pFC20A (HaloTag(R) 7) T7 SP6 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC20A (HaloTag(R) 7) T7 SP6 Flexi(R) Vector sequence reference points: Base pairs 4422 T7 RNA polymerase promoter (-17 to +3) 21-40 SP6 RNA polymerase promoter (-17 to +3) 45-64 Sgf I site 71-78 Barnase coding region 102-437 EcolCRI site 457-462 TEV site 477-497 HaloTag(R) 7 protein coding region 507-1397 Synthetic poly(A) region 1510-1539 T7 terminator 1540-1587 Beta-lactamase resistance (Ampr) coding region 1921-2781 ColE1-derived plasmid origin of replication 2936-2972 cer site (site for E. coli XerCD recombinase) 3643-3928 rrnB transcription terminator 3979-4380 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TACGATTTAG 51 GTGACACTAT AGAATAAGGA GCGATCGCCA TGGAATAAGT AAGGAATCCA 101 CATGGCACAG GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA 151 CATATCATAA GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC 201 CTCGGCTGGG TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA 251 AAGCATCGGC GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA 301 AAAGCGGACG AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC 351 AGAAATTCAG ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC 401 AACGGACCAT TATCAGACCT TTACAAAAAT CAGATAATGT TTAATGACCC 451 CGTGTCGAGC TCTCGAGCCA ACCACTGAGG ATCTGTACTT TCAGAGCGAT 501 AACGATGGAT CCGAAATCGG TACTGGCTTT CCATTCGACC CCCATTATGT 551 GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT CCGCGCGATG 601 GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC CTACGTGTGG 651 CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA TTGCTCCAGA 701 CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT TATTTCTTCG 751 ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT GGGTCTGGAA 801 GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG GTTTCCACTG 851 GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT ATGGAGTTCA 901 TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC CCGCGAGACC 951 TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA TCATCGATCA 1001 GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC CGCCCGCTGA 1051 CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA TCCTGTTGAC 1101 CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG CCGGTGAGCC 1151 AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG CTGCACCAGT 1201 CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT TCTGATCCCA 1251 CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT GCAAGGCTGT 1301 GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC CCGGACCTGA 1351 TCGGCAGCGA GATCGCGCGC TGGCTGTCTA CTCTGGAGAT TTCCGGTTAA 1401 TAGAATTCTA GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC 1451 AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT 1501 AGCATAACCA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAC TAGCATAACC 1551 CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA 1601 CTATATCCGG TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC 1651 CATGTAAGCC CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC 1701 CCTTGTCCAG ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT 1751 TCTGCGGACT GGCTTTCTAC GTAATGGTTT CTTAGACGTC AGGTGGCACT 1801 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 1851 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 1901 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 1951 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 2001 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 2051 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 2101 GAAGAACGTT TTCCAATGAT GAGCACTTTC AAAGTTCTGC TATGTGGCGC 2151 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2201 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 2251 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 2301 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA 2351 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 2401 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 2451 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2501 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 2551 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 2601 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 2651 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 2701 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 2751 GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG ACCGACCAAG 2801 CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 2851 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 2901 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 2951 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 3001 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 3051 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 3101 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 3151 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 3201 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 3251 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 3301 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 3351 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 3401 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 3451 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 3501 ATTACGCGCA GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC 3551 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 3601 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT 3651 ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA 3701 AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 3751 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG 3801 TTTTTCGGGT GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG 3851 CTGAACGCGT TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC 3901 TTACTGTCTG CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC 3951 GATGCGTCCG GCGTAGAGGA TCGAGATCTT TTCAGCCTGA TACAGATTAA 4001 ATCAGAACGC AGAAGCGGTC TGATAAAACA GAATTTGCCT GGCGGCAGTA 4051 GCGCGGTGGT CCCACCTGAC CCCATGCCGA ACTCAGAAGT GAAACGCCGT 4101 AGCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG GGAACTGCCA 4151 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT 4201 ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG 4251 AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC 4301 GCCCGCCATA AACTGCCAGG CATCAAATTA AGCAGAAGGC CATCCTGACG 4351 GATGGCCTTT TTGCGTTTCT ACAAACTCTT TTGTTTATTT TTCTAAATAC 4401 ATTCAAATAT GTATCCGCTC AT Sequence file prepared 17-Apr-08.