Speed up your cloning reactions!

TSAP Thermosensitive Alkaline Phosphatase New!

Dephosphorylate and inactivate in 30 minutes

Description: TSAP - Thermosensitive Alkaline Phosphatase catalyzes the removal of 5´ phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3´ overhangs, 5´ overhangs, and blunt ends. It is also useful for preparing DNA for 5' end-labeling by removing existing phosphate groups from the 5’ end.

• Inactivated by heating at 74°C for 15 minutes
• DNA clean up is not required before proceeding to ligation
• Fully active in all Promega restriction enzyme reaction buffers
• Restriction digestion, dephosphorylation and ligation reactions can be performed without the need for DNA clean up or buffer swaps.

 
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