pFN29K His6HaloTag® T7 Flexi® Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN29K His6HaloTag® T7 Flexi® Vector sequence reference points: Base pairs 4393 T7 RNA polymerase promoter (-17 to +3) 21-40 His6HaloTag® coding region 70-981 His6 region 76-93 HaloTag® region 94-981 HaloTag® linker region 982-1020 TEV protease region 994-1014 SgfI region 1021-1028 PmeI region 1389-1396 T7 terminator region 1516-1563 Kanamycin resistance(KanR) coding region 1944-2738 ColE1-derived plasmid origin of replication 2907-2943 rrnB transcription terminator 3950-4351 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GAATTCATCA TGAAACATCA TCACCATCAC CACGCAGAAA 101 TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT CCTGGGCGAG 151 CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT 201 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC 251 ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC 301 AAATCCGACA AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT 351 CATGGATGCC TTCATCGAAG CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA 401 TTCACGACTG GGGCTCCGCT CTGGGTTTCC ACTGGGCCAA GCGCAATCCA 451 GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC CTATCCCGAC 501 CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA 551 CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG 601 GGTACGCTGC CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA 651 CCATTACCGC GAGCCGTTCC TGAATCCTGT TGACCGCGAG CCACTGTGGC 701 GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG AGCCAGCGAA CATCGTCGCG 751 CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG TCCCGAAGCT 801 GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC GAAGCCGCTC 851 GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT 901 CTGAATCTGC TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC 951 GCGCTGGCTG TCGACGCTCG AGATTTCCGG CGAGCCAACC ACTGAGGATC 1001 TGTACTTTCA GAGCGATAAC GCGATCGCCA TGGAATAAGT AAGGAATCCA 1051 CATGGCACAG GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA 1101 CATATCATAA GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC 1151 CTCGGCTGGG TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA 1201 AAGCATCGGC GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA 1251 AAAGCGGACG AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC 1301 AGAAATTCAG ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC 1351 AACGGACCAT TATCAGACCT TTACAAAAAT CAGATAATGT TTAAACGAAT 1401 TCGGGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG 1451 CTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC 1501 ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT 1551 GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGTTCG CTTGCTGTCC 1601 ATAAAACCGC CCAGTCTAGC TATCGCCATG TAAGCCCACT GCAAGCTACC 1651 TGCTTTCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT 1701 GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTGT 1751 TCCGCTTCCT TTAGCAGCCC TTGCGCCCTG AGTGCTTGCG GCAGCGTGAG 1801 CTTCAAAAGA ATTGCCAGCT GGGGCGCCCT CTGGTAAGGT TGGGAAGCCC 1851 TGCAAAGTAA ACTGGATGGC TTTCTTGCCG CCAAGGATCT GATGGCGCAG 1901 GGGATCAAGA TCTGATCAAG AGACAGGATG ACGGTCGTTT CGCATGCTTG 1951 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 2001 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 2051 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 2101 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 2151 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 2201 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC 2251 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 2301 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 2351 AGCGAAACAT CGCATCGAGC GAGCACGCAC TCGGATGGAA GCCGGTCTTG 2401 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 2451 CTGTTCGCCA GGCTCAAGGC GCGTATGCCG GATGGTGAGG ATCTCGTCGT 2501 GACTCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT 2551 TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 2601 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 2651 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 2701 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 2751 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCGGTATCA GCTCACTCAA 2801 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 2851 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 2901 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 2951 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 3001 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 3051 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 3101 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 3151 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 3201 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 3251 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 3301 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 3351 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 3401 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 3451 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATTTCAAGA 3501 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 3551 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 3601 ATCCTTTTAT AGTCCGGAAA TACAGGAACG CACGCTGGAT GGCCCTTCGC 3651 TGGGATGGTG AAACCATGAA AAATGGCAGC TTCAGTGGAT TAAGTGGGGG 3701 TAATGTGGCC TGTACCCTCT GGTTGCATAG GTATTCATAC GGTTAAAATT 3751 TATCAGGCGC GATTGCGGCA GTTTTTCGGG TGGTTTGTTG CCATTTTTAC 3801 CTGTCTGCTG CCGTGATCGC GCTGAACGCG TTTTAGCGGT GCGTACAATT 3851 AAGGGATTAT GGTAAATCCA CTTACTGTCT GCCCTCGTAG CCATCGAGAT 3901 AAACCGCAGT ACTCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT 3951 TTTCAGCCTG ATACAGATTA AATCAGAACG CAGAAGCGGT CTGATAAAAC 4001 AGAATTTGCC TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG 4051 AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG GGTCTCCCCA 4101 TGCGAGAGTA GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG 4151 AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT 4201 GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC 4251 CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GCATCAAATT 4301 AAGCAGAAGG CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT 4351 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CAT Sequence file prepared 02-November-2011.