pFC30A His6HaloTag® T7 Flexi® Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC30A His6HaloTag® T7 Flexi® Vector sequence reference points: Base pairs 4312 T7 RNA polymerase promoter (-17 to +3) 21-40 SgfI region 61-68 EcoICRI region 447-452 HaloTag® linker region 452-496 TEV protease region 467-487 HaloTag® region 497-1387 His6HaloTag® coding region 497-1405 His6 region 1388-1405 T7 terminator region 1430-1477 Beta-lactamase (AmpR) coding region 1811-2671 ColE1-derived plasmid origin of replication 2826-2862 rrnB transcription terminator 3869-4270 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GCGATCGCCA TGGAATAAGT AAGGAATCCA CATGGCACAG 101 GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA CATATCATAA 151 GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC CTCGGCTGGG 201 TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA AAGCATCGGC 251 GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA AAAGCGGACG 301 AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC AGAAATTCAG 351 ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC AACGGACCAT 401 TATCAGACCT TTACAAAAAT CAGATAATGT TTAATGACCC CGTGTCGAGC 451 TCTCGAGCCA ACCACTGAGG ATCTGTACTT TCAGAGCGAT AACGATGGAT 501 CCGAAATCGG TACTGGCTTT CCATTCGACC CCCATTATGT GGAAGTCCTG 551 GGCGAGCGCA TGCACTACGT CGATGTTGGT CCGCGCGATG GCACCCCTGT 601 GCTGTTCCTG CACGGTAACC CGACCTCCTC CTACGTGTGG CGCAACATCA 651 TCCCGCATGT TGCACCGACC CATCGCTGCA TTGCTCCAGA CCTGATCGGT 701 ATGGGCAAAT CCGACAAACC AGACCTGGGT TATTTCTTCG ACGACCACGT 751 CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA GAGGTCGTCC 801 TGGTCATTCA CGACTGGGGC TCCGCTCTGG GTTTCCACTG GGCCAAGCGC 851 AATCCAGAGC GCGTCAAAGG TATTGCATTT ATGGAGTTCA TCCGCCCTAT 901 CCCGACCTGG GACGAATGGC CAGAATTTGC CCGCGAGACC TTCCAGGCCT 951 TCCGCACCAC CGACGTCGGC CGCAAGCTGA TCATCGATCA GAACGTTTTT 1001 ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC CGCCCGCTGA CTGAAGTCGA 1051 GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC CGCGAGCCAC 1101 TGTGGCGCTT CCCAAACGAG CTGCCAATCG CCGGTGAGCC AGCGAACATC 1151 GTCGCGCTGG TCGAAGAATA CATGGACTGG CTGCACCAGT CCCCTGTCCC 1201 GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT TCTGATCCCA CCGGCCGAAG 1251 CCGCTCGCCT GGCCAAAAGC CTGCCTAACT GCAAGGCTGT GGACATCGGC 1301 CCGGGTCTGA ATCTGCTGCA AGAAGACAAC CCGGACCTGA TCGGCAGCGA 1351 GATCGCGCGC TGGCTGTCTA CTCTGGAGAT TTCCGGTCAT CATCACCATC 1401 ACCACTAATA GTCAACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1451 TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG 1501 TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC CATGTAAGCC 1551 CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG 1601 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT 1651 GGCTTTCTAC GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA 1701 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 1751 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 1801 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 1851 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 1901 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 1951 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 2001 TTCCAATGAT GAGCACTTTC AAAGTTCTGC TATGTGGCGC GGTATTATCC 2051 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 2101 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2151 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 2201 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC 2251 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 2301 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 2351 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 2401 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2451 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 2501 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 2551 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 2601 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 2651 TCACTGATTA AGCATTGGTA ATTCGAAATG ACCGACCAAG CGACGCCCAA 2701 CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 2751 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 2801 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 2851 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 2901 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 2951 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 3001 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 3051 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 3101 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 3151 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 3201 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 3251 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 3301 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 3351 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 3401 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 3451 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 3501 AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT ACAGGAACGC 3551 ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA AATGGCAGCT 3601 TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG GTTGCATAGG 3651 TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 3701 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT 3751 TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG 3801 CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC GATGCGTCCG 3851 GCGTAGAGGA TCGAGATCTT TTCAGCCTGA TACAGATTAA ATCAGAACGC 3901 AGAAGCGGTC TGATAAAACA GAATTTGCCT GGCGGCAGTA GCGCGGTGGT 3951 CCCACCTGAC CCCATGCCGA ACTCAGAAGT GAAACGCCGT AGCGCCGATG 4001 GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG GGAACTGCCA GGCATCAAAT 4051 AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT 4101 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG 4151 AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA 4201 AACTGCCAGG CATCAAATTA AGCAGAAGGC CATCCTGACG GATGGCCTTT 4251 TTGCGTTTCT ACAAACTCTT TTGTTTATTT TTCTAAATAC ATTCAAATAT 4301 GTATCCGCTC AT Sequence file prepared 02-November-2011.