pFN38K HiBiT CMV-neo Flexi Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN38K HiBiT CMV-neo Flexi Vector sequence reference points: Base pairs 4663 CMV immediate-early enhancer/promoter 1-742 chimeric intron 857-989 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 HiBiT 1065-1100 EcoICR I 1103-1108 SgfI Site 1125-1132 Barnase coding region 1156-1491 PmeI site 1493-1500 Sv40 late polyadenylation signal 1652-1873 SV40 enhancer and early promoter 1972-2390 SV40 Enhancer 2045-2281 (Reverse) Sv40 Min Ori 2288-2353 EM7 bacterial promoter 2398-2464 Neo-Kan Resistance 2478-3272 Synthetic PolyAdenylation Signal Sequence 3336-3384 ColE1-derived plasmid replication origin 3620-3656 ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGGTG AGCGGCTGGC GGCTGTTCAA GAAGATTAGC 1101 GGGAGCTCCG GTGGCTCGAG CGGTGCGATC GCCATGGAAT AAGTAAGGAA 1151 TCCACATGGC ACAGGTTATC AACACGTTTG ACGGGGTTGC GGATTATCTT 1201 CAGACATATC ATAAGCTACC TGATAATTAC ATTACAAAAT CAGAAGCACA 1251 AGCCCTCGGC TGGGTGGCAT CAAAAGGGAA CCTTGCAGAC GTCGCTCCGG 1301 GGAAAAGCAT CGGCGGAGAC ATCTTCTCAA ACAGGGAAGG CAAACTCCCG 1351 GGCAAAAGCG GACGAACATG GCGTGAAGCG GATATTAACT ATACATCAGG 1401 CTTCAGAAAT TCAGACCGGA TTCTTTACTC AAGCGACTGG CTGATTTACA 1451 AAACAACGGA CCATTATCAG ACCTTTACAA AAATCAGATA ATGTTTAAAC 1501 GAATTCGGGC TCGGTACCCG GGGATCCTCT AGAGTCGACC TGCAGGCATG 1551 CAAGCTGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC 1601 TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCG GCCGCTTCGA 1651 GCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT 1701 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT 1751 TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT 1801 CATTTTATGT TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTTAAGCAA 1851 GTAAAACCTC TACAAATGTG GTAAAATCGA ATTTTAACAA AATATTAACG 1901 CTTACAATTT CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT 1951 TCACACCGCA TACGCGGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT 2001 GAAAGAGGAA CTTGGTTAGG TACCTTCTGA GGCGGAAAGA ACCAGCTGTG 2051 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 2101 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 2151 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 2201 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 2251 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 2301 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG 2351 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT AATTAACTGT 2401 TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG 2451 TGAGGAACTA AACCCAGGAG GCAGATCATG ATTGAACAAG ATGGATTGCA 2501 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG 2551 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 2601 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 2651 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG 2701 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 2751 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC 2801 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA 2851 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 2901 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 2951 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA 3001 AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 3051 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA 3101 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA 3151 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 3201 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 3251 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA 3301 CCAAGCGACG CCCAACCTGC CATCACGATG GCCGCAATAA AATATCTTTA 3351 TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGCGATAAG 3401 GATCCTCTTT GCGCTTGCGT TTTCCCTTGT CCAGATAGCC CAGTAGCTGA 3451 CATTCATCCG GGGTCAGCAC CGTTTCTGCG GACTGGCTTT CTACCCGGTA 3501 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 3551 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 3601 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 3651 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 3701 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 3751 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA 3801 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 3851 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 3901 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 3951 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 4001 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 4051 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT 4101 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 4151 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 4201 AAGGATTTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 4251 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 4301 GATCTTCACC TAGATCCTTT TATAGTCCGG AAATACAGGA ACGCACGCTG 4351 GATGGCCCTT CGCTGGGATG GTGAAACCAT GAAAAATGGC AGCTTCAGTG 4401 GATTAAGTGG GGGTAATGTG GCCTGTACCC TCTGGTTGCA TAGGTATTCA 4451 TACGGTTAAA ATTTATCAGG CGCGATTGCG GCAGTTTTTC GGGTGGTTTG 4501 TTGCCATTTT TACCTGTCTG CTGCCGTGAT CGCGCTGAAC GCGTTTTAGC 4551 GGTGCGTACA ATTAAGGGAT TATGGTAAAT CCACTTACTG TCTGCCCTCG 4601 TAGCCATCGA GATAAACCGC AGTACTCCGG CCACGATGCG TCCGGCGTAG 4651 AGGATCGAGA TCT Sequence file prepared 8/11/2017 by Feature database and feature finder version 1.0.5.0.