pFC20K (HaloTag(R) 7) T7 SP6 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC20K (HaloTag(R) 7) T7 SP6 Flexi(R) Vector sequence reference points: Base pairs 4417 T7 RNA polymerase promoter (-17 to +3) 21-40 SP6 RNA polymerase promoter (-17 to +3) 45-64 Sgf I site 71-78 Barnase coding region 102-437 EcolCRI site 457-462 TEV site 477-497 HaloTag(R) 7 protein coding region 507-1397 Synthetic poly(A) region 1510-1539 T7 terminator 1540-1587 Kanamycin resistance (Kanr) coding region 1968-2762 ColE1-derived plasmid origin of replication 2931-2967 cer site (site for E. coli XerCD recombinase) 3638-3923 rrnB transcription terminator 3974-4375 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TACGATTTAG 51 GTGACACTAT AGAATAAGGA GCGATCGCCA TGGAATAAGT AAGGAATCCA 101 CATGGCACAG GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA 151 CATATCATAA GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC 201 CTCGGCTGGG TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA 251 AAGCATCGGC GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA 301 AAAGCGGACG AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC 351 AGAAATTCAG ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC 401 AACGGACCAT TATCAGACCT TTACAAAAAT CAGATAATGT TTAATGACCC 451 CGTGTCGAGC TCTCGAGCCA ACCACTGAGG ATCTGTACTT TCAGAGCGAT 501 AACGATGGAT CCGAAATCGG TACTGGCTTT CCATTCGACC CCCATTATGT 551 GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT CCGCGCGATG 601 GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC CTACGTGTGG 651 CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA TTGCTCCAGA 701 CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT TATTTCTTCG 751 ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA 801 GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG GTTTCCACTG 851 GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT ATGGAGTTCA 901 TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC CCGCGAGACC 951 TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA TCATCGATCA 1001 GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC CGCCCGCTGA 1051 CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC 1101 CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG CCGGTGAGCC 1151 AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG CTGCACCAGT 1201 CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT TCTGATCCCA 1251 CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT GCAAGGCTGT 1301 GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC CCGGACCTGA 1351 TCGGCAGCGA GATCGCGCGC TGGCTGTCTA CTCTGGAGAT TTCCGGTTAA 1401 TAGAATTCTA GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC 1451 AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT 1501 AGCATAACCA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAC TAGCATAACC 1551 CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA 1601 CTATATCCGG TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC 1651 CATGTAAGCC CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC 1701 CCTTGTCCAG ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT 1751 TCTGCGGACT GGCTTTCTAC GTGTTCCGCT TCCTTTAGCA GCCCTTGCGC 1801 CCTGAGTGCT TGCGGCAGCG TGAGCTTCAA AAGAATTGCC AGCTGGGGCG 1851 CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT 1901 GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG 1951 GATGACGGTC GTTTCGCATG CTTGAACAAG ATGGATTGCA CGCAGGTTCT 2001 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 2051 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 2101 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 2151 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 2201 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 2251 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 2301 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 2351 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 2401 GCACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 2451 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGTAT 2501 GCCGGATGGT GAGGATCTCG TCGTGACTCA TGGCGATGCC TGCTTGCCGA 2551 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 2601 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 2651 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 2701 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 2751 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 2801 CCCAACCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 2851 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 2901 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 2951 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 3001 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 3051 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 3101 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 3151 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 3201 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 3251 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 3301 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 3351 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 3401 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 3451 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 3501 GCGCAGAAAA AAAGGATTTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 3551 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 3601 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTATAGTCCG GAAATACAGG 3651 AACGCACGCT GGATGGCCCT TCGCTGGGAT GGTGAAACCA TGAAAAATGG 3701 CAGCTTCAGT GGATTAAGTG GGGGTAATGT GGCCTGTACC CTCTGGTTGC 3751 ATAGGTATTC ATACGGTTAA AATTTATCAG GCGCGATTGC GGCAGTTTTT 3801 CGGGTGGTTT GTTGCCATTT TTACCTGTCT GCTGCCGTGA TCGCGCTGAA 3851 CGCGTTTTAG CGGTGCGTAC AATTAAGGGA TTATGGTAAA TCCACTTACT 3901 GTCTGCCCTC GTAGCCATCG AGATAAACCG CAGTACTCCG GCCACGATGC 3951 GTCCGGCGTA GAGGATCGAG ATCTTTTCAG CCTGATACAG ATTAAATCAG 4001 AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG 4051 GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC 4101 CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT 4151 CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG 4201 TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGGGAGCGG 4251 ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG 4301 CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG 4351 CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA AATACATTCA 4401 AATATGTATC CGCTCAT Sequence file prepared 17-Apr-08.