Fast and Easy Luminescent Assay to Measuring Monoamine Oxidase

The Mao-Glo™ Assay is a homogenous, luminescent assay that measures MAO A and MAO B activity from native and recombinant sources.

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About Monoamine Oxidase Assay
Assay Sensitivity and Linearity
Conversion of the MAO substrate
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About the Monoamine Oxidase Assay
Monoamine oxidase catalyze the oxidative deamination of biogenic and xenobiotic amines. The ability to measure MAO activity and the effects of test compounds on that activity is critical for selecting MAO inhibitors, discovering potential drug-drug interactions and assessing MAO-catalyzed detoxification and biotransformation. Current methods for measuring MAO activity are typically time-consuming and expensive. The MAO-Glo™ Assay is a simple, homogeneous and robust two-step bioluminescent-coupled assay.

Assay Sensitivity and Linearity Light intensity of the MAO-Glo™ Assay is proportional to enzyme activity over a >10,000-fold range. the Signal:noise ratio was determinded by dividing  the net luminescent signal by the standard deviation of the background. the limit of detection of is the concentration of enzyme requuired to generate a signal:noise ratio of 3 (green line). The MAO-Glo™ Assay is up to one hundred times more sensitive than competing fluorescent assays. View larger image

Conversion of the MAO substrate Monoamine oxidase acts on the luminogenic MAO Substrate to produce methyl ester Luciferin, which is then converted into light by the esterase and luciferase in the reconstituted Luciferin Detection reagent. View larger image

Article: High-Throughput Compound Profiling Using Tecan Instrumentation
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More information on Cytochrome P450s
More information on P-glycoprotein (Pgp)

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