pET-9a Vector Sequence This vector is no longer available from Promega Corporation. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pET-9a Vector Sequence Reference Points: Base Pairs 4338 T7 RNA polymerase terminator 404-446 BamHI cloning site 510 Nde I cloning site 550 Ribosome binding site (RBS) 560-565 T7 RNA polymerase promoter (-17 to +3) 612-631 T7 RNA polymerase transcription initiation site 615 pBR322 plasmid replication origin 2789 Kanamycin resistance gene (KanR) 3470-4335 .. 1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT 51 CACAGTTAAA TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT 101 GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG 151 CTTGGTTATG CCGGTACTGC CGGGCCTCTT GCGGGATATC GTCCATTCCG 201 ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA TGCGTTGATG 251 CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG 301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA 351 TCATGGCGAC CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT 401 CAGCAAAAAA CCCCTCAAGA CCCGTTTAGA GGCCCCAAGG GGTTATGCTA 451 GTTATTGCTC AGCGGTGGCA GCAGCCAACT CAGCTTCCTT TCGGGCTTTG 501 TTAGCAGCCG GATCCGCGAC CCATTTGCTG TCCACCAGTC ATGCTAGCCA 551 TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGAAAC 601 CGTTGTGGTC TCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC 651 TCGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG 701 TGCGGTTGCT GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG 751 CTCGCCACTT CGGGCTCATG AGCGCTTGTT TCGGCGTGGG TATGGTGGCA 801 GGCCCCGTGG CCGGGGGACT GTTGGGCGCC ATCTCCTTGC ATGCACCATT 851 CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC 901 TAATGCAGGA GTCGCATAAG GGAGAGCGTC GACCGATGCC CTTGAGAGCC 951 TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC 1001 CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG 1051 CAGCGCTCTG GGTCATTTTC GGCGAGGACC GCTTTCGCTG GAGCGCGACG 1101 ATGATCGGCC TGTCGCTTGC GGTATTCGGA ATCTTGCACG CCCTCGCTCA 1151 AGCCTTCGTC ACTGGTCCCG CCACCAAACG TTTCGGCGAG AAGCAGGCCA 1201 TTATCGCCGG CATGGCGGCC GACGCGCTGG GCTACGTCTT GCTGGCGTTC 1251 GCGACGCGAG GCTGGATGGC CTTCCCCATT ATGATTCTTC TCGCTTCCGG 1301 CGGCATCGGG ATGCCCGCGT TGCAGGCCAT GCTGTCCAGG CAGGTAGATG 1351 ACGACCATCA GGGACAGCTT CAAGGATCGC TCGCGGCTCT TACCAGCCTA 1401 ACTTCGATCA TTGGACCGCT GATCGTCACG GCGATTTATG CCGCCTCGGC 1451 GAGCACATGG AACGGGTTGG CATGGATTGT AGGCGCCGCC CTATACCTTG 1501 TCTGCCTCCC CGCGTTGCGT CGCGGTGCAT GGAGCCGGGC CACCTCGACC 1551 TGAATGGAAG CCGGCGGCAC CTCGCTAACG GATTCACCAC TCCAAGAATT 1601 GGAGCCAATC AATTCTTGCG GAGAACTGTG AATGCGCAAA CCAACCCTTG 1651 GCAGAACATA TCCATCGCGT CCGCCATCTC CAGCAGCCGC ACGCGGCGCA 1701 TCTCGGGCAG CGTTGGGTCC TGGCCACGGG TGCGCATGAT CGTGCTCCTG 1751 TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT TGCCTTACTG GTTAGCAGAA 1801 TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC TGCAAAACGT 1851 CTGCGACCTG AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA 1901 AGTCTGGAAA CGCGGAAGTC AGCGCCCTGC ACCATTATGT TCCGGATCTG 1951 CATCGCAGGA TGCTGCTGGC TACCCTGTGG AACACCTACA TCTGTATTAA 2001 CGAAGCGCTG GCATTGACCC TGAGTGATTT TTCTCTGGTC CCGCCGCATC 2051 CATACCGCCA GTTGTTTACC CTCACAACGT TCCAGTAACC GGGCATGTTC 2101 ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC ATCGGTATCA 2151 TTACCCCCAT GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA 2201 CAGGAAAAAA CCGCCCTTAA CATGGCCCGC TTTATCAGAA GCCAGACATT 2251 AACGCTTCTG GAGAAACTCA ACGAGCTGGA CGCGGATGAA CAGGCAGACA 2301 TCTGTGAATC GCTTCACGAC CACGCTGATG AGCTTTACCG CAGCTGCCTC 2351 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 2401 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 2451 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG 2501 TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG 2551 CAGATTGTAC TGAGAGTGCA CCATTGCGGT GTGAAATACC GCACAGATGC 2601 GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA 2651 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 2701 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 2751 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 2801 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 2851 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 2901 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 2951 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 3001 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 3051 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 3101 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 3151 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 3201 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 3251 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 3301 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 3351 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 3401 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 3451 CACGTTAAGG GATTTTGGTC ATGAACAATA AAACTGTCTG CTTACATAAA 3501 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCTTGCT 3551 CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA 3601 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGATTGTA 3651 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG 3701 TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA 3751 TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 3801 ATGGTTACTC ACCACTGCGA TCCCCGGGAA AACAGCATTC CAGGTATTAG 3851 AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC 3901 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 3951 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG 4001 TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA 4051 GTCTGGAAAG AAATGCATAA GCTTTTGCCA TTCTCACCGG ATTCAGTCGT 4101 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT 4151 TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG 4201 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 4251 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT 4301 TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAAGAA Vector sequence updated 23-Oct-02.