pFC16K (HaloTag(R) 7) CMVd2 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC16K (HaloTag(R) 7) CMVd2 Flexi(R) Vector sequence reference points: Base pairs 4129 CMVd2 intermediate early enhancer/promoter 1-73 T7 RNA polymerase promoter (-17 to +3) 84-103 SP6 RNA polymerase promoter (-17 to +3) 108-127 SgfI region 134-141 barnase coding region 165-500 EcoICRI region 520-525 TEV protease recognition sequence 540-560 HaloTag(R) 7 coding region 570-1460 SV40 late polyadenylation signal 1594-1815 Kanamycin resistance (Kanr) coding region 2123-2917 ColE1-derived plasmid origin of replication 3086-3122 Cer region (site for E. coli XerCD recombinase) 3793-4078 .. 1 GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 51 CTGGTTTAGT GAACCGTCAG ATCACTAGAA GCTTAATACG ACTCACTATA 101 GGGTACGATT TAGGTGACAC TATAGAATAA GGAGCGATCG CCATGGAATA 151 AGTAAGGAAT CCACATGGCA CAGGTTATCA ACACGTTTGA CGGGGTTGCG 201 GATTATCTTC AGACATATCA TAAGCTACCT GATAATTACA TTACAAAATC 251 AGAAGCACAA GCCCTCGGCT GGGTGGCATC AAAAGGGAAC CTTGCAGACG 301 TCGCTCCGGG GAAAAGCATC GGCGGAGACA TCTTCTCAAA CAGGGAAGGC 351 AAACTCCCGG GCAAAAGCGG ACGAACATGG CGTGAAGCGG ATATTAACTA 401 TACATCAGGC TTCAGAAATT CAGACCGGAT TCTTTACTCA AGCGACTGGC 451 TGATTTACAA AACAACGGAC CATTATCAGA CCTTTACAAA AATCAGATAA 501 TGTTTAATGA CCCCGTGTCG AGCTCTCGAG CCAACCACTG AGGATCTGTA 551 CTTTCAGAGC GATAACGATG GATCCGAAAT CGGTACTGGC TTTCCATTCG 601 ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA CGTCGATGTT 651 GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA ACCCGACCTC 701 CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG ACCCATCGCT 751 GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 801 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC 851 CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC 901 TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA AGGTATTGCA 951 TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT GGCCAGAATT 1001 TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC GGCCGCAAGC 1051 TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 1101 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT 1151 GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA 1201 TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA ATACATGGAC 1251 TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG GCACCCCAGG 1301 CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA AGCCTGCCTA 1351 ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 1401 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CTACTCTGGA 1451 GATTTCCGGT TAATAGAATT CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA 1501 TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG 1551 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CGGCCGCTTC GAGCAGACAT 1601 GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 1651 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 1701 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT 1751 GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT TTTTTTAAGC AAGTAAAACC 1801 TCTACAAATG TGGTAAAATC GAATTCTAAT GGATCCTCTT TGCGCTTGCG 1851 TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC GGGGTCAGCA 1901 CCGTTTCTGC GGACTGGCTT TCTACGTGTT CCGCTTCCTT TAGCAGCCCT 1951 TGCGCCCTGA GTGCTTGCGG CAGCGTGAGC TTCAAAAGAA TTGCCAGCTG 2001 GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT 2051 TTCTTGCCGC CAAGGATCTG ATGGCGCAGG GGATCAAGAT CTGATCAAGA 2101 GACAGGATGA CGGTCGTTTC GCATGCTTGA ACAAGATGGA TTGCACGCAG 2151 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 2201 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 2251 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 2301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 2351 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 2401 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 2451 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 2501 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 2551 AGCACGCACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 2601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 2651 CGTATGCCGG ATGGTGAGGA TCTCGTCGTG ACTCATGGCG ATGCCTGCTT 2701 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 2751 GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 2801 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 2851 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 2901 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 2951 CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3001 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 3051 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 3101 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 3151 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 3201 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 3251 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 3301 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 3351 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 3401 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 3451 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 3501 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 3551 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 3601 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 3651 ATTACGCGCA GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC 3701 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 3751 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT 3801 ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA 3851 AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 3901 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG 3951 TTTTTCGGGT GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG 4001 CTGAACGCGT TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC 4051 TTACTGTCTG CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC 4101 GATGCGTCCG GCGTAGAGGA TCGAGATCT Vector sequence prepared 28-Jan-08.