pGL4.30[luc2P/NFAT-RE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.30[luc2P/NFAT-RE/Hygro] Vector sequence reference points: Base pairs 6097 NFAT response element (NFAT-RE) 33-122 Minimal promoter 136-166 luc2P reporter gene 228-2003 SV40 late poly(A) region 2043-2264 SV40 early enhancer/promoter 2312-2730 Synthetic hygromycin (Hygr) coding region 2755-3792 Synthetic poly(A) signal 3816-3864 Reporter Vector primer 4 (RVprimer4) binding region 3931-3950 ColE1-derived plasmid replication origin 4188 Synthetic Beta-lactamase (Ampr) coding region 4979-5839 Synthetic poly(A) signal/transcriptional pause site 5944-6097 Reporter Vector primer 3 (RVprimer3) binding region 6046-6065 .. 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGGAGGAAA AACTGTTTCA 51 TACAGAAGGC GTGGAGGAAA AACTGTTTCA TACAGAAGGC GTGGAGGAAA 101 AACTGTTTCA TACAGAAGGC GTAGATCTAG ACTCTAGAGG GTATATAATG 151 GAAGCTCGAA TTCCAGCTTG GCATTCCGGT ACTGTTGGTA AAAAGCTTGG 201 CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA 251 GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC 301 AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC 351 TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA 401 GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA 451 ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC 501 GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT 551 CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG 601 TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG 651 AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA 701 CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG 751 GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC 801 ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT 851 AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC 901 CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG 951 CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG 1001 CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC 1051 GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA 1101 TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA 1151 CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG 1201 AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC 1251 CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA 1301 GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG 1351 ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC 1401 GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC 1451 AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT 1501 ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG 1551 ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT 1601 GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG 1651 ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA 1701 ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC 1751 CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG 1801 GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG 1851 GCCAAGAAGG GCGGCAAGAT CGCCGTGAAT TCTCACGGCT TCCCTCCCGA 1901 GGTGGAGGAG CAGGCCGCCG GCACCCTGCC CATGAGCTGC GCCCAGGAGA 1951 GCGGCATGGA TAGACACCCT GCTGCTTGCG CCAGCGCCAG GATCAACGTC 2001 TAAGGCCGCG ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG 2051 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 2101 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 2151 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 2201 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT 2251 CTACAAATGT GGTAAAATCG ATAAGGATCC GTTTGCGTAT TGGGCGCTCT 2301 TCCGCTGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA 2351 CTTGGTTAGC TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT 2401 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 2451 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 2501 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 2551 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 2601 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 2651 CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 2701 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC 2751 CACCATGAAG AAGCCCGAAC TCACCGCTAC CAGCGTTGAA AAATTTCTCA 2801 TCGAGAAGTT CGACAGTGTG AGCGACCTGA TGCAGTTGTC GGAGGGCGAA 2851 GAGAGCCGAG CCTTCAGCTT CGATGTCGGC GGACGCGGCT ATGTACTGCG 2901 GGTGAATAGC TGCGCTGATG GCTTCTACAA AGACCGCTAC GTGTACCGCC 2951 ACTTCGCCAG CGCTGCACTA CCCATCCCCG AAGTGTTGGA CATCGGCGAG 3001 TTCAGCGAGA GCCTGACATA CTGCATCAGT AGACGCGCCC AAGGCGTTAC 3051 TCTCCAAGAC CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA CAGCCTGTCG 3101 CCGAAGCTAT GGATGCTATT GCCGCCGCCG ACCTCAGTCA AACCAGCGGC 3151 TTCGGCCCAT TCGGGCCCCA AGGCATCGGC CAGTACACAA CCTGGCGGGA 3201 TTTCATTTGC GCCATTGCTG ATCCCCATGT CTACCACTGG CAGACCGTGA 3251 TGGACGACAC CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA CGAACTGATG 3301 CTGTGGGCCG AAGACTGTCC CGAGGTGCGC CACCTCGTCC ATGCCGACTT 3351 CGGCAGCAAC AACGTCCTGA CCGACAACGG CCGCATCACC GCCGTAATCG 3401 ACTGGTCCGA AGCTATGTTC GGGGACAGTC AGTACGAGGT GGCCAACATC 3451 TTCTTCTGGC GGCCCTGGCT GGCTTGCATG GAGCAGCAGA CTCGCTACTT 3501 CGAGCGCCGG CATCCCGAGC TGGCCGGCAG CCCTCGTCTG CGAGCCTACA 3551 TGCTGCGCAT CGGCCTGGAT CAGCTCTACC AGAGCCTCGT GGACGGCAAC 3601 TTCGACGATG CTGCCTGGGC TCAAGGCCGC TGCGATGCCA TCGTCCGCAG 3651 CGGGGCCGGC ACCGTCGGTC GCACACAAAT CGCTCGCCGG AGCGCAGCCG 3701 TATGGACCGA CGGCTGCGTC GAGGTGCTGG CCGACAGCGG CAACCGCCGG 3751 CCCAGTACAC GACCGCGCGC TAAGGAGGTA GGTCGAGTTT AAACTCTAGA 3801 ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT 3851 TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG 3901 CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC 3951 GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC 4001 GGCAGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT 4051 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 4101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 4151 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 4201 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 4251 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 4301 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 4351 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 4401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 4451 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 4501 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 4551 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 4601 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 4651 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 4701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 4751 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 4801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 4851 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 4901 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC 4951 GGCCGCAAAT GCTAAACCAC TGCAGTGGTT ACCAGTGCTT GATCAGTGAG 5001 GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG TGGCCTGACT 5051 CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA 5101 GCGCAGCAAT GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA 5151 GCAATGAACC AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC 5201 TTTGTCCGCC TCCATCCAGT CTATGAGCTG CTGTCGTGAT GCTAGAGTAA 5251 GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT TGCTACTGGC 5301 ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA ACTCTGGTTC 5351 CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG 5401 TCAGCTCCTT AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG 5451 TTGTCGCTCA TGGTAATGGC AGCACTACAC AATTCTCTTA CCGTCATGCC 5501 ATCCGTAAGA TGCTTTTCCG TGACCGGCGA GTACTCAACC AAGTCGTTTT 5551 GTGAGTAGTG TATACGGCGA CCAAGCTGCT CTTGCCCGGC GTCTATACGG 5601 GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA TCATCGGGAA 5651 TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA 5701 GTTCGATATA GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT 5751 TTCACCAGCG TTTCGGGGTG TGCAAAAACA GGCAAGCAAA ATGCCGCAAA 5801 GAAGGGAATG AGTGCGACAC GAAAATGTTG GATGCTCATA CTCGTCCTTT 5851 TTCAATATTA TTGAAGCATT TATCAGGGTT ACTAGTACGT CTCTCAAGGA 5901 TAAGTAAGTA ATATTAAGGT ACGGGAGGTA TTGGACAGGC CGCAATAAAA 5951 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA 6001 GTACTAACAT ACGCTCTCCA TCAAAACAAA ACGAAACAAA ACAAACTAGC 6051 AAAATAGGCT GTCCCCAGTG CAAGTGCAGG TGCCAGAACA TTTCTCT Sequence file prepared 8-Sep-06.