pNL2.3[secNluc/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNL2.3[secNluc/Hygro] Vector sequence reference points: Base pairs 4793 Multiple cloning region 1-70 Nanoluc IL6 (secNluc) reporter gene 100-699 SV40 late poly(A) region 739-960 SV40 early enhancer/promoter 1008-1426 Synthetic hygromycin (HygR) coding region 1451-2488 Reporter vector primer 4 (RVPrimer4) binding region 2627-2646 Synthetic beta-lactamase (Ampr)coding region 3675-4535 Synthetic poly(A) signal sequence 2512-2560 ColE1-derived plasmid replication origin 2884 Synthetic polyadenylation signal/ transcriptional pause region 4640-4793 Reporter vector primer 3 (RVPrimer3) binding region 4742-4761 .. 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA 101 TGAACTCCTT CTCCACAAGC GCCTTCGGTC CAGTTGCCTT CTCCCTGGGC 151 CTGCTCCTGG TGTTGCCTGC TGCCTTCCCT GCCCCAGTCT TCACACTCGA 201 AGATTTCGTT GGGGACTGGC GACAGACAGC CGGCTACAAC CTGGACCAAG 251 TCCTTGAACA GGGAGGTGTG TCCAGTTTGT TTCAGAATCT CGGGGTGTCC 301 GTAACTCCGA TCCAAAGGAT TGTCCTGAGC GGTGAAAATG GGCTGAAGAT 351 CGACATCCAT GTCATCATCC CGTATGAAGG TCTGAGCGGC GACCAAATGG 401 GCCAGATCGA AAAAATTTTT AAGGTGGTGT ACCCTGTGGA TGATCATCAC 451 TTTAAGGTGA TCCTGCACTA TGGCACACTG GTAATCGACG GGGTTACGCC 501 GAACATGATC GACTATTTCG GACGGCCGTA TGAAGGCATC GCCGTGTTCG 551 ACGGCAAAAA GATCACTGTA ACAGGGACCC TGTGGAACGG CAACAAAATT 601 ATCGACGAGC GCCTGATCAA CCCCGACGGC TCCCTGCTGT TCCGAGTAAC 651 CATCAACGGA GTGACCGGCT GGCGGCTGTG CGAACGCATT CTGGCGTAAG 701 GCCGCGACTC TAGAGTCGGG GCGGCCGGCC GCTTCGAGCA GACATGATAA 751 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA 801 TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT 851 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC 901 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC 951 AAATGTGGTA AAATCGATAA GGATCCGTTT GCGTATTGGG CGCTCTTCCG 1001 CTGATCTGCG CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG 1051 GTTAGCTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT 1101 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 1151 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC 1201 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 1251 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 1301 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 1351 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 1401 GAGGCCTAGG CTTTTGCAAA AAGCTCGATT CTTCTGACAC TAGCGCCACC 1451 ATGAAGAAGC CCGAACTCAC CGCTACCAGC GTTGAAAAAT TTCTCATCGA 1501 GAAGTTCGAC AGTGTGAGCG ACCTGATGCA GTTGTCGGAG GGCGAAGAGA 1551 GCCGAGCCTT CAGCTTCGAT GTCGGCGGAC GCGGCTATGT ACTGCGGGTG 1601 AATAGCTGCG CTGATGGCTT CTACAAAGAC CGCTACGTGT ACCGCCACTT 1651 CGCCAGCGCT GCACTACCCA TCCCCGAAGT GTTGGACATC GGCGAGTTCA 1701 GCGAGAGCCT GACATACTGC ATCAGTAGAC GCGCCCAAGG CGTTACTCTC 1751 CAAGACCTCC CCGAAACAGA GCTGCCTGCT GTGTTACAGC CTGTCGCCGA 1801 AGCTATGGAT GCTATTGCCG CCGCCGACCT CAGTCAAACC AGCGGCTTCG 1851 GCCCATTCGG GCCCCAAGGC ATCGGCCAGT ACACAACCTG GCGGGATTTC 1901 ATTTGCGCCA TTGCTGATCC CCATGTCTAC CACTGGCAGA CCGTGATGGA 1951 CGACACCGTG TCCGCCAGCG TAGCTCAAGC CCTGGACGAA CTGATGCTGT 2001 GGGCCGAAGA CTGTCCCGAG GTGCGCCACC TCGTCCATGC CGACTTCGGC 2051 AGCAACAACG TCCTGACCGA CAACGGCCGC ATCACCGCCG TAATCGACTG 2101 GTCCGAAGCT ATGTTCGGGG ACAGTCAGTA CGAGGTGGCC AACATCTTCT 2151 TCTGGCGGCC CTGGCTGGCT TGCATGGAGC AGCAGACTCG CTACTTCGAG 2201 CGCCGGCATC CCGAGCTGGC CGGCAGCCCT CGTCTGCGAG CCTACATGCT 2251 GCGCATCGGC CTGGATCAGC TCTACCAGAG CCTCGTGGAC GGCAACTTCG 2301 ACGATGCTGC CTGGGCTCAA GGCCGCTGCG ATGCCATCGT CCGCAGCGGG 2351 GCCGGCACCG TCGGTCGCAC ACAAATCGCT CGCCGGAGCG CAGCCGTATG 2401 GACCGACGGC TGCGTCGAGG TGCTGGCCGA CAGCGGCAAC CGCCGGCCCA 2451 GTACACGACC GCGCGCTAAG GAGGTAGGTC GAGTTTAAAC TCTAGAACCG 2501 GTCATGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT 2551 TTTTTGTGTG TTCGAACTAG ATGCTGTCGA CCGATGCCCT TGAGAGCCTT 2601 CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG 2651 CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA 2701 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 2751 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 2801 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 2851 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 2901 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 2951 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 3001 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 3051 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 3101 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 3151 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 3201 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 3251 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 3301 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 3351 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 3401 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 3451 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 3501 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 3551 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 3601 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGCGGCC 3651 GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC AGTGAGGCAC 3701 CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC 3751 GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC 3801 AGCAATGATG CCGCGAGAGC CGCGTTCACC GGCCCCCGAT TTGTCAGCAA 3851 TGAACCAGCC AGCAGGGAGG GCCGAGCGAA GAAGTGGTCC TGCTACTTTG 3901 TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA GAGTAAGAAG 3951 TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT ACTGGCATCG 4001 TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG 4051 CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG 4101 CTCCTTAGGG CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT 4151 CGCTCATGGT AATGGCAGCA CTACACAATT CTCTTACCGT CATGCCATCC 4201 GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT CGTTTTGTGA 4251 GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT ATACGGGACA 4301 ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT 4351 TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC 4401 GATATAGCCC ACTCTTGCAC CCAGTTGATC TTCAGCATCT TTTACTTTCA 4451 CCAGCGTTTC GGGGTGTGCA AAAACAGGCA AGCAAAATGC CGCAAAGAAG 4501 GGAATGAGTG CGACACGAAA ATGTTGGATG CTCATACTCG TCCTTTTTCA 4551 ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT CAAGGATAAG 4601 TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC 4651 TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC 4701 TAACATACGC TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA 4751 TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC AGAACATTTC TCT Sequence file prepared 30-January-2012.