pGEMEX®-2 Vector Sequence(a,b) This vector can be obtained from Promega Corporation, Madison WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGEMEX®-2 Vector sequence reference points: Base pairs 3995 SP6 RNA polymerase transcription initiation site 1 Multiple cloning region 9-85 T3 RNA polymerase promoter (-17 to +3) 89-108 T3 RNA polymerase transcription initation site 91 T7 gene 10 start codon 907 T7 RNA polymerase promoter (-17 to +3) 968-987 T7 RNA polymerase transcription initiation site 970 Beta-lactamase (Ampr)coding region 2009-2869 Phage f1 region 3306-3761 T7 terminator region 3841-3931 SP6 RNA polymerase promoter (-17 to +3) 3979-3 (a)Usage restrictions apply to Bacterial Strains JM109(DE3) and BL21(DE3)pLysS, to the following Promega products that include these bacterial strains (pGEMEX®-1 and pGEMEX®-2 Vectors) and to any derivatives thereof. Please read the following statement describing these restrictions before purchasing any of these products. Usage Restrictions for the T7 Expression System The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents and patent applications assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused. Academic and NonProfit Laboratories No materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3) and BL21(DE3)pLysS, to the following Promega products that include these bacterial strains (pGEMEX®-1 and pGEMEX®-2 Vectors) and to any derivatives thereof. Commercial Laboratories A license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, FAX: 631-344-3729. (b)U.S. Pat. No. 4,766,072 has been issued to Promega Corporation for transcription vectors having two different bacteriophage RNA polymerase promoter sequences separated by a series of unique restriction sites into which foreign DNA can be inserted. Note: The sequence for pGEMEX®-2 Vector is identical to that of pGEMEX®-1 Vector except for the addition of two cytosine bases at positions 65 and 84 (in relation to the numbering and sequence below) in the pGEMEX®-2 Vector. pGEMEX is a trademark of Promega Corporation and is registered with the U.S. Patent and Trademark Office. .. 1 GAATACTCAA GCTTATGCAT GCGGCCGCAT CTAGAGGGCC CGGATCCCTC 51 GAGGTCGACG AATTCCGAGC TCGGCCGACT TGGCCCTTCC CTTTAGTGAG 101 GGTTAATAAA CTTGGCCAAT TCGATCCGGT ACCAGCACCA CCAGCGGTGA 151 GGTGCGGAAC TTCTACAACC TCAAAGCCCA TAACGTTGCG GATAGAACCC 201 TTCTCAGGGT CAATCAGAGC AGCGTAGTTT GCTGCGTTCG GCATCAGTGC 251 TGCCAGAATC GCAGAGTAGC TATCTGGGTC ACAGTAGAAC ACACGGTCAG 301 CAGCCGGAAC ATAGTTCTTG GTCAGAGCCG CACGAGCCTT AGTCAGAGCC 351 GCAATAATCT CCTTACCCAG CGCAACTTGG TCGGTAAGTG CGGCCTTGTT 401 CTGAGTGGTC TCAATTACGG TAGCAGTACC TAAGCCCTCG ATGTTCTCAT 451 TATATTTGCT TTCCACGTTA CACAGACCGG CAATCTCAGC CAGAACCGCA 501 CCATCCGCAG CCATCGCCAG AGATTCACCC AACTGAGAGG TATACTCAGA 551 GCGAACGTCG TAGTGGTTCA TCGCGTCCTC AATATCATAA ATCAGAACGT 601 CAGCCGTCAG GAGACCGTCA ATGGTGATTA CCTTCTCGGT GTGTTTGATG 651 TCCTTACGTT TATCGTCGAG GTTCTCGCCC GGAGCCAGAT ACGCTGCCTG 701 AGTGCGACCC AGAACAGGGA ACTGAGCGGA TTTACCGCTG GAGATGGAAC 751 GTACCATGTG GCGAGAAGTG GTCACGGAGG TACGAGCGAA CGCAGTCAGG 801 ACTTCACCGC CAAATACCTT CAAGAACAAC GCCAGTTTAT CTCCAGCAGC 851 AACTACACCT TTACCTTGGT TAGTACCCAT TTGCTGTCCA CCAGTCATGC 901 TAGCCATATG TATATCTCCT TCTTAAAGTT AAACAAAATT ATTTCTAGAG 951 GGAAACCGTT GTGGTCTCCC TATAGTGAGT CGTATTAATT TCGCGGGATC 1001 GAGATCTCCC GGGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 1051 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC 1101 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 1151 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 1201 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 1251 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 1301 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 1351 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 1401 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 1451 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 1501 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 1551 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1601 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 1651 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 1701 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 1751 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 1801 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 1851 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 1901 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 1951 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 2001 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 2051 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 2101 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 2151 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 2201 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 2251 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 2301 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 2351 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 2401 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 2451 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 2501 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 2551 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2601 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 2651 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 2701 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 2751 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 2801 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 2851 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 2901 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 2951 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 3001 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT 3051 ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 3101 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG 3151 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 3201 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA 3251 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 3301 TCAGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 3351 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 3401 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 3451 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 3501 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 3551 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 3601 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 3651 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 3701 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 3751 GCTTACAATT TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG 3801 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GGTTATCCGG ATATAGTTCC 3851 TCCTTTCAGC AAAAAACCCC TCAAGACCCG TTTAGAGGCC CCAAGGGGTT 3901 ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG 3951 GCTTTGTTAG CAGCCGGATC AAGCTGGGAT TTAGGTGACA CTATA Sequence updated 11-Jun-01.