psiSTRIKE(TM) Hygromycin Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 psiSTRIKE(TM) Hygromycin Vector Sequence Reference Points: Base pairs 4792 T7 RNA polymerase transcription initiation site 1 U6 promoter (Human -260 to 0) 16-275 U6 transcription initiation site 276 SP6 RNA polymerase promoter (-17 to +3) 330-349 SP6 RNA polymerase transcription initiation site 332 Binding region of pUC/M13 Reverse Sequencing Primer 367-388 SV40 enhancer and early promoter 601-1019 SV40 minimum origin of replication 917-982 Hygromycin phosphotransferase coding region 1054-2079 Synthetic polyadenylation signal 2114-2162 ColE1-derived plasmid origin of replication 2352 Beta-lactamase (Ampr) coding region 3114-3974 Phage f1 region 4157-4612 Binding region of pUC/M13 Forward Sequencing Primer 4726-4749 T7 RNA polymerase promoter (-17 to +3) 4776-3 .. 1 GGGCGAATTG GGCCCTCGGG CAGGAAGAGG GCCTATTTCC CATGATTCCT 51 TCATATTTGC ATATACGATA CAAGGCTGTT AGAGAGATAA TTAGAATTAA 101 TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA CGTAGAAAGT 151 AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC 201 TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 251 TATCTTGTGG AAAGGACGAA ACACCGTGCA GGAGACGAGA TCTCCGAGCT 301 CCCAACGCGT TGGATGCATA GCTTGAGTAT TCTATAGTGT CACCTAAATA 351 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 401 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 451 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 501 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 551 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTGATCT 601 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT 651 ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG 701 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 751 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 801 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC 851 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 901 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 951 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 1001 AGGCTTTTGC AAAAAGCTTG ATTCTTCTGA CGCTAGCGAT CGCCCGGGCC 1051 ACCATGAAAA AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT 1101 CGAAAAGTTC GACAGCGTGT CCGACCTGAT GCAGCTCTCG GAGGGCGAAG 1151 AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG 1201 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA 1251 CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT 1301 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG 1351 TTGCAAGACC TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC 1401 GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT 1451 TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT 1501 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT 1551 GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC 1601 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC 1651 GGCTCCAACA ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA 1701 CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC GCCAACATCT 1751 TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC 1801 GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC GGGCGTATAT 1851 GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT 1901 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC 1951 GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT 2001 CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC 2051 CCAGCACTCG TCCGAGGGCA AAGGAATAGC TCGAGTTTAA ACTCTAGAAC 2101 CGGTCATGGC CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG 2151 GTTTTTTGTG TGTTCGAACT AGAAGCTTGC TTCCTCGCTC ACTGACTCGC 2201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 2251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 2301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 2351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 2401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 2451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 2501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 2551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 2601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 2651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 2701 GCAGCAGCCA CTGGTAACGG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 2751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 2801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 2851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 2901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 2951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 3001 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 3051 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 3101 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 3151 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 3201 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 3251 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 3301 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 3351 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 3401 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 3451 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 3501 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 3551 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 3601 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 3651 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 3701 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 3751 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 3801 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 3851 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 3901 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 3951 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 4001 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 4051 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 4101 ACCTGATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 4151 ATCAGGAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT 4201 TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC 4251 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT 4301 GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA 4351 AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC 4401 AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG 4451 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA 4501 AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT 4551 AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC 4601 TACAGGGCGC GTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC 4651 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT 4701 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 4751 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TA Sequence file prepared 30-Dec-03.