pFC8K (HaloTag(TM)) CMV Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC8K (HaloTag(TM)) CMV Flexi(R) Vector sequence reference points: Base pairs 5039 CMV intermediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 Sgf I site 1056-1063 barnase coding region 1087-1422 EcoICR I site 1442-1447 Factor Xa linker 1447-1500 HaloTag(TM) coding region 1501-2379 SV40 late polyadenylation signal 2504-2725 Kanamycin resistance coding region 3033-3827 ColE1-derived plasmid origin of replication 3996-4032 cer site (site for E. coli XerCD recombinase) 4703-4988 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 ATCTGATCGA AGGTCGTGGT ATCCCTCGTA ACTCTCGTGT TGATGCGGGA 1501 TCCGAAATCG GTACAGGCTT CCCCTTCGAC CCCCATTATG TGGAAGTCCT 1551 GGGCGAGCGT ATGCACTACG TCGATGTTGG ACCGCGGGAT GGCACGCCTG 1601 TGCTGTTCCT GCACGGTAAC CCGACCTCGT CCTACCTGTG GCGCAACATC 1651 ATCCCGCATG TAGCACCGAG TCATCGGTGC ATTGCTCCAG ACCTGATCGG 1701 GATGGGAAAA TCGGACAAAC CAGACCTCGA TTATTTCTTC GACGACCACG 1751 TCCGCTACCT CGATGCCTTC ATCGAAGCCT TGGGTTTGGA AGAGGTCGTC 1801 CTGGTCATCC ACGACTGGGG CTCAGCTCTC GGATTCCACT GGGCCAAGCG 1851 CAATCCGGAA CGGGTCAAAG GTATTGCATG TATGGAATTC ATCCGGCCTA 1901 TCCCGACGTG GGACGAATGG CCAGAATTCG CCCGTGAGAC CTTCCAGGCC 1951 TTCCGGACCG CCGACGTCGG CCGAGAGTTG ATCATCGATC AGAACGCTTT 2001 CATCGAGGGT GCGCTCCCGA TGGGGGTCGT CCGTCCGCTT ACGGAGGTCG 2051 AGATGGACCA CTATCGCGAG CCCTTCCTCA AGCCTGTTGA CCGAGAGCCA 2101 CTGTGGCGAT TCCCCAACGA GCTGCCCATC GCCGGTGAGC CCGCGAACAT 2151 CGTCGCGCTC GTCGAGGCAT ACATGAACTG GCTGCACCAG TCACCTGTCC 2201 CGAAGTTGTT GTTCTGGGGC ACACCCGGCG TACTGATCCC CCCGGCCGAA 2251 GCCGCGAGAC TTGCCGAAAG CCTCCCCAAC TGCAAGACAG TGGACATCGG 2301 CCCGGGATTG TTCTTGCTCC AGGAAGACAA CCCGGACCTT ATCGGCAGTG 2351 AGATCGCGCG CTGGCTCCCG GCACTCTAAT CTAGAGTCGA CCTGCAGGCA 2401 TGCAAGCTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT 2451 GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CGGCCGCTTC 2501 GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 2551 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT 2601 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 2651 TTCATTTTAT GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT TTTTTTAAGC 2701 AAGTAAAACC TCTACAAATG TGGTAAAATC GAATTCTAAT GGATCCTCTT 2751 TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC 2801 GGGGTCAGCA CCGTTTCTGC GGACTGGCTT TCTACGTGTT CCGCTTCCTT 2851 TAGCAGCCCT TGCGCCCTGA GTGCTTGCGG CAGCGTGAGC TTCAAAAGAA 2901 TTGCCAGCTG GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA 2951 CTGGATGGCT TTCTTGCCGC CAAGGATCTG ATGGCGCAGG GGATCAAGAT 3001 CTGATCAAGA GACAGGATGA CGGTCGTTTC GCATGCTTGA ACAAGATGGA 3051 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 3101 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 3151 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 3201 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC 3251 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 3301 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 3351 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT 3401 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 3451 GCATCGAGCG AGCACGCACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 3501 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG 3551 GCTCAAGGCG CGTATGCCGG ATGGTGAGGA TCTCGTCGTG ACTCATGGCG 3601 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 3651 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT 3701 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT 3751 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 3801 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG 3851 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA 3901 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 3951 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 4001 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 4051 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 4101 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4151 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 4201 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 4251 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 4301 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 4351 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 4401 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4451 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 4501 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 4551 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATTTCAAGAA GATCCTTTGA 4601 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 4651 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTATA 4701 GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4751 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT 4801 GTACCCTCTG GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG 4851 ATTGCGGCAG TTTTTCGGGT GGTTTGTTGC CATTTTTACC TGTCTGCTGC 4901 CGTGATCGCG CTGAACGCGT TTTAGCGGTG CGTACAATTA AGGGATTATG 4951 GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA AACCGCAGTA 5001 CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCT Vector sequence updated 10-May-06.