This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector sequence reference points: Base pairs 7313 CMV immediate early enhancer/promoter 20–761 luc2 reporter gene 865–2514 P2A region 2515–2580 NlucP reporter gene 2581–3219 SV40 late poly(A) region 3259–3480 SV40 early enhancer/promoter 3528–3946 Hygromycin resistance coding region 3971–5008 Synthetic poly(A) sequence 5032–5080 Reporter Vector primer 4 (RVprimer4) binding region 5147–5166 Col EI-derived plasmid replication origin 5404 Synthetic β-lactamase (Ampr) coding region 6195–7055 Synthetic poly(A) signal/transcriptional pause region 7160–7313 Reporter Vector primer 3 (RVprimer3) binding region 7262–7281 1 GGCCTAACTG GCCGGTACCT CAATATTGGC CATTAGCCAT ATTATTCATT 51 GGTTATATAG CATAAATCAA TATTGGCTAT TGGCCATTGC ATACGTTGTA 101 TCTATATCAT AATATGTACA TTTATATTGG CTCATGTCCA ATATGACCGC 151 CATGTTGGCA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG 201 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT 251 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA 301 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT 351 CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 401 GTATCATATG CCAAGTCCGC CCCCTATTGA CGTCAATGAC GGTAAATGGC 451 CCGCCTGGCA TTATGCCCAG TACATGACCT TACGGGACTT TCCTACTTGG 501 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG 551 GCAGTACACC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA 601 GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA 651 CGGGACTTTC CAAAATGTCG TAATAACCCC GCCCCGTTGA CGCAAATGGG 701 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT CGTTTAGTGA 751 ACCGTCAGAT CACTAGAAGC TTTATTGCGG TAGTTTATCA CAGTTAAATT 801 GCTAACGCAG TCAGTGGGCC TCGGCGGCCA AGCTTGGCAA TCCGGTACTG 851 TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG 901 CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC 951 CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC 1001 ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG 1051 CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT 1101 GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC 1151 TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC 1201 GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG 1251 CAAGAAAGGG CTGCAAAAGA TCCTCAACGT GCAAAAGAAG CTACCGATCA 1301 TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA 1351 AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT TCAACGAGTA 1401 CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA 1451 TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC 1501 CGCACCGCTT GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA 1551 CCAGATCATC CCCGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG 1601 GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC 1651 GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA 1701 CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG 1751 CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC 1801 GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA 1851 ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA 1901 CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA 1951 GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT TGGACACCGG 2001 TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA 2051 TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC 2101 GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA 2151 CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG 2201 GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC 2251 AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG ATGCCGGCGA 2301 GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA 2351 AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG 2401 CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA 2451 GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGGCG 2501 GCAAGATCGC CGTGGGAAGC GGAGCTACTA ACTTCAGCCT GCTGAAGCAG 2551 GCTGGAGACG TGGAGGAGAA CCCTGGACCT ATGGTCTTCA CACTCGAAGA 2601 TTTCGTTGGG GACTGGCGAC AGACAGCCGG CTACAACCTG GACCAAGTCC 2651 TTGAACAGGG AGGTGTGTCC AGTTTGTTTC AGAATCTCGG GGTGTCCGTA 2701 ACTCCGATCC AAAGGATTGT CCTGAGCGGT GAAAATGGGC TGAAGATCGA 2751 CATCCATGTC ATCATCCCGT ATGAAGGTCT GAGCGGCGAC CAAATGGGCC 2801 AGATCGAAAA AATTTTTAAG GTGGTGTACC CTGTGGATGA TCATCACTTT 2851 AAGGTGATCC TGCACTATGG CACACTGGTA ATCGACGGGG TTACGCCGAA 2901 CATGATCGAC TATTTCGGAC GGCCGTATGA AGGCATCGCC GTGTTCGACG 2951 GCAAAAAGAT CACTGTAACA GGGACCCTGT GGAACGGCAA CAAAATTATC 3001 GACGAGCGCC TGATCAACCC CGACGGCTCC CTGCTGTTCC GAGTAACCAT 3051 CAACGGAGTG ACCGGCTGGC GGCTGTGCGA ACGCATTCTG GCGAATTCTC 3101 ACGGCTTTCC GCCTGAGGTT GAAGAGCAAG CCGCCGGTAC ATTGCCTATG 3151 TCCTGCGCAC AAGAAAGCGG TATGGACCGG CACCCAGCCG CTTGTGCTTC 3201 AGCTCGCATC AACGTCTAAG GCCGCGACTC TAGAGTCGGG GCGGCCGGCC 3251 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA 3301 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 3351 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA 3401 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT 3451 AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTTT 3501 GCGTATTGGG CGCTCTTCCG CTGATCTGCG CAGCACCATG GCCTGAAATA 3551 ACCTCTGAAA GAGGAACTTG GTTAGCTACC TTCTGAGGCG GAAAGAACCA 3601 GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 3651 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 3701 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 3751 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 3801 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 3851 TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA 3901 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCGATT 3951 CTTCTGACAC TAGCGCCACC ATGAAGAAGC CCGAACTCAC CGCTACCAGC 4001 GTTGAAAAAT TTCTCATCGA GAAGTTCGAC AGTGTGAGCG ACCTGATGCA 4051 GTTGTCGGAG GGCGAAGAGA GCCGAGCCTT CAGCTTCGAT GTCGGCGGAC 4101 GCGGCTATGT ACTGCGGGTG AATAGCTGCG CTGATGGCTT CTACAAAGAC 4151 CGCTACGTGT ACCGCCACTT CGCCAGCGCT GCACTACCCA TCCCCGAAGT 4201 GTTGGACATC GGCGAGTTCA GCGAGAGCCT GACATACTGC ATCAGTAGAC 4251 GCGCCCAAGG CGTTACTCTC CAAGACCTCC CCGAAACAGA GCTGCCTGCT 4301 GTGTTACAGC CTGTCGCCGA AGCTATGGAT GCTATTGCCG CCGCCGACCT 4351 CAGTCAAACC AGCGGCTTCG GCCCATTCGG GCCCCAAGGC ATCGGCCAGT 4401 ACACAACCTG GCGGGATTTC ATTTGCGCCA TTGCTGATCC CCATGTCTAC 4451 CACTGGCAGA CCGTGATGGA CGACACCGTG TCCGCCAGCG TAGCTCAAGC 4501 CCTGGACGAA CTGATGCTGT GGGCCGAAGA CTGTCCCGAG GTGCGCCACC 4551 TCGTCCATGC CGACTTCGGC AGCAACAACG TCCTGACCGA CAACGGCCGC 4601 ATCACCGCCG TAATCGACTG GTCCGAAGCT ATGTTCGGGG ACAGTCAGTA 4651 CGAGGTGGCC AACATCTTCT TCTGGCGGCC CTGGCTGGCT TGCATGGAGC 4701 AGCAGACTCG CTACTTCGAG CGCCGGCATC CCGAGCTGGC CGGCAGCCCT 4751 CGTCTGCGAG CCTACATGCT GCGCATCGGC CTGGATCAGC TCTACCAGAG 4801 CCTCGTGGAC GGCAACTTCG ACGATGCTGC CTGGGCTCAA GGCCGCTGCG 4851 ATGCCATCGT CCGCAGCGGG GCCGGCACCG TCGGTCGCAC ACAAATCGCT 4901 CGCCGGAGCG CAGCCGTATG GACCGACGGC TGCGTCGAGG TGCTGGCCGA 4951 CAGCGGCAAC CGCCGGCCCA GTACACGACC GCGCGCTAAG GAGGTAGGTC 5001 GAGTTTAAAC TCTAGAACCG GTCATGGCCG CAATAAAATA TCTTTATTTT 5051 CATTACATCT GTGTGTTGGT TTTTTGTGTG TTCGAACTAG ATGCTGTCGA 5101 CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG 5151 GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC 5201 TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC 5251 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 5301 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 5351 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 5401 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 5451 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 5501 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 5551 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 5601 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 5651 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 5701 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 5751 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 5801 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 5851 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 5901 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 5951 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 6001 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 6051 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 6101 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 6151 AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA 6201 GTGCTTGATC AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT 6251 CCATAGTGGC CTGACTCCCC GTCGTGTAGA TCACTACGAT TCGTGAGGGC 6301 TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC CGCGTTCACC 6351 GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA 6401 GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT 6451 CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT 6501 GGCCATTGCT ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT 6551 CGTTCAACTC TGGTTCCCAG CGGTCAAGCC GGGTCACATG ATCACCCATA 6601 TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG TTGTCAGAAG 6651 TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT 6701 CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC 6751 TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG 6801 CCCGGCGTCT ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG 6851 TGCTCATCAT CGGGAATCGT TCTTCGGGGC GGAAAGACTC AAGGATCTTG 6901 CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC CCAGTTGATC 6951 TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA 7001 AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG 7051 CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA 7101 GTACGTCTCT CAAGGATAAG TAAGTAATAT TAAGGTACGG GAGGTATTGG 7151 ACAGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT 7201 TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA AACAAAACGA 7251 AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC 7301 AGAACATTTC TCT Sequence file created 18-November-2014.