pGEMŪ*-2 vector sequence Note: Genetic elements of Promega's plasmid vectors are quality tested for their functionality. The sequence of the multiple cloning site is confirmed using restriction enzyme digestion, but the identity of every base pair of the plasmid has not been established by DNA sequencing. As a result, minor errors may exist in the sequence information provided. Call one of the following numbers for technical information. Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGEMŪ-2 vector sequence reference points: 1. 2869 base pairs 2. SP6 transcription initiation site 1 3. T7 transcription initiation site 70 4. SP6 promoter 2852-6 5. T7 promoter 65-87 7. multiple cloning sites 10-60 8. lac operon sequences 98-156 9. Beta-lactamase coding region 1098-1958 pGEM is a registered trademark of Promega Corporation, Madison, WI. *U.S. Pat. No. 4,766,072 has been issued to Promega Corporation for transcription vectors having two different bacteriophage RNA polymerase promoter sequences separated by a series of unique restriction sites into which foreign DNA can be inserted. .. 1 GAATACACGG AATTCGAGCT CGCCCGGGGA TCCTCTAGAG TCGACCTGCA 51 GCCCAAGCTT CCGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC 101 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 151 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 201 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 251 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 301 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 351 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 401 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 451 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 501 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 551 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 601 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 651 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 701 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 751 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 801 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 851 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 901 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 951 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 1001 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1051 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 1101 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 1151 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG 1201 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 1251 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 1301 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 1351 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 1401 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 1451 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC 1501 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 1551 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 1601 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 1651 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 1701 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 1751 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC 1801 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 1851 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 1901 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 1951 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 2001 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 2051 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA 2101 ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC 2151 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 2201 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 2251 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 2301 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATCGACG 2351 CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA 2401 GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG 2451 CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA 2501 AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT 2551 CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC 2601 ACGATGCGTC CGGCGTAGAG GATCTGGCTA GCGATGACCC TGCTGATTGG 2651 TTCGCTGACC ATTTCCGGGG TGCGGAACGG CGTTACCAGA AACTCAGAAG 2701 GTTCGTCCAA CCAAACCGAC TCTGACGGCA GTTTACGAGA GAGATGATAG 2751 GGTCTGCTTC AGTAAGCCAG ATGCTACACA ATTAGGCTTG TACATATTGT 2801 CGTTAGAACG CGGCTACAAT TAATACATAA CCTTATGTAT CATACACATA 2851 CGATTTAGGT GACACTATA