pALTERŪ Control vector sequence Note: Genetic elements of Promega's plasmid vectors are quality tested for their functionality. The sequence of the multiple cloning site is confirmed using restriction enzyme digestion, but the identity of every base pair of the plasmid has not been established by DNA sequencing. As a result, minor errors may exist in the sequence information provided. This vector can be obtained from Promega Corporation, Madison WI. Call one of the following numbers for order or technical information. Order or Technical 800-356-9526 Outside U.S. 608-274-4330 pALTER Control vector sequence reference points: 1. 5676 bases 2. T7 RNA polymerase transcription initiation site 1 3. SP6 RNA polymerase transcription initiation site 65 4. tac transcription initiation site N/A 5. T7 RNA polymerase promoter 5649-6 6. SP6 RNA polymerase promoter 60-82 7. tac promoter N/A 8. T7 translation start codon N/A 9. tac and SP6 translation start codon N/A 10. Multiple cloning sites 5-57 11. lacZ start codon 104 12. lac operon sequences 5497-5657, 90-319 13. lac operator 124-140 14. Beta-Lactamase (Ampr) coding region 3746-4602 15. Tetr gene coding region 537-1724 16. chloramphenicol acetyltransferase (Cmr) coding region N/A 17. phage f1 region 5041-5496 18. Binding site of pUC\M13 Forward sequencing primer 5617-5633 19. Binding site of pUC\M13 Reverse sequencing primer 100-116 20. Binding site of Ampicillin Repair Oligonucleotide 4050-4069 21. Binding site of Tetracycline Repair Oligonucleotide 620-647 22. Binding site of Chloramphenicol Repair Oligonucleotide N/A Use the T7 or pUC/M13 forward primers to sequence ssDNA produced by the pALTER_-1 vector pALTER is a registered trademark of Promega Corporation, Madison, WI. .. 1 GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCGGCATG 51 CAAGCTTGAG TATTCTATAG TGTCACCTAA ATAGCTTGGC GTAATCATGG 101 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 151 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 201 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA 251 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 301 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 351 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGAATTA 451 ATTCTCATGT TTGACAGCTT ATCATCGATT AGCTTTAATG CGGTAGTTTA 501 TCACAGTTAA ATTGCTAACG CAGTCAGGCA CCGTGTATGA AATCTAACAA 551 TGCGCTCATC GTCATCCTCG GCACCGTCAC CCTGGATGCT GTAGGCATAG 601 GCTTGGTTAT GCCGGTACTG CCGGGCCTCT TGCGGGATAT CGTCCATTCC 651 GACAGCATCG CCAGTCACTA TGGCGTGCTG CTAGCGCTAT ATGCGTTGAT 701 GCAATTTCTA TGCGCACCCG TTCTCGGAGC ACTGTCCGAC CGCTTTGGCC 751 GCCGCCCAGT CCTGCTCGCT TCGCTACTTG GAGCCACTAT CGACTACGCG 801 ATCATGGCGA CCACACCCGT CCTGTGGATT CTCTACGCCG GACGCATCGT 851 GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG 901 ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT 951 TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG 1001 CGCCATCTCC TTACATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC 1051 TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG 1101 CGCCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG 1151 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA 1201 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG 1251 GACCGCTTTC GCTGGAGCGC GACGATGATC GGCCTGTCGC TTGCGGTATT 1301 CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT CCCGCCACCA 1351 AACGTTTCGG CGAGAAGCAG GCCATTATCG CCGGCATGGC GGCCGACGCG 1401 CTGGGCTACG TCTTGCTGGC GTTCGCGACG CGAGGCTGGA TGGCCTTCCC 1451 CATTATGATT CTTCTCGCTT CCGGCGGCAT CGGGATGCCC GCGTTGCAGG 1501 CCATGCTGTC CAGGCAGGTA GATGACGACC ATCAGGGACA GCTTCAAGGA 1551 TCGCTCGCGG CTCTTACCAG CCTAACTTCG ATCACTGGAC CGCTGATCGT 1601 CACGGCGATT TATGCCGCCT CGGCGAGCAC ATGGAACGGG TTGGCATGGA 1651 TTGTAGGCGC CGCCCTATAC CTTGTCTGCC TCCCCGCGTT GCGTCGCGGT 1701 GCATGGAGCC GGGCCACCTC GACCTGAATG GAAGCCGGCG GCACCTCGCT 1751 AACGGATTCA CCACTCCAAG AATTGGAGCC AATCAATTCT TGCGGAGAAC 1801 TGTGAATGCG CAAACCAACC CTTGGCAGAA CATATCCATC GCGTCCGCCA 1851 TCTCCAGCAG CCGCACGCGG CGCATCTCGG GCAGCGTTGG GTCCTGGCCA 1901 CGGGTGCGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG 1951 GGGTTGCCTT ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT 2001 GAAGCGACTG CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC AACATGAATG 2051 GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG GAAACGCGGA AGTCAGCGCC 2101 CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC TGGCTACCCT 2151 GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG 2201 ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA 2251 ACGTTCCAGT AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA 2301 TCCTCTCTCG TTTCATCGGT ATCATTACCC CCATGAACAG AAATTCCCCC 2351 TTACACGGAG GCATCAAGTG ACCAAACAGG AAAAAACCGC CCTTAACATG 2401 GCCCGCTTTA TCAGAAGCCA GACATTAACG CTTCTGGAGA AACTCAACGA 2451 GCTGGACGCG GATGAACAGG CAGACATCTG TGAATCGCTT CACGACCACG 2501 CTGATGAGCT TTACCGCAGC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA 2551 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 2601 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 2651 GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT 2701 ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 2751 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 2801 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 2851 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 2901 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 2951 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 3001 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 3051 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 3101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 3151 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 3201 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 3251 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 3301 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 3351 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 3401 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 3451 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 3501 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 3551 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 3601 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 3651 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 3701 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTTGGCT GACAGTTACC 3751 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 3801 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 3851 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 3901 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 3951 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 4001 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 4051 TTGCCATTGC GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 4101 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 4151 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 4201 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 4251 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 4301 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 4351 GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 4401 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 4451 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 4501 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 4551 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 4601 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 4651 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 4701 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 4751 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG 4801 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 4851 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 4901 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 4951 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA 5001 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG AAATTGTAAA 5051 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TATTTGTTAA ATCAGCTCAT 5101 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 5151 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT 5201 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG 5251 GCGATGGCCC ACTACGTGAA CCATCACCCA AATCAAGTTT TTTGCGGTCG 5301 AGGTGCCGTA AAGCTCTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG 5351 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 5401 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 5451 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCAT 5501 TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 5551 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA 5601 GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC 5651 AGTGAATTGT AATACGACTC ACTATA