Promega discontinued vectors

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Bacterial Expression

Cloning

Discontinued

Mutagenesis

Mammalian Expression

pGEM^®-luc,
ΦX174 & pSP

Reporter

RNA Interference

T Vectors

Complete Vector List

Technical Resources

All Discontinued Vectors

EMBL Lambda pALTER pCAT pET pGEM		pGEMEX phRG phRL PinPoint psiLentGene pSP psiSTRIKE


EMBL
Enlarge map		EMBL3 Genomic Cloning Lambda replacement vector derived from lambda 1059. For sequence see GenBank^® Acc.#: U02453 (right arm) and U02425 (left arm) MCR Restriction Enzymes: SalI, BamHI, EcoRI

Enlarge map		EMBL4 Genomic Cloning Lambda replacement vector derived from lambda 1059. Identical to EMBL3 except that multiple cloning region is in the reverse orientation. EMBL4 has same stuffer fragment as EMBL3. GenBank^® Acc.#: None MCR Restriction Enzymes: SalI, BamHI, EcoRI

Lambda
Enlarge map		LambdaGem-11 Genomic Cloning Multifunctional replacement type genomic cloning vector that accepts DNA fragments of 9-23kb. For sequence see GenBank^® Acc.#: U02427 (right arm) and U02426 (left arm) MCR restriction enzymes: SfiI, SacI, XhoI, BamHI, AvrII, EcoRI, XbaI

Enlarge map		LambdaGem-12 Genomic Cloning Lambda replacement vector derived from lambda 1059. LambdaGem-12 arms are identical to EMBL3 arms (above) except that they have different multiple cloning regions containing T7 and SP6 RNA polymerase promoters and recognition sites listed below. LambdaGem-12 has a different stuffer fragment than EMBL3. MCR Restriction Enzymes: SfiI, SacI, NotI, BamHI, EcoRI, XhoI

Enlarge map		Lambda gt10 cDNA cloning vector. The polylinker region is GenBank^® Acc.#: U02447 MCR Restriction Enzymes: None

Enlarge map		Lambda gt11 cDNA cloning vector. Protocol: None The polylinker region is X16973 The lac region is X04125 MCR Restriction Enzymes: None

Enlarge map		Lambda gt11 Sfi-Not cDNA cloning vector. Protocol: None GenBank^® Acc.#: None MCR Restriction Enzymes: None

pALTER^® Vectors
(map not available)		pALTER^®-Control Mutagenesis vector. Sequence and reference points Protocol: Altered Sites^® II in vitro Mutagenesis Systems, TM001 GenBank^® Acc.#: None MCR Restriction Enzymes: None

Enlarge map		pALTER^®-1 Contains an multiple cloning site flanked by opposing SP6 and T7 RNA polymerase promoters, and inserted into the lacZ α-peptide-encoding DNA. Sequence and reference points MCR sequence Protocol: Altered Sites^® II in vitro Mutagenesis Systems, TM001 GenBank^® Acc.#: X65334 MCR Restriction Enzymes: EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI, HindIII

Enlarge map		pALTER^®-EX1 Contains a novel polylinker that allows protein expression in vivo and in vitro. Sequence and reference points MCR sequence Protocol: Altered Sites^® II in vitro Mutagenesis Systems, TM001 GenBank^® Acc.#: U47102 MCR restriction enzymes: EcoRI, HpaI, NdeI, Nco I, StuI, XbaI, PstI, NotI, SphI, NsiI, BamHI, HindIII

Enlarge map		pALTER^®-EX2 Contains the same cloning sites and transcription/translation signals as pALTER^®-EX1, but used the p15a origin or replication. Sequence and reference points MCR sequence Protocol: Altered Sites^® II in vitro Mutagenesis Systems, TM001 GenBank^® Acc.#: U47103 MCR restriction enzymes: EcoRI, HpaI, NcoI, StuI, PstI, NotI, SphI, NsiI, NdeI, BamHI, HindIII

pCAT^® Vectors
Enlarge map		pCAT^®-Basic GenBank^® Acc.#: X65322

Enlarge map		pCAT^®-Control GenBank^® Acc.#: X65321

Enlarge map		pCAT^®-Enhancer GenBank^® Acc.#: X65319

Enlarge map		pCAT^®-Promoter GenBank^® Acc.#: X65320

pET Vectors
Enlarge map		pET-5a Bacterial expression. Sequence and reference points GenBank^® Acc.#: None MCR restriction enzymes: None

Enlarge map		pET-5b Bacterial expression. Sequence and reference points GenBank^® Acc.#: None MCR restriction enzymes: None

Enlarge map		pET-5c Bacterial expression. Sequence and reference points GenBank^® Acc.#: None MCR restriction enzymes: None

Enlarge map		pET-9a Bacterial expression. Sequence and reference points GenBank^® Acc.#: None MCR restriction enzymes: None

Enlarge map		pET-9b Bacterial expression. Sequence and reference points GenBank^® Acc.#: None MCR restriction enzymes: None

Enlarge map		pET-9c Bacterial expression. Sequence and reference points GenBank^® Acc.#: None MCR restriction enzymes: None

pGEM^® Vectors
Enlarge map		pGEM^®-1 Sequence and reference points GenBank^® Acc.#: X65300 MCR restriction enzymes: HindIII, PstI, SalI, AccI, SmaI, HincII, XbaI, BamHI, AvaI, SacI, EcoRI

Enlarge map		pGEM^®-2 Sequence and reference points GenBank^® Acc.#: X65301 MCR restriction enzymes: EcoRI, SacI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, HindIII

Enlarge map		pGEM^®-3 Sequence and reference points GenBank^® Acc.#: X65302 MCR restriction enzymes: HindIII, SphI, PstI, SalI, AccI, HincII, XbaI, AvaI, BamHI, SmaI, KpnI, SacI, EcoRI

Enlarge map		pGEM^®-4 Vector Sequence and reference points Protocol: None GenBank^® Acc.#: X6530 3 MCR restriction enzymes: EcoRI, SacI, KpnI, AvaI, SmaI, XbaI, BamHI, SalI, AccI, HincII, PstI, SphI, HindIII

map not available		pGEM^®-15Zf(–) Cloning. Sequence and reference points MCR restriction enzymes: None

pGEMEX^® Vectors
Enlarge map		pGEMEX^®-1 Designed for cloning, as templates for in vitro transcription and for production of ssDNA. Sequence and reference points MCR sequence GenBank^® Acc.#: X65317 MCR restriction enzymes: HindIII, NsiI, SphI, NotI, ApaI, XhoI, BamHI, SalI, EcoRI, SacI, SfiI

Enlarge map		pGEMEX^®-2 Designed for cloning, as templates for in vitro transcription and for production of ssDNA. Sequence and reference points MCR sequence GenBank^® Acc.#: X65318 MCR restriction enzymes: HindIII, NsiI, SphI, NotI, ApaI, XhoI, BamHI, SalI, EcoRI, SacI, SfiI

phRG Vectors
Enlarge map		phRG-B (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points MCR sequence GenBank^® Acc.#: AF362550 MCR restriction enzymes: KpnI, SacI, MluI, NheI,Sma I, XhoI, BglII, HindIII

Enlarge map		phRG-TK (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points GenBank^® Acc.#: AF362551

phRL Vectors
Enlarge map		phRL-CMV (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points GenBank^® Acc.#: AF362549

Enlarge map		phRL-null (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points MCR sequence GenBank^® Acc.#: AF362546 MCR restriction enzymes: Bgl II, XhoI, SacI, HindIII, EcoICRI, NdeI, SphI, SpeI, NarI, SalI, MluI, EcoRI, SmaI, PstI

Enlarge map		phRL-SV40 (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points Protocol: Synthetic Renilla Luciferase Reporter Vectors, TM237 GenBank^® Acc.#: AF362548

Enlarge map		phRL-TK (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points GenBank^® Acc.#: AF362545 MCR restriction enzymes: None

Enlarge map		phRL-TK(Int–) (discontinued, 1/08) Mammalian expression and reporter vector. Sequence and reference points GenBank^® Acc.#: AF362547 MCR restriction enzymes: None

PinPoint™ Vectors
Enlarge map		PinPoint™ Control Bacterial expression. Sequence and reference points Protocol: E. coli T7 S30 Extract System for Circular DNA, TB219 GenBank^® Acc.#: None MCR restriction enzymes: NruI, PvuI, HindIII, NotI

Enlarge map		PinPoint™ Xa Control Bacterial expression. Sequence and reference points MCR sequence: None Protocol: PinPoint™ Xa Protein Purification System, TM028 GenBank^® Acc.#: None

Vector map and sequence are nearly identical to PinPoint™ Xa-1 (above), but contain one additional adenosine at position 394.		PinPoint™ Xa-2 Bacterial expression. MCR sequence Protocol: PinPoint™ Xa Protein Purification System, TM028 GenBank^® Acc.#: U47627 MCR restriction enzymes: NruI, PvuI, HindIII, BamHI, Acc65I, KpnI, BglII, EcoRV, SmaI, NotI

Vector map and sequence are nearly identical to PinPoint™ Xa-1 (above), but contain two additional adenosines at position 394.		PinPoint™ Xa-3 Bacterial expression. MCR sequence Protocol: PinPoint™ Xa Protein Purification System, TM028 GenBank^® Acc.#: U47628 MCR restriction enzymes: NruI, PvuI, HindIII, BamHI, Acc65I, KpnI, BglII, EcoRV, SmaI, NotI

Enlarge map		PinPoint™ Xa-1 T-Vector This vector is designed for cloning PCR products. Sequence and reference points MCR sequence GenBank^® Acc.#: None MCR restriction enzymes: NruI, PvuI, HindIII, BamHI, Acc65I, KpnI, BglII, SmaI, NotI

psiLentGene™ Vectors
Enlarge map		psiLentGene™ Basic Sequence and reference points GenBank^® Acc.#: AY508731

Enlarge map		psiLentGene™ Hygromycin Sequence and reference points GenBank^® Acc.#: AY508734

Enlarge map		psiLentGene™ Neomycin Sequence and reference points GenBank^® Acc.#: AY508733

Enlarge map		psiLentGene™ Puromycin Sequence and reference points GenBank^® Acc.#: AY508732

pSP Vectors
Enlarge map		pSP64 Cloning, in vitro transcription. Sequence and reference points GenBank^® Acc.#: X65327 MCR restriction enzymes: HindIII, PstI, SalI, AccI, HincII,XbaI, AvaI, BamHI, SmaI, SacI, EcoRI

Enlarge map		pSP65 Cloning, in vitro transcription. Sequence and reference points GenBank^® Acc.#: X65329 MCR restriction enzymes: EcoRI, SacI, AvaI, SmaI, XbaI, BamHI, SalI, AccI, HincII, PstI, HindIII

Enlarge map		pSP70 Cloning, in vitro transcription. Sequence and reference points GenBank^® Acc.#: X65330 MCR restriction enzymes: XhoI, PvuI, HindIII, EcoR I, ClaI, EcoRV, BglII

Enlarge map		*pSP-luc+* Luciferase cassette vector. Sequence and reference points MCR sequence Protocol: None GenBank^® Acc.#: U47122 MCR restriction enzymes: KpnI, NheI, BglII, AvrII, HindIII, NcoI, XbaI, EcoRI, ClaI, EcoRV, XhoI

psiSTRIKE™
Enlarge map		psiSTRIKE™ Basic Designed for in vivo expression of siRNAs. Sequence and reference points GenBank^® Acc.#: AY497505

Enlarge map		psiSTRIKE™ hMGFP Designed for in vivo expression of short hairpin RNAs. Sequence and reference points GenBank^® Acc.#: AY585853

Enlarge map		psiSTRIKE™ Hygromycin Designed for in vivo expression of siRNAs. Sequence and reference points GenBank^® Acc.#: AY497506

Enlarge map		psiSTRIKE™ Neomycin Designed for in vivo expression of siRNAs. Sequence and reference points GenBank^® Acc.#: AY497508

Enlarge map		psiSTRIKE™ Puromycin Designed for in vivo expression of siRNAs. Sequence and reference points GenBank^® Acc.#: AY497507

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