pGloSensor(TM)-10F[TEV] Control Plasmid This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGloSensor(TM)-10F[TEV] Control Plasmid sequence reference points: Base pairs 4989 SP6 RNA polymerase promoter (–17 to +1) 23–42 T7 RNA polymerase promoter (–17 to +1)* 46–63 Barley yellow dwarf virus (BYDV) 5´ untranslated region 65–201 SgfI recognition site 202–209 GloSensor(TM)[TEV] protein-coding region 211–1875 NheI recognition site 1147–1152 PmeI recognition site 1870–1877 BYDV 3´ translation enhancer 1896–2002 T7 terminator 2107–2154 Beta-lactamase (Ampr) coding region 2488–3348 Origin of replication 3503–3539 cer site (site for E. coli XerCD recombinase) 4210–4495 rrnB transcription terminator 4546–4947 *The consensus T7 RNA polymerase promoter sequence extends only to +1 and does not match many standard T7 promoter primers available for sequencing applications. Promega .. 1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA 51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG 101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA 151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG 201 CGCGATCGCC ATGGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG 251 GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC 301 GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA 351 CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG 401 CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC 451 GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA 501 ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA 551 CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA 601 GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT TGGACACTGG 651 TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA 701 TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC 751 GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA 801 CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG 851 GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC 901 AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG ATGCCGGCGA 951 GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA 1001 AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG 1051 CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA 1101 GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGCTA 1151 GCGAGAACCT GTACTTCCAG AGCGGATCTG CCAAAAACAT TAAGAAGGGC 1201 CCAGCGCCAT TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA 1251 CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC GCCTTTACCG 1301 ACGCACATAT CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC 1351 GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA CAAACCATCG 1401 GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG 1451 GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC 1501 GAGCGCGAGC TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT 1551 CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA AAGAAGCTAC 1601 CGATCATACA AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC 1651 TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC CCGGCTTCAA 1701 CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC 1751 TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA 1801 CCGCACCGCA CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT 1851 CGGCAACCAG ATCATCCCCG TTTAAACGAA TTCGAGCTCG GTACCAGTGA 1901 AGACAACACC ACTAGCACAA ATCGGATCCT GGGAAACAGG CAGAACTTCG 1951 GTTCATAAGC TCGGGTAGGC TGTCAACCTA CCGCCGTATC GTATTGTGTT 2001 TGTCTAGATA CGTATCGCGA GTCGACCTGC AGGCATGCAA GCTGATCCGG 2051 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG 2101 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT 2151 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG 2201 CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC 2251 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT 2301 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA ATGGTTTCTT 2351 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 2401 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 2451 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 2501 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 2551 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 2601 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 2651 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTCAAA 2701 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 2751 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 2801 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 2851 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 2901 AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 2951 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 3001 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 3051 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 3101 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 3151 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 3201 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 3251 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 3301 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAATT 3351 CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC 3401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 3451 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 3501 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 3551 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 3601 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 3651 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 3701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 3751 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 3801 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 3851 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 3901 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA 3951 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 4001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 4051 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT 4101 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 4151 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 4201 TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC CTTCGCTGGG 4251 ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG TGGGGGTAAT 4301 GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT AAAATTTATC 4351 AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT TTTTACCTGT 4401 CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT ACAATTAAGG 4451 GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC 4501 CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG GGATCTTTTC 4551 AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA 4601 TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT 4651 CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG 4701 AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG 4751 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT 4801 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG 4851 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC 4901 AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG 4951 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCAT File updated 7-Nov-08.