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Protease-Glo™ Oligonucleotide Designer is a web application that will assist in converting a desired amino acid sequence, representing a protease recognition sequence of interest, into two complementary DNA oligonucleotide sequences. Simply input the single amino acid code of the recognition sequence for your protease of interest, and the tool will design the oligonucleotide sequences. We strongly recommend that you use the Protease-Glo™ Oligonucleotide Designer tool. In addition the Designer Tool will append the required ‘sticky ends’ to the two sequences improving proper ligation into the pGloSensor™-10F Linear Vector.
The Protease-Glo™ Assay is a novel method to detect and measure protease activities using a genetically engineered firefly (Photinus pyralis) luciferase and represents one example of the GloSensor™ platform technology. To learn more about the new Protease-Glo™ Assay, please see the
Protease-Glo™ Assay Technical Manual. To learn more about the GloSensor Technology, please go to the
GloSensor™ Technology Web page.
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