTACG 501 TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACACCAAT 551 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT 601 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA 651 AATGTCGTAA TAACCCCGCC CCGTTGACGC AAATGGGCGG TAGGCGTGTA 701 CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCAC 751 TAGAAGCTTT ATTGCGGTAG TTTATCACAG TTAAATTGCT AACGCAGTCA 801 GTGCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA 851 GGCACTGGGC AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA 901 ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT TTCTGATAGG 951 CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC 1001 CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT AATACGACTC 1051 ACTATAGGCT AGCCACCATG ACTTCGAAAG TTTATGATCC AGAACAAAGG 1101 AAACGGATGA TAACTGGTCC GCAGTGGTGG GCCAGATGTA AACAAATGAA 1151 TGTTCTTGAT TCATTTATTA ATTATTATGA TTCAGAAAAA CATGCAGAAA 1201 ATGCTGTTAT TTTTTTACAT GGTAACGCGG CCTCTTCTTA TTTATGGCGA 1251 CATGTTGTGC CACATATTGA GCCAGTAGCG CGGTGTATTA TACCAGACCT 1301 TATTGGTATG GGCAAATCAG GCAAATCTGG TAATGGTTCT TATAGGTTAC 1351 TTGATCATTA CAAATATCTT ACTGCATGGT TTGAACTTCT TAATTTACCA 1401 AAGAAGATCA TTTTTGTCGG CCATGATTGG GGTGCTTGTT TGGCATTTCA 1451 TTATAGCTAT GAGCATCAAG ATAAGATCAA AGCAATAGTT CACGCTGAAA 1501 GTGTAGTAGA TGTGATTGAA TCATGGGATG AATGGCCTGA TATTGAAGAA 1551 GATATTGCGT TGATCAAATC TGAAGAAGGA GAAAAAATGG TTTTGGAGAA 1601 TAACTTCTTC GTGGAAACCA TGTTGCCATC AAAAATCATG AGAAAGTTAG 1651 AACCAGAAGA ATTTGCAGCA TATCTTGAAC CATTCAAAGA GAAAGGTGAA 1701 GTTCGTCGTC CAACATTATC ATGGCCTCGT GAAATCCCGT TAGTAAAAGG 1751 TGGTAAACCT GACGTTGTAC AAATTGTTAG GAATTATAAT GCTTATCTAC 1801 GTGCAAGTGA TGATTTACCA AAAATGTTTA TTGAATCGGA CCCAGGATTC 1851 TTTTCCAATG CTATTGTTGA AGGTGCCAAG AAGTTTCCTA ATACTGAATT 1901 TGTCAAAGTA AAAGGTCTTC ATTTTTCGCA AGAAGATGCA CCTGATGAAA 1951 TGGGAAAATA TATCAAATCG TTCGTTGAGC GAGTTCTCAA AAATGAACAA 2001 TAATTCTAGA GCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG 2051 TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA 2101 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 2151 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG 2201 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT 2251 CGATAAGGAT CCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 2301 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 2351 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 2401 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 2451 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 2501 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 2551 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 2601 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 2651 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 2701 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 2751 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 2801 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 2851 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 2901 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 2951 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 3001 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 3051 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 3101 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 3151 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 3201 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 3251 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 3301 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 3351 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 3401 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3451 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 3501 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 3551 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3601 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 3651 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 3701 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 3751 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 3801 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 3851 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3901 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 3951 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 4001 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4051 TTGCTGGCCT TTTGCTCACA TGGCTCGAC Sequenced updated 20-Dec.-99