This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLF1-N [CMV/Hygro] Vector sequence reference points: Base pairs 5919 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 NanoLuc® protein coding region 1065–1577 Linker 1578–1589 Multiple cloning region 1590–1665 SV40 late poly(A) region 1728–1949 SV40 early enhancer/promoter 2048–2460 Synthetic (Hygr) hygromycin coding region 2491–3528 Synthetic poly(A) signal 3552–3600 β-lactamase (Ampr) coding region 3861–4721 ColE1-derived plasmid replication origin 4876–4912 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGGTC TTCACACTCG AAGATTTCGT TGGGGACTGG 1101 CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC AGGGAGGTGT 1151 GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA 1201 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC 1251 CCGTATGAAG GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT 1301 TAAGGTGGTG TACCCTGTGG ATGATCATCA CTTTAAGGTG ATCCTGCACT 1351 ATGGCACACT GGTAATCGAC GGGGTTACGC CGAACATGAT CGACTATTTC 1401 GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA AGATCACTGT 1451 AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA 1501 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC 1551 TGGCGGCTGT GCGAACGCAT TCTGGCGGGC TCGAGCGGCG CGATCGCTTC 1601 CGAATTCAGA GCTCAACCGC GGATATCTAG ATTTGGGCCC AATTCCTGCA 1651 GGATTTTGCG GCCGCTTGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA 1701 CTAGCATAAC CCCTTGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT 1751 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG 1801 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA 1851 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG 1901 GAGATGTGGG AGGTTTTTTT AAGCAAGTAA AACCTCTACA AATGTGGTAA 1951 AATCGAATTT TAACAAAATA TTAACGCTTA CAATTTCCTG ATGCGGTATT 2001 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG CGGATCTGCG 2051 CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG GTTAGGTACC 2101 TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 2151 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 2201 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 2251 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 2301 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 2351 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 2401 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 2451 CTTTTGCAAA AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGAAGAAGC 2501 CCGAACTCAC CGCTACCAGC GTTGAAAAAT TTCTCATCGA GAAGTTCGAC 2551 AGTGTGAGCG ACCTGATGCA GTTGTCGGAG GGCGAAGAGA GCCGAGCCTT 2601 CAGCTTCGAT GTCGGCGGAC GCGGCTATGT ACTGCGGGTG AATAGCTGCG 2651 CTGATGGCTT CTACAAAGAC CGCTACGTGT ACCGCCACTT CGCCAGCGCT 2701 GCACTACCCA TCCCCGAAGT GTTGGACATC GGCGAGTTCA GCGAGAGCCT 2751 GACATACTGC ATCAGTAGAC GCGCCCAAGG CGTTACTCTC CAAGACCTCC 2801 CCGAAACAGA GCTGCCTGCT GTGTTACAGC CTGTCGCCGA AGCTATGGAT 2851 GCTATTGCCG CCGCCGACCT CAGTCAAACC AGCGGCTTCG GCCCATTCGG 2901 GCCCCAAGGC ATCGGCCAGT ACACAACCTG GCGGGATTTC ATTTGCGCCA 2951 TTGCTGATCC CCATGTCTAC CACTGGCAGA CCGTGATGGA CGACACCGTG 3001 TCCGCCAGCG TAGCTCAAGC CCTGGACGAA CTGATGCTGT GGGCCGAAGA 3051 CTGTCCCGAG GTGCGCCACC TCGTCCATGC CGACTTCGGC AGCAACAACG 3101 TCCTGACCGA CAACGGCCGC ATCACCGCCG TAATCGACTG GTCCGAAGCT 3151 ATGTTCGGGG ACAGTCAGTA CGAGGTGGCC AACATCTTCT TCTGGCGGCC 3201 CTGGCTGGCT TGCATGGAGC AGCAGACTCG CTACTTCGAG CGCCGGCATC 3251 CCGAGCTGGC CGGCAGCCCT CGTCTGCGAG CCTACATGCT GCGCATCGGC 3301 CTGGATCAGC TCTACCAGAG CCTCGTGGAC GGCAACTTCG ACGATGCTGC 3351 CTGGGCTCAA GGCCGCTGCG ATGCCATCGT CCGCAGCGGG GCCGGCACCG 3401 TCGGTCGCAC ACAAATCGCT CGCCGGAGCG CAGCCGTATG GACCGACGGC 3451 TGCGTCGAGG TGCTGGCCGA CAGCGGCAAC CGCCGGCCCA GTACACGACC 3501 GCGCGCTAAG GAGGTAGGTC GAGTTTAGAC TCTAGCCATC ACGATGGCCG 3551 CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG 3601 AATCGATAGC GATAAGGATC CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG 3651 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT 3701 GGCTTTCTAC GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA 3751 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 3801 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 3851 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 3901 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3951 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 4001 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 4051 TTCCAATGAT GAGCACTTTC AAAGTTCTGC TATGTGGCGC GGTATTATCC 4101 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 4151 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 4201 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 4251 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC 4301 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 4351 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 4401 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 4451 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 4501 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 4551 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 4601 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 4651 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 4701 TCACTGATTA AGCATTGGTA ATTCGAAATG ACCGACCAAG CGACGCCCAA 4751 CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4851 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 4901 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 4951 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 5001 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 5051 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 5151 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 5201 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 5251 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 5301 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 5351 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 5451 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 5501 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 5551 AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT ACAGGAACGC 5601 ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA AATGGCAGCT 5651 TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG GTTGCATAGG 5701 TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 5751 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT 5801 TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG 5851 CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC GATGCGTCCG 5901 GCGTAGAGGA TCGAGATCT Sequence file created 5-December-2013.