pNL2.2[NlucP/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNL2.2[NlucP/Hygro] Vector sequence reference points: Base pairs 4832 Multiple cloning region 1-70 Nanoluc PEST (NlucP) reporter gene 100-738 SV40 late poly(A) region 778-999 SV40 early enhancer/promoter 1047-1465 Synthetic hygromycin (HygR) coding region 1490-2527 Synthetic poly(A) region 2551-2599 Reporter vector primer 4 (RVPrimer4) binding region 2666-2685 ColE1-derived plasmid replication origin 2923 Synthetic beta-lactamase (Ampr)coding region 3714-4574 Synthetic polyadenylation signal/transcriptional pause region 4679-4832 Reporter vector primer 3 (RVPrimer3) binding region 4781-4800 .. 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA 101 TGGTCTTCAC ACTCGAAGAT TTCGTTGGGG ACTGGCGACA GACAGCCGGC 151 TACAACCTGG ACCAAGTCCT TGAACAGGGA GGTGTGTCCA GTTTGTTTCA 201 GAATCTCGGG GTGTCCGTAA CTCCGATCCA AAGGATTGTC CTGAGCGGTG 251 AAAATGGGCT GAAGATCGAC ATCCATGTCA TCATCCCGTA TGAAGGTCTG 301 AGCGGCGACC AAATGGGCCA GATCGAAAAA ATTTTTAAGG TGGTGTACCC 351 TGTGGATGAT CATCACTTTA AGGTGATCCT GCACTATGGC ACACTGGTAA 401 TCGACGGGGT TACGCCGAAC ATGATCGACT ATTTCGGACG GCCGTATGAA 451 GGCATCGCCG TGTTCGACGG CAAAAAGATC ACTGTAACAG GGACCCTGTG 501 GAACGGCAAC AAAATTATCG ACGAGCGCCT GATCAACCCC GACGGCTCCC 551 TGCTGTTCCG AGTAACCATC AACGGAGTGA CCGGCTGGCG GCTGTGCGAA 601 CGCATTCTGG CGAATTCTCA CGGCTTTCCG CCTGAGGTTG AAGAGCAAGC 651 CGCCGGTACA TTGCCTATGT CCTGCGCACA AGAAAGCGGT ATGGACCGGC 701 ACCCAGCCGC TTGTGCTTCA GCTCGCATCA ACGTCTAAGG CCGCGACTCT 751 AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT 801 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG 851 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA 901 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG 951 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA 1001 AATCGATAAG GATCCGTTTG CGTATTGGGC GCTCTTCCGC TGATCTGCGC 1051 AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGCTACCT 1101 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 1151 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 1201 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 1251 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 1301 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 1351 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG 1401 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 1451 TTTTGCAAAA AGCTCGATTC TTCTGACACT AGCGCCACCA TGAAGAAGCC 1501 CGAACTCACC GCTACCAGCG TTGAAAAATT TCTCATCGAG AAGTTCGACA 1551 GTGTGAGCGA CCTGATGCAG TTGTCGGAGG GCGAAGAGAG CCGAGCCTTC 1601 AGCTTCGATG TCGGCGGACG CGGCTATGTA CTGCGGGTGA ATAGCTGCGC 1651 TGATGGCTTC TACAAAGACC GCTACGTGTA CCGCCACTTC GCCAGCGCTG 1701 CACTACCCAT CCCCGAAGTG TTGGACATCG GCGAGTTCAG CGAGAGCCTG 1751 ACATACTGCA TCAGTAGACG CGCCCAAGGC GTTACTCTCC AAGACCTCCC 1801 CGAAACAGAG CTGCCTGCTG TGTTACAGCC TGTCGCCGAA GCTATGGATG 1851 CTATTGCCGC CGCCGACCTC AGTCAAACCA GCGGCTTCGG CCCATTCGGG 1901 CCCCAAGGCA TCGGCCAGTA CACAACCTGG CGGGATTTCA TTTGCGCCAT 1951 TGCTGATCCC CATGTCTACC ACTGGCAGAC CGTGATGGAC GACACCGTGT 2001 CCGCCAGCGT AGCTCAAGCC CTGGACGAAC TGATGCTGTG GGCCGAAGAC 2051 TGTCCCGAGG TGCGCCACCT CGTCCATGCC GACTTCGGCA GCAACAACGT 2101 CCTGACCGAC AACGGCCGCA TCACCGCCGT AATCGACTGG TCCGAAGCTA 2151 TGTTCGGGGA CAGTCAGTAC GAGGTGGCCA ACATCTTCTT CTGGCGGCCC 2201 TGGCTGGCTT GCATGGAGCA GCAGACTCGC TACTTCGAGC GCCGGCATCC 2251 CGAGCTGGCC GGCAGCCCTC GTCTGCGAGC CTACATGCTG CGCATCGGCC 2301 TGGATCAGCT CTACCAGAGC CTCGTGGACG GCAACTTCGA CGATGCTGCC 2351 TGGGCTCAAG GCCGCTGCGA TGCCATCGTC CGCAGCGGGG CCGGCACCGT 2401 CGGTCGCACA CAAATCGCTC GCCGGAGCGC AGCCGTATGG ACCGACGGCT 2451 GCGTCGAGGT GCTGGCCGAC AGCGGCAACC GCCGGCCCAG TACACGACCG 2501 CGCGCTAAGG AGGTAGGTCG AGTTTAAACT CTAGAACCGG TCATGGCCGC 2551 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGT 2601 TCGAACTAGA TGCTGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA 2651 GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT 2701 GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTTCCG 2751 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 2801 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 2851 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 2901 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 2951 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 3001 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 3051 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 3101 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 3151 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 3201 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 3251 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 3301 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 3351 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 3401 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 3451 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 3501 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 3551 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 3601 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 3651 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG CAAATGCTAA 3701 ACCACTGCAG TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG 3751 ATCTGCCTAT TTCGTTCGTC CATAGTGGCC TGACTCCCCG TCGTGTAGAT 3801 CACTACGATT CGTGAGGGCT TACCATCAGG CCCCAGCGCA GCAATGATGC 3851 CGCGAGAGCC GCGTTCACCG GCCCCCGATT TGTCAGCAAT GAACCAGCCA 3901 GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT GCTACTTTGT CCGCCTCCAT 3951 CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT TCGCCAGTGA 4001 GTAGTTTCCG AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC 4051 TCGTCGTTCG GTATGGCTTC GTTCAACTCT GGTTCCCAGC GGTCAAGCCG 4101 GGTCACATGA TCACCCATAT TATGAAGAAA TGCAGTCAGC TCCTTAGGGC 4151 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CGGTGTTGTC GCTCATGGTA 4201 ATGGCAGCAC TACACAATTC TCTTACCGTC ATGCCATCCG TAAGATGCTT 4251 TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG TAGTGTATAC 4301 GGCGACCAAG CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA 4351 CATAGCAGTA CTTTGAAAGT GCTCATCATC GGGAATCGTT CTTCGGGGCG 4401 GAAAGACTCA AGGATCTTGC CGCTATTGAG ATCCAGTTCG ATATAGCCCA 4451 CTCTTGCACC CAGTTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCG 4501 GGGTGTGCAA AAACAGGCAA GCAAAATGCC GCAAAGAAGG GAATGAGTGC 4551 GACACGAAAA TGTTGGATGC TCATACTCGT CCTTTTTCAA TATTATTGAA 4601 GCATTTATCA GGGTTACTAG TACGTCTCTC AAGGATAAGT AAGTAATATT 4651 AAGGTACGGG AGGTATTGGA CAGGCCGCAA TAAAATATCT TTATTTTCAT 4701 TACATCTGTG TGTTGGTTTT TTGTGTGAAT CGATAGTACT AACATACGCT 4751 CTCCATCAAA ACAAAACGAA ACAAAACAAA CTAGCAAAAT AGGCTGTCCC 4801 CAGTGCAAGT GCAGGTGCCA GAACATTTCT CT Sequence file revised 06-November-2014.