pFN28A HaloTag(R) CMV-neo Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN28A HaloTag(R) CMV-neo Flexi(R) Vector sequence reference points: Base pairs 6532 CMV immediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 HaloTag 7 N terminal region 1067-1957 HaloTag linker 1958-1996 TEV protease recognition region 1970-1990 Sgf I site 1997-2004 Barnase coding region 2028-2363 PmeI region 2365-2372 SV40 late poly(A) region 2524-2745 SV40 enhancer and early promoter 2844-3262 Neomycin phosphotransferase gene 3307-4101 Synthetic polyadenylation region 4165-4213 Beta-lactamase coding region 4474-5334 ColE1-derived plasmid origin of replication 5489-5525 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCCACCATGG CAGAAATCGG TACTGGCTTT CCATTCGACC 1101 CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT 1151 CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC 1201 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA 1251 TTGCTCCAGA CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT 1301 TATTTCTTCG ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT 1351 GGGTCTGGAA GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG 1401 GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT 1451 ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC 1501 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA 1551 TCATCGATCA GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC 1601 CGCCCGCTGA CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA 1651 TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG 1701 CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG 1751 CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT 1801 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT 1851 GCAAGGCTGT GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC 1901 CCGGACCTGA TCGGCAGCGA GATCGCGCGC TGGCTGTCGA CGCTCGAGAT 1951 TTCCGGCGAG CCAACCACTG AGGATCTGTA CTTTCAGAGC GATAACGCGA 2001 TCGCCATGGA ATAAGTAAGG AATCCACATG GCACAGGTTA TCAACACGTT 2051 TGACGGGGTT GCGGATTATC TTCAGACATA TCATAAGCTA CCTGATAATT 2101 ACATTACAAA ATCAGAAGCA CAAGCCCTCG GCTGGGTGGC ATCAAAAGGG 2151 AACCTTGCAG ACGTCGCTCC GGGGAAAAGC ATCGGCGGAG ACATCTTCTC 2201 AAACAGGGAA GGCAAACTCC CGGGCAAAAG CGGACGAACA TGGCGTGAAG 2251 CGGATATTAA CTATACATCA GGCTTCAGAA ATTCAGACCG GATTCTTTAC 2301 TCAAGCGACT GGCTGATTTA CAAAACAACG GACCATTATC AGACCTTTAC 2351 AAAAATCAGA TAATGTTTAA ACGAATTCGG GCTCGGTACC CGGGGATCCT 2401 CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA TCCGGCTGCT AACAAAGCCC 2451 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA 2501 CCCCTTGGGG CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT 2551 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 2601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA 2651 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGA 2701 TGTGGGAGGT TTTTTTAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC 2751 GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCTGATGC GGTATTTTCT 2801 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATACGCGGA TCTGCGCAGC 2851 ACCATGGCCT GAAATAACCT CTGAAAGAGG AACTTGGTTA GGTACCTTCT 2901 GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 2951 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 3001 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 3051 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 3101 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 3151 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT 3201 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 3251 TGCAAAAAGC TTGATTCTTC TGACACAACA GTCTCGAACT TAAGGCTAGA 3301 GCCACCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG 3351 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 3401 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 3451 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC 3501 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG 3551 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 3601 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT 3651 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 3701 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 3751 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT 3801 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG 3851 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 3901 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC 3951 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC 4001 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 4051 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 4101 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 4151 ATCACGATGG CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT 4201 GGTTTTTTGT GTGAATCGAT AGCGATAAGG ATCCTCTTTG CGCTTGCGTT 4251 TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTCAGCACC 4301 GTTTCTGCGG ACTGGCTTTC TACGTAATGG TTTCTTAGAC GTCAGGTGGC 4351 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 4401 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4451 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC 4501 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 4551 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 4601 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 4651 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTCAAAGTTC TGCTATGTGG 4701 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 4751 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG 4801 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 4851 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACT ATCGGAGGAC 4901 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 4951 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 5001 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 5051 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG 5101 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 5151 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 5201 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 5251 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 5301 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAATTCGAA ATGACCGACC 5351 AAGCGACGCC CAACCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 5401 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 5451 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 5501 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 5551 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 5601 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 5651 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 5701 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 5751 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 5801 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 5851 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 5901 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 5951 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 6001 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 6051 CAGATTACGC GCAGAAAAAA AGGATTTCAA GAAGATCCTT TGATCTTTTC 6101 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 6151 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT ATAGTCCGGA 6201 AATACAGGAA CGCACGCTGG ATGGCCCTTC GCTGGGATGG TGAAACCATG 6251 AAAAATGGCA GCTTCAGTGG ATTAAGTGGG GGTAATGTGG CCTGTACCCT 6301 CTGGTTGCAT AGGTATTCAT ACGGTTAAAA TTTATCAGGC GCGATTGCGG 6351 CAGTTTTTCG GGTGGTTTGT TGCCATTTTT ACCTGTCTGC TGCCGTGATC 6401 GCGCTGAACG CGTTTTAGCG GTGCGTACAA TTAAGGGATT ATGGTAAATC 6451 CACTTACTGT CTGCCCTCGT AGCCATCGAG ATAAACCGCA GTACTCCGGC 6501 CACGATGCGT CCGGCGTAGA GGATCGAGAT CT Sequence file prepared 25-June-2011.