pFC27K HaloTag(R) CMV-neo Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC27K HaloTag(R) CMV-neo Flexi(R) Vector sequence reference points: Base pairs 5527 CMV immediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 Sgf I site 1056-1063 Barnase coding region 1087-1422 EcoICRI region 1442-1447 HaloTag linker 1447-1491 TEV protease recognition region 1462-1482 HaloTag 7 C terminal region 1492-2382 SV40 late poly(A) region 2516-2737 SV40 enhancer and early promoter 2836-3254 EM7 bacterial promoter 3262-3328 Neomycin phosphotransferase gene 3342-4136 Synthetic polyadenylation region 4200-4248 ColE1-derived plasmid origin of replication 4484-4520 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 AGCCAACCAC TGAGGATCTG TACTTTCAGA GCGATAACGA TGGATCCGAA 1501 ATCGGTACTG GCTTTCCATT CGACCCCCAT TATGTGGAAG TCCTGGGCGA 1551 GCGCATGCAC TACGTCGATG TTGGTCCGCG CGATGGCACC CCTGTGCTGT 1601 TCCTGCACGG TAACCCGACC TCCTCCTACG TGTGGCGCAA CATCATCCCG 1651 CATGTTGCAC CGACCCATCG CTGCATTGCT CCAGACCTGA TCGGTATGGG 1701 CAAATCCGAC AAACCAGACC TGGGTTATTT CTTCGACGAC CACGTCCGCT 1751 TCATGGATGC CTTCATCGAA GCCCTGGGTC TGGAAGAGGT CGTCCTGGTC 1801 ATTCACGACT GGGGCTCCGC TCTGGGTTTC CACTGGGCCA AGCGCAATCC 1851 AGAGCGCGTC AAAGGTATTG CATTTATGGA GTTCATCCGC CCTATCCCGA 1901 CCTGGGACGA ATGGCCAGAA TTTGCCCGCG AGACCTTCCA GGCCTTCCGC 1951 ACCACCGACG TCGGCCGCAA GCTGATCATC GATCAGAACG TTTTTATCGA 2001 GGGTACGCTG CCGATGGGTG TCGTCCGCCC GCTGACTGAA GTCGAGATGG 2051 ACCATTACCG CGAGCCGTTC CTGAATCCTG TTGACCGCGA GCCACTGTGG 2101 CGCTTCCCAA ACGAGCTGCC AATCGCCGGT GAGCCAGCGA ACATCGTCGC 2151 GCTGGTCGAA GAATACATGG ACTGGCTGCA CCAGTCCCCT GTCCCGAAGC 2201 TGCTGTTCTG GGGCACCCCA GGCGTTCTGA TCCCACCGGC CGAAGCCGCT 2251 CGCCTGGCCA AAAGCCTGCC TAACTGCAAG GCTGTGGACA TCGGCCCGGG 2301 TCTGAATCTG CTGCAAGAAG ACAACCCGGA CCTGATCGGC AGCGAGATCG 2351 CGCGCTGGCT GTCTACTCTG GAGATTTCCG GTTAATAGAA TTCTAGAGTC 2401 GACCTGCAGG CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA 2451 GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 2501 GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA 2551 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA 2601 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 2651 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG 2701 GTTTTTTTAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA 2751 ACAAAATATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC 2801 ATCTGTGCGG TATTTCACAC CGCATACGCG GATCTGCGCA GCACCATGGC 2851 CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA 2901 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 2951 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 3001 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 3051 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 3101 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 3151 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 3201 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 3251 GCTTAATTAA CTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT 3301 ATAATACGAC AAGGTGAGGA ACTAAACCCA GGAGGCAGAT CATGATTGAA 3351 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 3401 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT 3451 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 3501 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 3551 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 3601 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 3651 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 3701 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG 3751 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 3801 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 3851 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 3901 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 3951 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 4001 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG 4051 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 4101 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 4151 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GATGGCCGCA 4201 ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA 4251 TCGATAGCGA TAAGGATCCT CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT 4301 AGCCCAGTAG CTGACATTCA TCCGGGGTCA GCACCGTTTC TGCGGACTGG 4351 CTTTCTACCC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 4401 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 4451 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 4501 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 4551 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 4601 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 4651 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 4701 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 4751 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 4801 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 4851 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 4901 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 4951 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 5001 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 5051 TACGCGCAGA AAAAAAGGAT TTCAAGAAGA TCCTTTGATC TTTTCTACGG 5101 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 5151 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTATAGT CCGGAAATAC 5201 AGGAACGCAC GCTGGATGGC CCTTCGCTGG GATGGTGAAA CCATGAAAAA 5251 TGGCAGCTTC AGTGGATTAA GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT 5301 TGCATAGGTA TTCATACGGT TAAAATTTAT CAGGCGCGAT TGCGGCAGTT 5351 TTTCGGGTGG TTTGTTGCCA TTTTTACCTG TCTGCTGCCG TGATCGCGCT 5401 GAACGCGTTT TAGCGGTGCG TACAATTAAG GGATTATGGT AAATCCACTT 5451 ACTGTCTGCC CTCGTAGCCA TCGAGATAAA CCGCAGTACT CCGGCCACGA 5501 TGCGTCCGGC GTAGAGGATC GAGATCT Sequence file revised 29-July-2015.