pNL2.1[Nluc/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNL2.1[Nluc/Hygro] Vector sequence reference points: Base pairs 4709 Multiple cloning region 1-70 Nanoluc (Nluc) reporter gene 100-615 SV40 late poly(A) region 655-876 SV40 early enhancer/promoter 924-1342 Synthetic hygromycin (HygR) coding region 1367-2404 Synthetic poly(A) region 2428-2476 Reporter vector primer 4 (RVPrimer4) binding region 2543-2562 ColE1-derived plasmid replication origin 2800 Synthetic beta-lactamase (Ampr)coding region 3591-4451 Synthetic polyadenylation signal/transcriptional pause region 4556-4709 Reporter vector primer 3 (RVPrimer3) binding region 4658-4677 .. 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA 101 TGGTCTTCAC ACTCGAAGAT TTCGTTGGGG ACTGGCGACA GACAGCCGGC 151 TACAACCTGG ACCAAGTCCT TGAACAGGGA GGTGTGTCCA GTTTGTTTCA 201 GAATCTCGGG GTGTCCGTAA CTCCGATCCA AAGGATTGTC CTGAGCGGTG 251 AAAATGGGCT GAAGATCGAC ATCCATGTCA TCATCCCGTA TGAAGGTCTG 301 AGCGGCGACC AAATGGGCCA GATCGAAAAA ATTTTTAAGG TGGTGTACCC 351 TGTGGATGAT CATCACTTTA AGGTGATCCT GCACTATGGC ACACTGGTAA 401 TCGACGGGGT TACGCCGAAC ATGATCGACT ATTTCGGACG GCCGTATGAA 451 GGCATCGCCG TGTTCGACGG CAAAAAGATC ACTGTAACAG GGACCCTGTG 501 GAACGGCAAC AAAATTATCG ACGAGCGCCT GATCAACCCC GACGGCTCCC 551 TGCTGTTCCG AGTAACCATC AACGGAGTGA CCGGCTGGCG GCTGTGCGAA 601 CGCATTCTGG CGTAAGGCCG CGACTCTAGA GTCGGGGCGG CCGGCCGCTT 651 CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG 701 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 751 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC 801 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG 851 CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTTTGCGT 901 ATTGGGCGCT CTTCCGCTGA TCTGCGCAGC ACCATGGCCT GAAATAACCT 951 CTGAAAGAGG AACTTGGTTA GCTACCTTCT GAGGCGGAAA GAACCAGCTG 1001 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 1051 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA 1101 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 1151 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 1201 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 1251 TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG 1301 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCGATTCTTC 1351 TGACACTAGC GCCACCATGA AGAAGCCCGA ACTCACCGCT ACCAGCGTTG 1401 AAAAATTTCT CATCGAGAAG TTCGACAGTG TGAGCGACCT GATGCAGTTG 1451 TCGGAGGGCG AAGAGAGCCG AGCCTTCAGC TTCGATGTCG GCGGACGCGG 1501 CTATGTACTG CGGGTGAATA GCTGCGCTGA TGGCTTCTAC AAAGACCGCT 1551 ACGTGTACCG CCACTTCGCC AGCGCTGCAC TACCCATCCC CGAAGTGTTG 1601 GACATCGGCG AGTTCAGCGA GAGCCTGACA TACTGCATCA GTAGACGCGC 1651 CCAAGGCGTT ACTCTCCAAG ACCTCCCCGA AACAGAGCTG CCTGCTGTGT 1701 TACAGCCTGT CGCCGAAGCT ATGGATGCTA TTGCCGCCGC CGACCTCAGT 1751 CAAACCAGCG GCTTCGGCCC ATTCGGGCCC CAAGGCATCG GCCAGTACAC 1801 AACCTGGCGG GATTTCATTT GCGCCATTGC TGATCCCCAT GTCTACCACT 1851 GGCAGACCGT GATGGACGAC ACCGTGTCCG CCAGCGTAGC TCAAGCCCTG 1901 GACGAACTGA TGCTGTGGGC CGAAGACTGT CCCGAGGTGC GCCACCTCGT 1951 CCATGCCGAC TTCGGCAGCA ACAACGTCCT GACCGACAAC GGCCGCATCA 2001 CCGCCGTAAT CGACTGGTCC GAAGCTATGT TCGGGGACAG TCAGTACGAG 2051 GTGGCCAACA TCTTCTTCTG GCGGCCCTGG CTGGCTTGCA TGGAGCAGCA 2101 GACTCGCTAC TTCGAGCGCC GGCATCCCGA GCTGGCCGGC AGCCCTCGTC 2151 TGCGAGCCTA CATGCTGCGC ATCGGCCTGG ATCAGCTCTA CCAGAGCCTC 2201 GTGGACGGCA ACTTCGACGA TGCTGCCTGG GCTCAAGGCC GCTGCGATGC 2251 CATCGTCCGC AGCGGGGCCG GCACCGTCGG TCGCACACAA ATCGCTCGCC 2301 GGAGCGCAGC CGTATGGACC GACGGCTGCG TCGAGGTGCT GGCCGACAGC 2351 GGCAACCGCC GGCCCAGTAC ACGACCGCGC GCTAAGGAGG TAGGTCGAGT 2401 TTAAACTCTA GAACCGGTCA TGGCCGCAAT AAAATATCTT TATTTTCATT 2451 ACATCTGTGT GTTGGTTTTT TGTGTGTTCG AACTAGATGC TGTCGACCGA 2501 TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC 2551 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT 2601 AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 2651 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 2701 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 2751 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 2801 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 2851 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 2901 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 2951 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 3001 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 3051 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT 3101 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 3151 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 3201 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 3251 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 3301 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 3351 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT 3401 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 3451 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 3501 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 3551 TGGTCTGACA GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC 3601 TTGATCAGTG AGGCACCGAT CTCAGCGATC TGCCTATTTC GTTCGTCCAT 3651 AGTGGCCTGA CTCCCCGTCG TGTAGATCAC TACGATTCGT GAGGGCTTAC 3701 CATCAGGCCC CAGCGCAGCA ATGATGCCGC GAGAGCCGCG TTCACCGGCC 3751 CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG AGCGAAGAAG 3801 TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG 3851 ATGCTAGAGT AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC 3901 ATTGCTACTG GCATCGTGGT ATCACGCTCG TCGTTCGGTA TGGCTTCGTT 3951 CAACTCTGGT TCCCAGCGGT CAAGCCGGGT CACATGATCA CCCATATTAT 4001 GAAGAAATGC AGTCAGCTCC TTAGGGCCTC CGATCGTTGT CAGAAGTAAG 4051 TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC ACAATTCTCT 4101 TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA 4151 CCAAGTCGTT TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG 4201 GCGTCTATAC GGGACAACAC CGCGCCACAT AGCAGTACTT TGAAAGTGCT 4251 CATCATCGGG AATCGTTCTT CGGGGCGGAA AGACTCAAGG ATCTTGCCGC 4301 TATTGAGATC CAGTTCGATA TAGCCCACTC TTGCACCCAG TTGATCTTCA 4351 GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA CAGGCAAGCA 4401 AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA 4451 TACTCGTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC 4501 GTCTCTCAAG GATAAGTAAG TAATATTAAG GTACGGGAGG TATTGGACAG 4551 GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG 4601 TGTGAATCGA TAGTACTAAC ATACGCTCTC CATCAAAACA AAACGAAACA 4651 AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA 4701 CATTTCTCT Sequence file prepared 24-January-2012.